Human Amnion Mesenchyme Harbors Cells with Allogeneic T-Cell Suppression and Stimulation Capabilities

Magatti, Marta; Munari, Silvia; Vertua, Elsa; Gibelli, Lucia; Wengler, Georg S.; Parolini, Ornella

doi:10.1634/stemcells.2007-0491

Cited by 189 publications

(190 citation statements)

References 60 publications

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“…Our study also supports the sugthe minimal range of approximately 5 mM. This may be explained by the fact that human insulin was used to gestion that, in contrast to bone marrow-derived MSCs, this immunoregulation is less likely to be mediated by treat STZ-induced hyperglycemia in mice (29 (10), amniotic membrane mesenchymal (24) and epithelial cells (23), and amniotic fluid stem…”

Section: Clec-based Ex Vivo Gene Therapysupporting

confidence: 85%

Characterization of Human Umbilical Cord Lining-Derived Epithelial Cells and Transplantation Potential

et al. 2011

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In this study we describe the derivation and immunological characterization of a primary epithelial cell type from the human umbilical cord membrane. These cord lining epithelial cells (CLECs) expressed and/or secreted isoforms of the nonclassical human leukocyte antigen class I (HLA-1b) glycoproteins, HLA-G and E. Conditioned media from CLECs inhibited mitogen-stimulated T-lymphocyte responses, and in a mixed leukocyte reaction (MLR) assay, cocultured CLECs inhibited allogeneic responses with a concomitant reduction in proinflammatory cytokines. Using a transwell coculture system, it was demonstrated that these immunoregulatory effects were mediated by soluble factors secreted by CLECs, in a dose-dependent manner. Functional studies using HLA-G blocking antibody showed that the effects of CLEC-secreted products could be inhibited, thus demonstrating a significant and important role for soluble HLA-G. In vivo, we show that transplanted CLECs could be maintained for extended periods in immunocompetent mice where xenorejection rapidly destroyed primary keratinocytes, a control human epithelial cell type. Additionally, CLECs delayed the rejection of keratinocytes and extended their survival when cotransplanted, indicating an ability to protect adjacent human cell types that would otherwise be rejected if transplanted alone. We also show that CLECs transduced with a modified human proinsulin gene were transplanted intraperitoneally into streptozotocin (STZ)-induced diabetic mice, resulting in significantly lower levels of serum glucose compared to control mice. This study has characterized the immunological properties of CLECs and tested a potential therapeutic application in the treatment of a type 1 diabetes mouse model.

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Section: Clec-based Ex Vivo Gene Therapysupporting

confidence: 85%

Characterization of Human Umbilical Cord Lining-Derived Epithelial Cells and Transplantation Potential

et al. 2011

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“…In our study, we could demonstrate that bv-MSCs possess immunosuppressive properties similar to av-MSCs [37,38]. bv-MSCs inhibited the proliferation of activated PBMCs both in direct cell-to-cell contact as well as via soluble factors when separated by transwell chambers, suggesting that bv-MSCs are suitable for a use in cell transplantation settings.…”

Section: Discussionmentioning

confidence: 53%

“…Human-and mousederived av-MSCs improved bleomycin-induced lung fibrosis in a mouse model [36]. In vitro, av-MSCs suppressed Tlymphocyte proliferation [37,38], and prevented differentiation of monocytes into dendritic cells [39].…”

Section: Discussionmentioning

confidence: 97%

“…av-MSCs are also known to secrete a variety of immunomodulatory factors [13,37,[39][40][41][42]. The application of Cdm from av-MSCs reduced progression of lung fibrosis in a mouse model [43] and led to favorable clinical outcomes when treating tendon and ligament injuries in horses [44].…”

Section: Discussionmentioning

confidence: 99%

“…Human peripheral blood mononuclear cells (PBMCs) were obtained from heparinized whole-blood samples or buffy coats from healthy subjects using density gradient centrifugation (Axis-Shield) according to the protocol of Magatti et al [37].…”

Section: Lymphocyte Proliferation Testmentioning

confidence: 99%

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Placental Mesenchymal Stromal Cells Derived from Blood Vessels or Avascular Tissues: What Is the Better Choice to Support Endothelial Cell Function?

König

Weiss

Rossi

et al. 2015

Stem Cells and Development

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Mesenchymal stromal cells (MSCs) are promising tools for therapeutic revascularization of ischemic tissues and for support of vessel formation in engineered tissue constructs. Recently, we could show that avascular-derived MSCs from placental amnion release soluble factors that exhibit survival-enhancing effects on endothelial cells (ECs). We hypothesize that MSCs derived from placental blood vessels might have even more potent angiogenic effects. Therefore, we isolated and characterized MSCs from placental chorionic blood vessels (bv-MSCs) and tested their angiogenic potential in comparison to amnion-derived avascular MSCs (av-MSCs). bv-MSCs express a very similar surface marker profile compared with av-MSCs and could be differentiated toward the adipogenic and osteogenic lineages. bv-MSCs exert immunosuppressive properties on peripheral blood mononuclear cells, suggesting that they are suitable for cell transplantation settings. Conditioned medium (Cdm) from av-MSCs and bv-MSCs significantly enhanced EC viability, whereas only Cdm from bv-MSCs significantly increased EC migration and network formation (Matrigel assay). Angiogenesis array analysis of av-and bv-MSC-Cdm revealed a similar secretion pattern of angiogenic factors, including angiogenin, interleukins-6 and -8, and tissue inhibitors of matrix metalloproteinase-1 and 2. Enzyme-linked immunosorbent assay analysis showed that, in contrast to av-MSCs, bv-MSCs secreted vascular endothelial growth factor. In direct coculture with bv-MSCs, ECs showed a significantly increased formation of vessel-like structures compared with av-MSCs. With regard to therapeutic treatment, bv-MSCs and particularly their Cdm might be valuable to stimulate angiogenesis especially in ischemic tissues. av-MSCs and their Cdm could be beneficial in conditions when it is required to promote the survival and stabilization of blood vessels without the risk of unmeant angiogenesis.

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Isolation of Amniotic Mesenchymal Stem Cells

Marongiu

Gramignoli

Sun

et al. 2010

CP Stem Cell Biology

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Mesenchymal stem cells (MSCs) have the ability to differentiate into osteocytes, chondrocytes, and adipocytes and possess immunomodulatory properties. Amniotic membrane from human term placenta is a potential source of multipotent MSCs that could be useful for regenerative medicine. This unit describes a detailed and simple protocol for the isolation of amniotic mesenchymal cells. We also introduce a simple density separation technique for the purification of this cell type from possible contamination with amniotic epithelial cells.

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Human Amnion Mesenchyme Harbors Cells with Allogeneic T-Cell Suppression and Stimulation Capabilities

Cited by 189 publications

References 60 publications

Characterization of Human Umbilical Cord Lining-Derived Epithelial Cells and Transplantation Potential

Characterization of Human Umbilical Cord Lining-Derived Epithelial Cells and Transplantation Potential

Placental Mesenchymal Stromal Cells Derived from Blood Vessels or Avascular Tissues: What Is the Better Choice to Support Endothelial Cell Function?

Isolation of Amniotic Mesenchymal Stem Cells

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