http://www.ijcmas.com/abstractview.php?ID=3029&amp;vol=6-7-2017&amp;SNo=54

Theodorea, Citra Fragrantia; Mashima, Izumi; Thaweboon, Boonyanit; Thaweboon, Sroisiri; Nakazawa, Fumio

doi:10.20546/ijcmas.2017.607.054

Cited by 6 publications

(10 citation statements)

References 29 publications

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“…It was previously reported that a higher number of anaerobic bacteria was detected on BHI agar in saliva from Thai children with poor oral hygiene than those with good and moderate oral hygiene 20 . This prior study demonstrated that oral Veillonella isolates were detected at a twofold higher frequency in the saliva of Thai children with poor rather than good or moderate oral hygiene 20 . Here, it was demonstrated that the number of anaerobic bacteria on BHI agar and Veillonella species on the selective medium increased in saliva of Japanese children with worsening oral hygiene status.…”

Section: Discussionmentioning

confidence: 91%

“…Then, 1,442 of 1,609 strains were detected by the one-step PCR method with the species-specific primer sets for V. atypica , V. denticariosi , V. dispar , V. parvula , V. rogosae , or V. tobetsuensis. They reported that V. rogosae was the predominant species detected in all groups 20 . These results of the previous and present studies indicate that V. rogosae is the predominant oral Veillonella species in the human saliva and tongue biofilm.…”

Section: Discussionmentioning

confidence: 98%

“…A previous study also showed that V. rogosae was the predominant Veillonella species in tongue biofilms of the children in Thailand 12 . Recently, Theodorea et al isolated 1,609 Veillonella strains from saliva samples of Thai children divided into three groups: good, moderate and poor oral hygiene status 20 . Then, 1,442 of 1,609 strains were detected by the one-step PCR method with the species-specific primer sets for V. atypica , V. denticariosi , V. dispar , V. parvula , V. rogosae , or V. tobetsuensis.…”

Section: Discussionmentioning

confidence: 99%

“…To resolve this problem, Mashima et al established a novel one-step PCR method with species-specific primer sets based on the variable region of the rpoB gene sequences of oral Veillonella species 12 . Additionally, 1,442 Veillonella strains isolated from the saliva of 107 Thai children were identified by this method as V. dispar , V. parvula , V. rogosae , V. atypica , V. denticariosi , and V. tobetsuensis in our previous study 20 . In that study, V. parvula was significantly more prevalent in the poor oral hygiene, and the detection rate of oral Veillonella species in the saliva was indicative of the oral hygiene status of Thai children 20 .…”

Section: Introductionmentioning

confidence: 99%

“…Additionally, 1,442 Veillonella strains isolated from the saliva of 107 Thai children were identified by this method as V. dispar , V. parvula , V. rogosae , V. atypica , V. denticariosi , and V. tobetsuensis in our previous study 20 . In that study, V. parvula was significantly more prevalent in the poor oral hygiene, and the detection rate of oral Veillonella species in the saliva was indicative of the oral hygiene status of Thai children 20 . Additionally, another study suggested that several novel Veillonella species may inhabit the human oral cavity 21 .…”

Section: Introductionmentioning

confidence: 99%

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Identification and phylogenetic analysis of oral Veillonella species isolated from the saliva of Japanese children

et al. 2019

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Background: As the most frequent infectious disease among children worldwide, dental caries have a strong relationship with oral hygiene status, specifically in the development of infection. However, the study regarding the identification and distribution of oral Veillonella are limited. The oral Veillonella community may affected by the differences in geographical location, age, diet, lifestyle, socio-economic status and oral hygiene status. Here, we studied the oral hygiene status by examining the composition and proportion of oral Veillonella species in saliva of Japanese children. Methods: Microbial samples collected from 15 Japanese children divided into three oral hygiene groups were cultured under anaerobic conditions after homogenization and dilution, and inoculated onto brain heart infusion and selective medium Veillonella agar. Genomic DNA was extracted from each isolate. Veillonella species were detected by one-step PCR using rpoB species-specific primers. To analyse the phylogenetic properties of the unknown Veillonella strains, PCR amplification and sequence analysis of rpoB were conducted for 10 representative strains. Results: Although V. rogosae was found as the predominant species among all groups, its prevalence was significantly lower in the children with poor oral hygiene than in those with good oral hygiene. V. parvula was the prevalent species in the poor oral hygiene group. Approximately 10% of the isolated Veillonella strains were not classified to any established species; the phylogenetic analysis showed that they were most closely related to V. infantium Conclusions: This study demonstrates that the composition and proportion of oral Veillonella species in the saliva of Japanese children is correlated with different oral hygiene status. Changes in detection ratios of V. parvula and V. rogosae can be useful indicators of oral hygiene status. Furthermore, new strains closely related to V. infantium were isolated from the saliva of Japanese children.

