Human Bocavirus and KI/WU Polyomaviruses in Pediatric Intensive Care Patients

Pol, Alma C van de; Wolfs, Tom F. W.; Jansen, Nicolaas J. G.; Kimpen, Jan L. L.; Loon, Anton M. van; Rossen, John W. A.

doi:10.3201/eid1503.081203

Cited by 27 publications

(29 citation statements)

References 15 publications

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“…All samples were tested using real‐time PCR for the presence of respiratory viruses and bacteria including adenovirus (AdV), human bocavirus (hBoV), KI‐ and WU polyomaviruses (KIPyV and WUPyV), human metapneumovirus (hMPV), human rhinovirus (HRV), human coronaviruses (HCoV) (OC43, NL63, HKU and 229E), parainfluenza viruses (PIV), 1–4 influenza viruses A and B (InfA, InfB), respiratory syncytial virus (RSV), Legionella pneumophila, Mycoplasma pneumoniae , Chlamydophila psittaci, Chlamydophila pneumoniae , Coxiella burnetii and Streptococcus pneumoniae . Real‐time PCR procedures were performed as described in reference 16–22. Briefly, nucleic acids were extracted from the throat swabs with the MagNa pure LC using the total nucleic acid isolation kit system according to the manufacturer’s protocol (Roche Diagnostics, Basel, Switzerland).…”

Section: Methodsmentioning

confidence: 99%

Viral and bacterial aetiology of community‐acquired pneumonia in adults

Huijskens

Erkel

Palmen

et al. 2012

Influenza Resp Viruses

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Please cite this paper as: Huijskens et al. (2012) Viral and bacterial aetiology of community‐acquired pneumonia in adults. Influenza and Other Respiratory Viruses 7(4), 567–573. Background Modern molecular techniques reveal new information on the role of respiratory viruses in community‐acquired pneumonia. In this study, we tried to determine the prevalence of respiratory viruses and bacteria in patients with community‐acquired pneumonia who were admitted to the hospital. Methods Between April 2008 and April 2009, 408 adult patients (aged between 20 and 94 years) with community‐acquired pneumonia were tested for the presence of respiratory pathogens using bacterial cultures, real‐time PCR for viruses and bacteria, urinary antigen testing for Legionella and Pneumococci and serology for the presence of viral and bacterial pathogens. Results Pathogens were identified in 263 (64·5%) of the 408 patients. The most common single organisms in these 263 patients were Streptococcus pneumoniae (22·8%), Coxiella burnetii (6·8%) and influenza A virus (3·8%). Of the 263 patients detected with pathogens, 117 (44·5%) patients were positive for one or more viral pathogens. Of these 117 patients, 52 (44·4%) had no bacterial pathogen. Multiple virus infections (≥2) were found in 16 patients. Conclusion In conclusion, respiratory viruses are frequently found in patients with CAP and may therefore play an important role in the aetiology of this disease.

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Section: Methodsmentioning

confidence: 99%

Viral and bacterial aetiology of community‐acquired pneumonia in adults

Huijskens

Erkel

Palmen

et al. 2012

Influenza Resp Viruses

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“…Nonetheless, a few case reports describe HBoV as the cause of severe acute respiratory tract infection (SARI) in children requiring intensive care. Although the role of other pathogens was addressed, co-infections were not excluded using a structured method [3,[7][8][9][10][11][12][13][14]. Altogether, this has resulted in the on-going debate of whether HBoV as the single causative agent can cause disease.…”

Section: Introductionmentioning

confidence: 99%

Human bocavirus infection as a cause of severe acute respiratory tract infection in children

Moesker

Kampen

Eijk

et al. 2015

Clinical Microbiology and Infection

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In 2005 human bocavirus (HBoV) was discovered in respiratory tract samples of children. The role of HBoV as the single causative agent for respiratory tract infections remains unclear. Detection of HBoV in children with respiratory disease is frequently in combination with other viruses or bacteria. We set up an algorithm to study whether HBoV alone can cause severe acute respiratory tract infection (SARI) in children. The algorithm was developed to exclude cases with no other likely cause than HBoV for the need for admission to the paediatric intensive care unit (PICU) with SARI. We searched for other viruses by next-generation sequencing (NGS) in these cases and studied their HBoV viral loads. To benchmark our algorithm, the same was applied to respiratory syncytial virus (RSV)-positive patients. From our total group of 990 patients who tested positive for a respiratory virus by means of RT-PCR, HBoV and RSV were detected in 178 and 366 children admitted to our hospital. Forty-nine HBoV-positive patients and 72 RSV-positive patients were admitted to the PICU. We found seven single HBoV-infected cases with SARI admitted to PICU (7/49, 14%). They had no other detectable virus by NGS. They had much higher HBoV loads than other patients positive for HBoV. We identified 14 RSV-infected SARI patients with a single RSV infection (14/72, 19%). We conclude that our study provides strong support that HBoV can cause SARI in children in the absence of viral and bacterial co-infections.

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“…KIPyV has also been demonstrated from immunohistochemical staining of lung and splenic tissue from an HIV infected patient [Siebrasse et al, ]. Other studies, however, have not documented any increased association of the virus with symptomatic respiratory infections among subjects with and without immunocompromising conditions [Norja et al, ; Van de Pol et al, ].…”

Section: Discussionmentioning

confidence: 99%

Complete genome sequence of a KI polyomavirus isolated from an otherwise healthy child with severe lower respiratory tract infection

et al. 2016

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Unbiased, deep sequencing of a nasal specimen from an otherwise healthy 13-month-old boy hospitalized in intensive care revealed high gene expression and the complete genome of a novel isolate of KI polyomavirus (KIPyV). Further investigation detected minimal gene expression of additional viruses, suggesting that KIPyV was potentially the causal agent. Analysis of the complete genome of isolate NMKI001 revealed it is different from all previously reported genomes and contains two amino acid differences as compared to the closest virus isolate, Stockholm 380 (EF127908). J. Med. Virol. 89:926-930, 2017. © 2016 Wiley Periodicals, Inc.

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Human Bocavirus and KI/WU Polyomaviruses in Pediatric Intensive Care Patients

Cited by 27 publications

References 15 publications

Viral and bacterial aetiology of community‐acquired pneumonia in adults

Viral and bacterial aetiology of community‐acquired pneumonia in adults

Human bocavirus infection as a cause of severe acute respiratory tract infection in children

Complete genome sequence of a KI polyomavirus isolated from an otherwise healthy child with severe lower respiratory tract infection

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