Surface modification of nanoparticles with cellular protein components is a new biomimetic modification strategy, which utilizes the inherent affinity between homologous cells to introduce the same surface molecules into nanoparticles to improve the targeting performance. In this study, oleic acid (OA)-coated Fe 3 O 4 nanocubes were prepared by a high-temperature thermal decomposition method and modified by 3, 4dihydroxyphenylpropionic acid (DHCA); then, HeLa cell membranes were introduced onto the surface of the nanocubes through mixed coextrusion to try to endow them with the targeting function of natural cells. The results show that the prepared Fe 3 O 4 nanocubes have high monodispersity, excellent water solubility, and biocompatibility. Moreover, the Fe 3 O 4 nanocubes encapsulated by cellular protein show an obvious core−shell structure and the specific targeting property to HeLa cells is improved significantly, which is expected to be used in clinical targeted diagnosis and treatment of cancer.
Herein, we proposed
a drug-free strategy named cell surface shellization
to inhibit the motility of SKOV-3 and HeLa cells. We alternately deposited
two- or three-layer cationic polyelectrolyte (PE) and anionic PE films
on the surface of SKOV-3 and HeLa cells. Then, a mineral shell (calcium
carbonate, CaCO
3
) was formed on the surface of polymer
shells via electrostatic force and biomineralization. The CCK-8 assay
results and live/dead staining showed that the surface shells strongly
aggravated the cytotoxicity. The monolayer scratch wound migration
assay results and immunofluorescence staining results showed that
the shells, especially the mineral shells, could efficiently inhibit
the migration of SKOV-3 and HeLa cells without any anticancer drugs.
The immunofluorescence results of the three small G proteins of the
cells showed that the immunofluorescence intensity in SKOV-3 did not
change. Preliminary results from our laboratory showed an increase
in MMP-9 secreted by cancer cells after coating with films or mineral
shells. It suggests that mechanisms that inhibit cell migration are
related to the MMP signaling pathway. All the results indicated that
shellization (films or nanomineral shells) but not limited to calcification
can be used as one of the tools to change the function of cells.
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