Establishment of a species-specific primer pair for detecting Veillonella infantium based on the 70 kDa heat shock protein gene dnaK

Mashima, Izumi; Djais, Ariadna Adisattya; Haase, Elaine M.; Scannapieco, Frank A.; Otomo, Maiko; Saitoh, Masato; Nakazawa, Fumio

doi:10.1016/j.anaerobe.2018.06.004

Cited by 4 publications

(4 citation statements)

References 24 publications

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“…In this study, there were several novelties, including the first time that the RT-qPCR machine was used to analyze oral Veillonella spp. with species-specific primer for Veillonella based on the rpoB and dnaK gene [ 11 , 27 , 28 ] from dental biofilm samples, and for the first time, an analysis of the relationship between the amount of oral Veillonella , stunting status, and oral hygiene index in Indonesia was conducted.…”

Section: Discussionmentioning

confidence: 99%

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Characterization of Oral Veillonella Species in Dental Biofilms in Healthy and Stunted Groups of Children Aged 6–7 Years in East Nusa Tenggara

Theodorea

Diven

Hendrawan

et al. 2022

IJERPH

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Impaired development that causes stunting is one of the most common health problems in Indonesia. In particular, the highest number of cases of stunting in Indonesia was reported in the East Nusa Tenggara (NTT) province. Previous studies have shown a tendency for deteriorating oral hygiene in children with a poor nutritional status. In addition, a higher proportion of oral Veillonella has been reported in children with poor oral hygiene. However, the relationship between populations of oral Veillonella and stunting has not been studied before. Therefore, this study aimed to analyze the oral Veillonella profile in the dental biofilms of healthy and stunted children aged 6–7 years. The participants were 60 elementary school students in the Nangapanda District, Ende, NTT, Indonesia. In this study, real-time polymerase chain reaction was used to examine dental biofilm samples from the healthy (n = 31) and stunted (n = 29) groups. The results revealed that seven oral Veillonella species were found in all groups. However, the number of four oral Veillonella species significantly differed between the healthy and stunted groups: V. denticariosi, V. infantium, V. rogosae, and V. tobetsuensis. This is the first study to demonstrate a potential association between oral Veillonella species and stunting in children.

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Section: Discussionmentioning

confidence: 99%

“…Specific primer sets of oral Veillonella species were used in this study for the identification of Veillonella species [ 27 , 28 ].…”

Section: Methodsmentioning

confidence: 99%

Characterization of Oral Veillonella Species in Dental Biofilms in Healthy and Stunted Groups of Children Aged 6–7 Years in East Nusa Tenggara

Theodorea

Diven

Hendrawan

et al. 2022

IJERPH

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“…Veillonella is strictly anaerobic and Gram‐negative. Veillonella can be found as a commensal microorganism in the lungs, vagina, mouth and GIT of humans 35 . Veillonella is common and considered mainly harmless 36 .…”

Section: Discussionmentioning

confidence: 99%

The effect of probiotic supplementation during pregnancy on the interaction network of vaginal microbiome

Liang

Tye

et al. 2020

J of Obstet and Gynaecol

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AimTo evaluate the effect of probiotic supplementation on the vaginal microbiome and provide the effective evidences for clinical management of pregnant women.MethodsA total of 28 healthy pregnant women at 32 weeks of gestation were enrolled. The women were divided randomly to the probiotic group where they were prescribed with 2 g combined probiotics daily (13 cases) during the third trimester of pregnancy or to the control group (15 cases) on a voluntary basis. Their vaginal samples were taken for analyzing microbiome with the 16S rDNA amplicon sequencing of V4 region.ResultsThere was no significant difference on the clinical characteristics between the probiotic and control groups. The complexity of vaginal microbial network increased from 32 weeks of gestation to antepartum. Lactobacillus was the dominant microbiota. The probiotic supplementation had no obvious influence on the structure of the vaginal microbiome, whereas the relationships of some pivotal vaginal microbiota at the genus level changed in the probiotic group.ConclusionThe vaginal microbiome varied during the third trimester of pregnancy. The features of the vaginal microbiota after probiotic supplementation had shifted and the interaction network had the tendency to be loose. The probiotic supplementation may be useful in regulating the interaction network of vaginal microbiome.

