Human choriocarcinoma cell line JEG-3 produces and secretes active retinoids from retinol

Blanchon, Loı̈c; Sauvant, Patrick; Båvik, Claes; Gallot, Denis; Charbonné, F; Alexandre-Gouabau, Marie Cécile; Lémery, D.; Jacquetin, B.; Dastugue, B.; Ward, Simon; Sapin, Vincent

doi:10.1093/molehr/8.5.485

Cited by 13 publications

(6 citation statements)

References 61 publications

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“…Cells constitutively expressing HLA-G (choriocarcinoma JEG-3 and melanoma FON + cell lines) were grown in DMEM (Gibco, Carlsbad, CA) and RPMI-1640 (Sigma-Aldrich, Lyon, France) supplemented mediums, respectively 55 , 56 . One microgram of each construction was transfected into sub-confluent JEG-3 cells by Lipofectamine 2000 Transfection Reagent (Invitrogen) and FON + cells by Lipofectamine LTX Reagent with Plus Reagent (Invitrogen), according to manufacturer’s instructions.…”

Section: Methodsmentioning

confidence: 99%

The genetic diversity within the 1.4 kb HLA-G 5′ upstream regulatory region moderately impacts on cellular microenvironment responses

Dias

Bertol

Poras

et al. 2018

Sci Rep

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The HLA-G 5’URR extending 1.4 kb from the ATG presents a unique set of regulatory elements among HLA genes. Several variable sites have been described that coincide with or are close to these elements, thus HLA-G 5′URR polymorphism might influence the HLA-G expression level. We cloned the ten most frequent HLA-G 5′URR haplotypes to evaluate their activity on a luciferase reporter gene in HLA-G+ cell lines (JEG-3/choriocarcinoma and FON+/melanoma). We also investigated associations between the plasma HLA-G (sHLA-G) levels and the HLA-G 5′URR variability in 157 healthy individuals. Cell lines were transfected with pGL3-Basic vector constructions containing HLA-G 5′URR sequences. The G010101a (in JEG-3) and G010101b (in FON+) haplotypes exhibited higher promoter activity, whereas the G010101d (in JEG-3) and G010102a (in FON+) haplotypes exhibited lower promoter activity. In the presence of HLA-G inducers (interferon-β and progesterone) or repressors (cyclopamine) HLA-G promoter activity was modulated, but certain haplotypes exhibited differential responses. No strict association was observed between plasma sHLA-G levels and the 5′URR haplotypes or genotypes; however, the G010101b haplotype was underrepresented among HLA-G-negative plasmas. Therefore, the HLA-G 5′URR polymorphism may have an impact on the modulation of HLA-G gene expression, but alone provides a limited predictive value for sHLA-G levels in vivo.

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Section: Methodsmentioning

confidence: 99%

The genetic diversity within the 1.4 kb HLA-G 5′ upstream regulatory region moderately impacts on cellular microenvironment responses

Dias

Bertol

Poras

et al. 2018

Sci Rep

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“…Human trophoblast-derived choriocarcinoma BeWo cells and JEG3 cells were purchased from American Type Culture Collection and grown in FK12 and DMEM, respectively, as described previously [37,38]. The first trimester trophoblast-derived HTR-8/SVneo cell line, kindly provided by Dr Peeyush K. Lala (Western University, Canada), was grown in RPMI 1640, as described previously [39].…”

Section: Trophoblast Cell Lines and Primary Cell Isolation And Culturementioning

confidence: 99%

Human trophoblast-derived hydrogen sulfide stimulates placental artery endothelial cell angiogenesis†

