Isolation of Amniotic Mesenchymal Stem Cells

Marongiu, Fabio; Gramignoli, Roberto; Sun, Qian; Tahan, Veysel; Miki, Toshio; Dorko, Kenneth; Ellis, Ewa; Strom, Stephen C.

doi:10.1002/9780470151808.sc01e05s12

Cited by 62 publications

(39 citation statements)

References 8 publications

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“…AM-MSCs were isolated according to Marongiu et al [23] and Kita et al [24] with modifications. Briefly, the AM was weighted, washed, and cut into pieces.…”

Section: Isolation Of Mscs From Uc Am and Bmmentioning

confidence: 99%

Mesenchymal Stromal Cell Characteristics Vary Depending on Their Origin

Wegmeyer

Bröske²,

Leddin³

et al. 2013

Stem Cells and Development

184

161

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Mesenchymal stromal cells (MSCs) are rare progenitor cells that can be isolated from various tissues. They exhibit multilineage differentiation potential, support regenerative processes, and interact with various immune cells. Therefore, MSCs represent a promising tool for regenerative medicine. However, source-dependent and donor-dependent differences of MSC properties, including implications on their clinical application are still largely unknown. We evaluated MSCs derived from perinatal tissues umbilical cord (UC) and amniotic membrane (AM) in comparison to adult MSCs from bone marrow (BM), which were used as gold standard. We found genetic background-independent differences between MSCs from UC and AM. While AM-and UC-MSCs were closer to each other than to BM-MSCs, they also exhibited differences between each other. AM-MSCs from different donors but not UC-MSCs displayed high interdonor variability. In addition, we show that although all MSCs expressed similar surface markers, MSC populations from UC and AM showed differential profiles of gene expression and paracrine factor secretion to BM-derived MSCs. Notably, pathway analysis of gene expression data revealed intriguing differences between MSCs suggesting that MSCs from UC and AM possess in general a higher potential of immunomodulatory capacity, whereas BM-MSCs showed a higher potential of supporting regenerative processes as exemplified by neuronal differentiation and development. These differences between perinatal and BM-derived MSCs may be relevant for clinical applications.

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“…AM-MSCs were isolated according to Marongiu et al [23] and Kita et al [24] with modifications. Briefly, the AM was weighted, washed, and cut into pieces.…”

Section: Isolation Of Mscs From Uc Am and Bmmentioning

confidence: 99%

Mesenchymal Stromal Cell Characteristics Vary Depending on Their Origin

Wegmeyer

Bröske²,

Leddin³

et al. 2013

Stem Cells and Development

184

161

View full text Add to dashboard Cite

show abstract

“…Cells from mesenchymal and epithelial amniotic layers, amniotic mesenchymal stromal and amniotic epithelial cells (AEC), respectively, can be obtained without any ethical concerns, in large amounts and with validated protocols [38], [39]. Amnion derived cells have been shown to maintain a remarkable plasticity and to possess a high self-renewing capacity [40]–[45].…”

Section: Introductionmentioning

confidence: 99%

Synthetic Bone Substitute Engineered with Amniotic Epithelial Cells Enhances Bone Regeneration after Maxillary Sinus Augmentation

et al. 2013

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BackgroundEvidence has been provided that a cell-based therapy combined with the use of bioactive materials may significantly improve bone regeneration prior to dental implant, although the identification of an ideal source of progenitor/stem cells remains to be determined.AimIn the present research, the bone regenerative property of an emerging source of progenitor cells, the amniotic epithelial cells (AEC), loaded on a calcium-phosphate synthetic bone substitute, made by direct rapid prototyping (rPT) technique, was evaluated in an animal study.Material And MethodsTwo blocks of synthetic bone substitute (∼0.14 cm3), alone or engineered with 1×106 ovine AEC (oAEC), were grafted bilaterally into maxillary sinuses of six adult sheep, an animal model chosen for its high translational value in dentistry. The sheep were then randomly divided into two groups and sacrificed at 45 and 90 days post implantation (p.i.). Tissue regeneration was evaluated in the sinus explants by micro-computer tomography (micro-CT), morphological, morphometric and biochemical analyses.Results And ConclusionsThe obtained data suggest that scaffold integration and bone deposition are positively influenced by allotransplantated oAEC. Sinus explants derived from sheep grafted with oAEC engineered scaffolds displayed a reduced fibrotic reaction, a limited inflammatory response and an accelerated process of angiogenesis. In addition, the presence of oAEC significantly stimulated osteogenesis either by enhancing bone deposition or making more extent the foci of bone nucleation. Besides the modulatory role played by oAEC in the crucial events successfully guiding tissue regeneration (angiogenesis, vascular endothelial growth factor expression and inflammation), data provided herein show that oAEC were also able to directly participate in the process of bone deposition, as suggested by the presence of oAEC entrapped within the newly deposited osteoid matrix and by their ability to switch-on the expression of a specific bone-related protein (osteocalcin, OCN) when transplanted into host tissues.

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“…We demonstrated that different inducing factors could affect the direction of differentiation of the AMSCs and that the AMSCs that originate from mesoblastema could differentiate into ectoderm and endoderm cell types. The autologous nature of these stem cells, together with their putative multi-potentiality and convenient procurement, makes them an excellent option for future tissue engineering and cell-based therapies (Marongiu et al 2010).…”

Section: Discussionmentioning

confidence: 99%

Research potential of multi-lineage chicken amniotic mesenchymal stem cells

Gao

Hua

et al. 2013

Biotechnic & Histochemistry

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Amniotic mesenchymal stem cells (AMSCs) express octamer binding transcription factor 4 (Oct-4), which is necessary for maintaining the undifferentiated state of pluripotent stem cells. AMSCs also express CD29, CD44 and vimentin, which are specific markers of mesenchymal cells. We studied the biological characteristics and potential for cell therapy of AMSCs derived from 8-day-old chicken embryos. We induced the AMSCs to differentiate into adipocytes, osteoblasts and myocardial cells and used immunofluorescence, reverse transcription-polymerase chain reaction (RT-PCR) assays to detect the expressions of specific markers of AMSCs and differentiated cells. To assess the differentiation capacity of AMSCs, passage four cells were induced to differentiate into adipocytes, osteoblasts and myocardial cells. These results suggested that AMSCs isolated from chicken embryos exhibited the characteristics of multipotent stem cells. AMSCs, therefore, may be potential candidates for cellular transplantation therapy and tissue engineering.

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Isolation of Amniotic Mesenchymal Stem Cells

Cited by 62 publications

References 8 publications

Mesenchymal Stromal Cell Characteristics Vary Depending on Their Origin

Mesenchymal Stromal Cell Characteristics Vary Depending on Their Origin

Synthetic Bone Substitute Engineered with Amniotic Epithelial Cells Enhances Bone Regeneration after Maxillary Sinus Augmentation

Research potential of multi-lineage chicken amniotic mesenchymal stem cells

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