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“…Previous studies involving the molecular detection of Veillonella were mainly focused at the species level. For example, Rôças and Siqueira Jr (2006) reported the detection of V. parvula by nested PCR, and Mashima and Nakazawa reported the detection of V. tobetsuensis and V. infantium (Mashima and Nakazawa 2013;Mashima et al 2018a) by amplification of the DnaK gene. Although the specificity of species-level detection is high, the detection spectrum is narrow in clinical practice and difficult to apply to the detection of samples containing unknown pathogens.…”

Section: Introductionmentioning

confidence: 99%

Detecting and quantifying Veillonella by real-time quantitative PCR and droplet digital PCR

Ding,

Cui,

Zhang

et al. 2024

Appl Microbiol Biotechnol

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Veillonella spp. are Gram-negative opportunistic pathogens present in the respiratory, digestive, and reproductive tracts of mammals. An abnormal increase in Veillonella relative abundance in the body is closely associated with periodontitis, inflammatory bowel disease, urinary tract infections, and many other diseases. We designed a pair of primers and a probe based on the 16S rRNA gene sequences of Veillonella and conducted real-time quantitative PCR (qPCR) and droplet digital PCR (ddPCR) to quantify the abundance of Veillonella in fecal samples. These two methods were tested for specificity and sensitivity using simulated clinical samples. The sensitivity of qPCR was 100 copies/μL, allowing for the accurate detection of a wide range of Veillonella concentrations from 10 3 to 10 8 CFU/mL. The sensitivity of ddPCR was 11.3 copies/μL, only allowing for the accurate detection of Veillonella concentrations from 10 1 to 10 4 CFU/mL because of the limited number of droplets generated by ddPCR. ddPCR is therefore more suitable for the detection of low-abundance Veillonella samples. To characterize the validity of the assay system, clinical samples from children with inflammatory bowel disease were collected and analyzed, and the results were verified using isolation methods. We conclude that molecular assays targeting the 16S rRNA gene provides an important tool for the rapid diagnosis of chronic and infectious diseases caused by Veillonella and also supports the isolation and identification of Veillonella for research purposes. Key points• With suitable primer sets, the qPCR has a wider detection range than ddPCR.• ddPCR is suitable for the detection of low-abundance samples.• Methods successfully guided the isolation of Veillonella in clinical sample.

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Establishment of a species-specific primer pair for detecting Veillonella infantium based on the 70 kDa heat shock protein gene dnaK

Cited by 4 publications

References 24 publications

Characterization of Oral Veillonella Species in Dental Biofilms in Healthy and Stunted Groups of Children Aged 6–7 Years in East Nusa Tenggara

Characterization of Oral Veillonella Species in Dental Biofilms in Healthy and Stunted Groups of Children Aged 6–7 Years in East Nusa Tenggara

The effect of probiotic supplementation during pregnancy on the interaction network of vaginal microbiome

Detecting and quantifying Veillonella by real-time quantitative PCR and droplet digital PCR

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Establishment of a species-specific primer pair for detecting Veillonella infantium based on the 70 kDa heat shock protein gene dnaK

Cited by 4 publications

References 24 publications

Characterization of Oral Veillonella Species in Dental Biofilms in Healthy and Stunted Groups of Children Aged 6–7 Years in East Nusa Tenggara

Characterization of Oral Veillonella Species in Dental Biofilms in Healthy and Stunted Groups of Children Aged 6–7 Years in East Nusa Tenggara

The effect of probiotic supplementation during pregnancy on the interaction network of vaginal microbiome

Detecting and quantifying Veillonella by real-time quantitative PCR and droplet digital PCR

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Establishment of a species-specific primer pair for detecting Veillonella infantium based on the 70 kDa heat shock protein gene dnaK