Chen

Feng

Hodges

et al. 2017

Biology of Reproduction

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Endogenous hydrogen sulfide (H2S), mainly synthesized by cystathionine β-synthase (CBS) and cystathionine γ-lyase (CTH), has been implicated in regulating placental angiogenesis; however, the underlying mechanisms are unknown. This study was to test a hypothesis that trophoblasts synthesize H2S to promote placental angiogenesis. Human choriocarcinoma-derived BeWo cells expressed both CBS and CTH proteins, while the first trimester villous trophoblast-originated HTR-8/SVneo cells expressed CTH protein only. The H2S producing ability of BeWo cells was significantly inhibited by either inhibitors of CBS (carboxymethyl hydroxylamine hemihydrochloride, CHH) or CTH (β-cyano-L-alanine, BCA) and that in HTR-8/SVneo cells was inhibited by CHH only. H2S donors stimulated cell proliferation, migration, and tube formation in ovine placental artery endothelial cells (oFPAECs) as effectively as vascular endothelial growth factor. Co-culture with BeWo and HTR-8/SVneo cells stimulated oFPAEC migration, which was inhibited by CHH or BCA in BeWo but CHH only in HTR-8/SVneo cells. Primary human villous trophoblasts (HVT) were more potent than trophoblast cell lines in stimulating oFPAEC migration that was inhibited by CHH and CHH/BCA combination in accordance with its H2S synthesizing activity linked to CBS and CTH expression patterns. H2S donors activated endothelial nitric oxide synthase (NOS3), v-AKT murine thymoma viral oncogene homolog 1 (AKT1), and extracellular signal-activated kinase 1/2 (mitogen-activated protein kinase 3/1, MAPK3/1) in oFPAECs. H2S donor-induced NOS3 activation was blocked by AKT1 but not MAPK3/1 inhibition. In keeping with our previous studies showing a crucial role of AKT1, MAPK3/1, and NOS3/NO in placental angiogenesis, these data show that trophoblast-derived endogenous H2S stimulates placental angiogenesis, involving activation of AKT1, NOS3/NO, and MAPK3/1.

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“…[24][25][26] Expression of receptor to retinoic acid has been shown in clinical samples of CCA, and JEG-3, a human CCA cell line, also produces active retinoids. [27][28][29][30] In monolayer culture of a CCA cell line, with the use of a 3-(4.5-dimethylthiazol-2-yl)-2.5-diphenyltetrozoliumbromide-based cell proliferation assay, it was shown that ATRA could inhibit cell proliferation and enhance the effect of standard chemotherapeutic agents, such as methotrexate or actinomycin D. 27 Retinoids may thus be useful in the treatment for trophoblastic malignancy, including CCA. The mechanisms of the action of retinoic acid in CCA, however, remain unclear.…”

Section: Discussionmentioning

confidence: 99%

Effect of all-transretinoic acid on tissue dynamics of choriocarcinoma cell lines: an organotypic model

Chiu

Feng

Benbrook³

et al. 2006

J Clin Pathol

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Background: All-trans retinoic acid (ATRA) is a natural vitamin A derivative that has a profound effect on the regulation of cell growth, differentiation and death. Aim: To investigate the tissue dynamic and cellular invasion effects of ATRA in choriocarcinoma (CCA), an aggressive trophoblastic tumour, by using a three-dimensional organotypic culture model system and cell invasion assay, respectively. Methods: An organotypic culture model of two CCA cell lines, JAR and JEG, was established. The effects of 1 mM ATRA on proliferation, differentiation and apoptosis on this CCA model were assessed by morphological assessment of the mitotic and apoptotic figures as well as by Ki-67 and caspase-related M30 cytoDeath antibody immunohistochemistry and the terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labelling (TUNEL) assay. The effect of ATRA on p53 and its regulated protein product, WAF1/Cip1, was also evaluated with DO7 and p21 WAF1 antibodies, respectively. Moreover, the effect of ATRA on cellular (CCA) invasion was also investigated with Cell Invasion Kit on the JEG cell line. WAF1 (p,0.0001) immunoreactivity. 1.5 mM ATRA was found to effectively inhibit JEG cell invasion in the cell invasion assay. Conclusion: ATRA treatment was found to inhibit invasion and proliferation and enhance apoptosis, probably by the activation of caspases and induction of differentiation. ATRA and synthetic retinoids may be alternative agents for the treatment of CCA.

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Human choriocarcinoma cell line JEG-3 produces and secretes active retinoids from retinol

Cited by 13 publications

References 61 publications

The genetic diversity within the 1.4 kb HLA-G 5′ upstream regulatory region moderately impacts on cellular microenvironment responses

The genetic diversity within the 1.4 kb HLA-G 5′ upstream regulatory region moderately impacts on cellular microenvironment responses

Human trophoblast-derived hydrogen sulfide stimulates placental artery endothelial cell angiogenesis†

Effect of all-transretinoic acid on tissue dynamics of choriocarcinoma cell lines: an organotypic model

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