Human Bocavirus: Prevalence and Clinical Spectrum at a Children’s Hospital

Arnold, John; Singh, Kumud K.; Spector, Stephen A.; Sawyer, Mark H.

doi:10.1086/505399

Cited by 206 publications

(283 citation statements)

References 14 publications

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“…Although IgG antibody present in a single serum sample does not reflect an acute infection, a high rate of IgG positive sera from healthy children does suggest that HBoV infection is more common in children than genomic detection has shown previously (Allander et al, 2005;Arnold et al, 2006;Bastien et al, 2006;Choi et al, 2006;Foulongne et al, 2006;Lin et al, 2007;Ma et al, 2006;Qu et al, 2007;Sloots et al, 2006;Weissbrich et al, 2006). Infection with B19 occurs in children in a high incidence asymptomatically.…”

Section: Discussionmentioning

confidence: 92%

ELISAs using human bocavirus VP2 virus-like particles for detection of antibodies against HBoV

Lin

Guan

Cheng

et al. 2008

Journal of Virological Methods

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Human bocavirus (HBoV) has been identified worldwide in children with lower respiratory tract infections with an incidence of approximately 2% −11%. The role of HBoV in pathogenesis, however, is largely unknown, and little is known about the epidemiology of the virus. To study the seroepidemiology of HBoV infection, the capsid protein was expressed in insect cells. Expression of the putative major capsid protein VP2 in insect cells led to the formation of virus-like particles exhibiting the typical icosahedral appearance of parvoviruses with a diameter of approximately 20 nm. The expressed particles were used to establish an ELISA method, and serum samples from groups of children of various ages in China were tested for IgG antibodies against HBoV. HBoV antibodies were detected in as high as 36% of healthy children under 9 years. Of children hospitalized with lower respiratory tract infections, 31% were seropositive, and all age groups of these children showed a significantly higher level of HBoV IgG antibody than their healthy counterparts. When divided into age cohorts, results showed that more than 48% of healthy children had seroconverted by age of 4. Thus, HBoV appears to be a common infection in children. The potential pathogenesis of this virus, especially its role in lower respiratory tract infections in children warrants further investigation.

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Section: Discussionmentioning

confidence: 92%

ELISAs using human bocavirus VP2 virus-like particles for detection of antibodies against HBoV

Lin

Guan

Cheng

et al. 2008

Journal of Virological Methods

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“…Control group participants were children <18 years of age (median Clinical data were obtained by using standardized forms to extract data from electronic charts. Underlying illnesses were defi ned as chronic pulmonary disease, congenital heart disease, immunodefi ciency, malignancy, neurologic disease, or gastrointestinal disease (8). To assess the severity of illness, we used the lowest ratio during the fi rst 24 hours of the partial pressure of oxygen in arterial blood (PaO 2) to the inspired oxygen fraction (FiO 2 ).…”

Section: The Studymentioning

confidence: 99%

Human Bocavirus and KI/WU Polyomaviruses in Pediatric Intensive Care Patients

Pol¹,

Wolfs²,

Jansen³

et al. 2009

Emerg. Infect. Dis.

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“…Most prevalence studies were done on samples from children with respiratory tract diseases, with rates observed between 1.5-19% (1,(4)(5)(6)(7)(8)(9)(10)(11)(12). Studies made on fecal specimens indicate that HBoV is also prevalent in children with acute gastroenteritis, with 0.8-16% of samples being HBoV + (4,(13)(14)(15). In addition, HBoV infection with complex clinical conditions in immunosuppressed patients has been suggested (16).…”

mentioning

confidence: 99%

Human Bocavirus NP1 Inhibits IFN-β Production by Blocking Association of IFN Regulatory Factor 3 with IFNB Promoter

Zhang

Zheng

Luo

et al. 2012

The Journal of Immunology

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Human bocavirus (HBoV) mainly infects young children. Although many infected children suffer from respiratory or gastroenteric tract diseases, an association between HBoV and these diseases is not definite. Because modulation of type I IFN is crucial for viruses to establish efficient replication, in this study, we tested whether HBoV modulates type I IFN production. We observed that a nearly full-length HBoV clone significantly reduced both Sendai virus (SeV)- and poly(deoxyadenylic-thymidylic) acid-induced IFN-β production. Further study showed that NP1 blocked IFN-β activation in response to SeV, poly(deoxyadenylic-thymidylic) acid, and IFN-β pathway inducers, including retinoic acid-inducible protein I, mitochondrial antiviral signaling protein, inhibitor of κB kinase ε, and TANK-binding kinase 1. In addition, NP1 interfered with IRF-3–responsive PRD(III-I) promoter activated by SeV and a constitutively active mutant of IRF-3 (IRF-3/5D). Although NP1 suppressed the IRF-3 pathway, it did not affect IRF-3 activation processes, including phosphorylation, dimerization, and nuclear translocation. Coimmunoprecipitation assays confirmed the interaction between NP1 and IRF-3. Additional deletion mutagenesis and coimmunoprecipitation assays revealed that NP1 bound to the DNA-binding domain of IRF-3, resulting in the interruption of an association between IRF-3 and IFNB promoter. Altogether, our results indicate that HBoV NP1 blocks IFN production through a unique mechanism. To our knowledge, this is the first study to investigate the modulation of innate immunity by HBoV. Our findings suggest a potential immune-evasion mechanism used by HBoV and provide a basis for better understanding HBoV pathogenesis.

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Human Bocavirus: Prevalence and Clinical Spectrum at a Children’s Hospital

Abstract: HBoV DNA is commonly present in children with upper and lower respiratory tract infections. The presence of a pertussis-like cough and diarrhea in association with HBoV infection merits further investigation.

Cited by 206 publications

References 14 publications

ELISAs using human bocavirus VP2 virus-like particles for detection of antibodies against HBoV

ELISAs using human bocavirus VP2 virus-like particles for detection of antibodies against HBoV

Human Bocavirus and KI/WU Polyomaviruses in Pediatric Intensive Care Patients

Human Bocavirus NP1 Inhibits IFN-β Production by Blocking Association of IFN Regulatory Factor 3 with IFNB Promoter

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