Human Cancer Cell Membrane-Cloaked Fe₃O₄ Nanocubes for Homologous Targeting Improvement

Abstract

Surface modification of nanoparticles with cellular protein components is a new biomimetic modification strategy, which utilizes the inherent affinity between homologous cells to introduce the same surface molecules into nanoparticles to improve the targeting performance. In this study, oleic acid (OA)-coated Fe 3 O 4 nanocubes were prepared by a high-temperature thermal decomposition method and modified by 3, 4dihydroxyphenylpropionic acid (DHCA); then, HeLa cell membranes were introduced onto the surface of the nanocubes through mixed coextrusion to try to endow them with the targeting function of natural cells. The results show that the prepared Fe 3 O 4 nanocubes have high monodispersity, excellent water solubility, and biocompatibility. Moreover, the Fe 3 O 4 nanocubes encapsulated by cellular protein show an obvious core−shell structure and the specific targeting property to HeLa cells is improved significantly, which is expected to be used in clinical targeted diagnosis and treatment of cancer.

Inhibiting mTTR Aggregation/Fibrillation by a Chaperone-like Hydrophobic Amino Acid-Conjugated SPION

“…Among diverse applications of NPs in the biomedicine arena, they are now accepted as artificial chaperones that could halt protein aggregation efficiently. Nevertheless, most of these studies have focused on polymeric NPs. , …”

“…Nevertheless, most of these studies have focused on polymeric NPs. 8,44 The successful synthesis of amino acid-conjugated SPIONs was confirmed by a series of physical and chemical analyses as follows: monodisperse SPIONs with a narrow size distribution are detectable in TEM images (Figure 1a−d). The majority of the particles occurred in spherical morphologies with a 9.2 nm diameter on average.…”

“…Thus, enormous demand has emerged for taking the benefit of NPs in the last decades. For instance, in the field of biomedicine, a promising number of NPs have been introduced as diagnostic and therapeutic agents lately. − Especially, superparamagnetic iron oxide NPs (SPIONs) have garnered much interest due to their remarkable biocompatibility and superparamagnetic properties for bioseparation, therapy, and diagnosis. , Biodegradability is another privilege of the SPIONs. Since the removal of the NPs from the body remains a big challenge, SPIONs can be metabolized in the liver and excreted by the kidneys …”

Recent progress in cancer cell membrane-based nanoparticles for biomedical applications

“…However, magnetic NPs are prone to aggregation and oxidation, which leads to a decrease in heating efficiency [ 100 ]. In addition, magnetic NPs are usually recognized and cleared from the body, which prevents them from reaching their target [ 101 ]. Biomimetic cancer cell membrane technology can enhance the performance of magnetic iron oxide NPs (e.g., prevent oxidation, improve biocompatibility, enhance colloidal stability, and enhance targeting), enabling the ablation of tumor tissues by thermal energy [ 79 ].…”

Nanomaterials: small particles show huge possibilities for cancer immunotherapy

“…On the one hand, the modification of nanomaterials using tumor cell membranes well ensures the diversity of tumor antigens. On the other hand, the homing ability mediated by surface membrane proteins enhances the targeting of nanomaterials [ 43 , 44 ]. Moreover, the preparation of the relevant nanomaterials is significantly reduced due to the rapid proliferation of tumor cells and the simple extraction of their cell membranes.…”