Human Amniotic Epithelial Cells Possess Hepatocyte-like Characteristics and Functions

Takashima, Seiji; Ise, Hirohiko; Zhao, Peng; Akaike, Toshihiro; Nikaido, Toshio

doi:10.1247/csf.29.73

Cited by 133 publications

(90 citation statements)

References 41 publications

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“…In vitro studies have shown that hAEC produce albumin (Alb) and a-fetoprotein (AFP) , as well as performing other hepatic functions such as glycogen storage and expression of liverenriched transcription factors including hepatocyte nuclear factor (HNF) 3c and HNF4a, CCAAT/ enhancer-binding protein (CEBP) a and b and CYP450 enzymes (Davila et al 2004;Miki et al 2005;Takashima et al 2004).…”

Section: Human Amniotic Epithelial Cellsmentioning

confidence: 99%

Placenta-derived stem cells: new hope for cell therapy?

2008

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An urgent current need in regenerative medicine is that of identifying a plentiful, safe and ethically acceptable stem cell source for the development of therapeutic strategies to restore functionality in damaged or diseased organs and tissues. In this context, human term placenta represents a prime candidate, as it is available in nearly unlimited supply, is ethically problem-free and easily procured. Placental cells display differentiation capacity toward all three germ layers, while also displaying immunomodulatory effects, therefore supporting the possibility that they could be applied in an allogeneic transplantation setting. Although promising data have been reported to date, further study is required to fully characterize the differentiation potential of placenta-derived cells and to identify their possible clinical applications. Here, we provide a snapshot of current knowledge regarding the potential of cells from the amniotic membrane of human term placenta to address current shortcomings in the field of regenerative medicine.

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Section: Human Amniotic Epithelial Cellsmentioning

confidence: 99%

Placenta-derived stem cells: new hope for cell therapy?

2008

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“…Naïve hAEC display some relevant features of hepatic progenitors such as the lineage specification transcription factors FoxA2 and GATA-4 [22]. hAEC also display characteristic expression of mature hepatocyte markers such as albumin, a1-anti-trypsin, and OTC and also express various hepatocyte-related genes [22,23]. Upon differentiation, HLC derived from hAEC express mRNA of liver-enriched transcription factors such as C/EBP-a, CAR, RAR, and PXR [24], nuclear localization of the key transcription factor hepatocyte nuclear factor-4 alpha (HNF-4a), cytochrome P450 (CYP)1A1/2, CYP3A4, and CYP3A7 activity [20,21,25].…”

Section: Introductionmentioning

confidence: 99%

Hepatocyte-Like Cells Derived from Human Amniotic Epithelial Cells Can Be Encapsulated Without Loss of Viability or Function In Vitro

Vaghjiani

Vaithilingam

Saraswati

et al. 2014

Stem Cells and Development

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Placenta derived human amniotic epithelial cells (hAEC) are an attractive source of stem cells for the generation of hepatocyte-like cells (HLC) for therapeutic applications to treat liver diseases. During hAEC differentiation into HLC, they become increasingly immunogenic, which may result in immune cell-mediated rejection upon transplantation into allogeneic recipients. Placing cells within devices such as alginate microcapsules can prevent immune cell-mediated rejection. The aim of this study was to investigate the characteristics of HLC generated from hAEC and to examine the effects of encapsulation on HLC viability, gene expression, and function. hAEC were differentiated for 4 weeks and evaluated for hepatocyte-specific gene expression and function. Differentiated cells were encapsulated in barium alginate microcapsules and cultured for 7 days and the effect of encapsulation on cell viability, function, and hepatocyte related gene expression was determined. Differentiated cells performed key functions of hepatocytes including urea synthesis, drug-metabolizing cytochrome P450 (CYP)3A4 activity, indocyanine green (ICG) uptake, low-density lipoprotein (LDL) uptake, and exhibited glutathione antioxidant capacity. A number of hepatocyte-related genes involved in fat, cholesterol, bile acid synthesis, and xenobiotic metabolism were also expressed showing that the hAEC had differentiated into HLC. Upon encapsulation, the HLC remained viable for at least 7 days in culture, continued to express genes involved in fat, cholesterol, bile acid, and xenobiotic metabolism and had glutathione antioxidant capacity. CYP3A4 activity and urea synthesis by the encapsulated HLC were higher than that of monolayer HLC cultures. Functional HLC can be derived from hAEC, and HLC can be encapsulated within alginate microcapsules without losing viability or function in vitro.

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“…constructing a phylogenetic tree (Takashima et al 2004). Molecular phylogenetic tree was constructed by the NJ method based on 12S rRNA gene using the Kimura 2-parameter model (Figure 1).…”

Section: Resultsmentioning

confidence: 99%

Molecular evidence of two colour morphs of northern snakehead (Channa argus) based on mitochondrial 12S rRNA

Zhou

Xie

Wang

et al. 2017

Mitochondrial DNA Part B

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As an unclassified species, white-type Channa argus has existed for many years. In the present study, the relationships and genetic diversity in two colour morphs of northern snakehead were determined by the analysis of mitochondrial 12S rRNA gene, as well as other Channa species. The results of sequence analysis showed that the average genetic distance was 0.046 with the interspecies genetic distance ranging from 0.000 to 0.130; and the average interspecies genetic distance was estimated as 0.003 (range: 0.000-0.006). For the two colour morphs of Channa argus, the mean pair-wise genetic distance between them was also estimated as 0.003. Moreover, molecular phylogenetic tree was constructed using MEGA6.0, which showed that all the haploids gathered together as a branch and crossed each other. These results indicated that the white and biocolour-type Channa argus belonged to the same species, rather than subspecies at the molecular level, and the white-type Channa argus should be regard as an albino of biocolour type. ARTICLE HISTORY

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Human Amniotic Epithelial Cells Possess Hepatocyte-like Characteristics and Functions

Cited by 133 publications

References 41 publications

Placenta-derived stem cells: new hope for cell therapy?

Placenta-derived stem cells: new hope for cell therapy?

Hepatocyte-Like Cells Derived from Human Amniotic Epithelial Cells Can Be Encapsulated Without Loss of Viability or Function In Vitro

Molecular evidence of two colour morphs of northern snakehead (Channa argus) based on mitochondrial 12S rRNA

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