2015
DOI: 10.1089/scd.2014.0115
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Placental Mesenchymal Stromal Cells Derived from Blood Vessels or Avascular Tissues: What Is the Better Choice to Support Endothelial Cell Function?

Abstract: Mesenchymal stromal cells (MSCs) are promising tools for therapeutic revascularization of ischemic tissues and for support of vessel formation in engineered tissue constructs. Recently, we could show that avascular-derived MSCs from placental amnion release soluble factors that exhibit survival-enhancing effects on endothelial cells (ECs). We hypothesize that MSCs derived from placental blood vessels might have even more potent angiogenic effects. Therefore, we isolated and characterized MSCs from placental ch… Show more

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Cited by 40 publications
(46 citation statements)
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References 71 publications
(88 reference statements)
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“…Many scholars have found that different types of cytotrophoblasts promote angiogenesis, which was similar to our results [6,[43][44][45][46]. For example, puri ed primary cytotrophoblasts were used by Knö er or Kato et al, the choriocarcinoma-derived BeWo cell line was used by Troja et al, and the normal human rst-trimester extravillous cytotrophoblasts (evCTBs)-derived HTR-8 cell line was used by Kalkunte or Das et al For hPDMSCs, our conclusion was also consistent with that of many studies; hPDMSCs obviously contribute to a series of cell activities to promote angiogenesis [9][10][11][12][13]15]. We further compared the effect of these two types of placental cells and found that primary placental cells derived from early pregnancy, whether CTBs or hPDMSCs, had more obvious effects in promoting angiogenesis than those of cells from middle and full-term pregnancy.…”
Section: Discussionsupporting
confidence: 87%
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“…Many scholars have found that different types of cytotrophoblasts promote angiogenesis, which was similar to our results [6,[43][44][45][46]. For example, puri ed primary cytotrophoblasts were used by Knö er or Kato et al, the choriocarcinoma-derived BeWo cell line was used by Troja et al, and the normal human rst-trimester extravillous cytotrophoblasts (evCTBs)-derived HTR-8 cell line was used by Kalkunte or Das et al For hPDMSCs, our conclusion was also consistent with that of many studies; hPDMSCs obviously contribute to a series of cell activities to promote angiogenesis [9][10][11][12][13]15]. We further compared the effect of these two types of placental cells and found that primary placental cells derived from early pregnancy, whether CTBs or hPDMSCs, had more obvious effects in promoting angiogenesis than those of cells from middle and full-term pregnancy.…”
Section: Discussionsupporting
confidence: 87%
“…Then, the medium was replaced with serum-free DMEM. At different time points (6,12,24,48, and 72 hours), the cell culture supernatant was collected and centrifuged at 3500 rpm for 20 minutes to remove detached cells and cellular debris. Then, the placental cell CM was ltered (0.22 μm), adjusted with serum-free medium to 10 ml/5× 10 7 cells, and frozen at -80 °C for future experiments.…”
Section: Conditioned Medium Preparation and Experimentsmentioning
confidence: 99%
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“…Although studies have demonstrated that MSC-conditioned media are able to promote EC viability, 16 we hypothesize that the successful establishment of prevascularized networks is co-dependent on an endothelial and supporting MSC co-culture differentiation. Different types of MSCs have been characterized 17, 18, 19 ; however, it remains unclear whether MSCs can retain their unique osteogenic potential during angiogenic differentiation.…”
Section: Introductionmentioning
confidence: 83%
“…These distinct in-vivo environments may affect the bioactivities of MSCs. Konig et al [51] demonstrated that placental MSCs isolated from blood vessels were better than those from avascular tissues on supporting endothelial cell functions. Jeon et al [52] reported that placenta-derived MSCs retained a higher therapeutic efficacy than BMSCs and AMSCs in the hind-limb ischemic disease model.…”
Section: Discussionmentioning
confidence: 99%