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Section: Discussionmentioning

confidence: 91%

Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Identification and phylogenetic analysis of oral Veillonella species isolated from the saliva of Japanese children

et al. 2019

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Establishment of a species-specific primer pair for detecting Veillonella infantium based on the 70 kDa heat shock protein gene dnaK

et al. 2018

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Veillonella nakazawae sp. nov., an anaerobic Gram-negative coccus isolated from the oral cavity of Japanese children

Mashima

Theodorea

Djais

et al. 2021

International Journal of Systematic and Evolutionary Microbiology

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Two strains of previously unknown Gram-negative cocci, T1-7T and S6-16, were isolated from the oral cavity of healthy Japanese children. The two strains showed atypical phenotypic characteristics of members of the genus Veillonella , including catalase production. Sequencing of their 16S rRNA genes confirmed that they belong to genus Veillonella . Under anaerobic conditions, the two strains produced acetic acid and propionic acid as metabolic end-products in a trypticase–yeast extract–haemin medium containing 1 % (w/v) glucose, 1 % (w/v) fructose and 1 % (v/v) sodium lactate. Comparative analysis of the 16S rRNA, dnaK, rpoB and gltA gene sequences revealed that the two strains are phylogenetically homogeneous and comprise a distinct, novel lineage within the genus Veillonella . The sequences from the two strains shared the highest similarity, at 99.9, 95.8, 96.9 and 96.7 %, using the partial 16S rRNA, dnaK, rpoB and gltA gene sequences, respectively, with the type strains of the two most closely related species, Veillonella dispar ATCC 17748T and Veillonella infantium JCM 31738T. Furthermore, strain T1-7T shared the highest average nucleotide identity (ANI) value (94.06 %) with type strain of the most closely related species, V. infantium . At the same time, strain T1-7T showed the highest digital DNA–DNA hybridization (dDDH) value (55.5 %) with the type strain of V. infantium . The two strains reported in this study were distinguished from the previously reported species from the genus Veillonella based on catalase production, partial dnaK, rpoB and gltA sequences, average ANI and dDDH values. Based on these observations, the two strains represent a novel species, for which the name Veillonella nakazawae sp. nov. is proposed. The type strain is T1-7T (JCM 33966T=CCUG 74597T).

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http://www.ijcmas.com/abstractview.php?ID=3029&vol=6-7-2017&SNo=54

Cited by 6 publications

References 29 publications

Identification and phylogenetic analysis of oral Veillonella species isolated from the saliva of Japanese children

Identification and phylogenetic analysis of oral Veillonella species isolated from the saliva of Japanese children

Establishment of a species-specific primer pair for detecting Veillonella infantium based on the 70 kDa heat shock protein gene dnaK

Veillonella nakazawae sp. nov., an anaerobic Gram-negative coccus isolated from the oral cavity of Japanese children

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http://www.ijcmas.com/abstractview.php?ID=3029&vol=6-7-2017&SNo=54

Cited by 6 publications

References 29 publications

Identification and phylogenetic analysis of oral Veillonella species isolated from the saliva of Japanese children

Identification and phylogenetic analysis of oral Veillonella species isolated from the saliva of Japanese children

Establishment of a species-specific primer pair for detecting Veillonella infantium based on the 70 kDa heat shock protein gene dnaK

Veillonella nakazawae sp. nov., an anaerobic Gram-negative coccus isolated from the oral cavity of Japanese children

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Establishment of a species-specific primer pair for detecting Veillonella infantium based on the 70 kDa heat shock protein gene dnaK