Identification and phylogenetic analysis of oral Veillonella species isolated from the saliva of Japanese children

Abstract

Background: As the most frequent infectious disease among children worldwide, dental caries have a strong relationship with oral hygiene status, specifically in the development of infection. However, the study regarding the identification and distribution of oral Veillonella are limited. The oral Veillonella community may affected by the differences in geographical location, age, diet, lifestyle, socio-economic status and oral hygiene status. Here, we studied the oral hygiene status by examining the composition and proportion of oral Veillonella species in saliva of Japanese children. Methods: Microbial samples collected from 15 Japanese children divided into three oral hygiene groups were cultured under anaerobic conditions after homogenization and dilution, and inoculated onto brain heart infusion and selective medium Veillonella agar. Genomic DNA was extracted from each isolate. Veillonella species were detected by one-step PCR using rpoB species-specific primers. To analyse the phylogenetic properties of the unknown Veillonella strains, PCR amplification and sequence analysis of rpoB were conducted for 10 representative strains. Results: Although V. rogosae was found as the predominant species among all groups, its prevalence was significantly lower in the children with poor oral hygiene than in those with good oral hygiene. V. parvula was the prevalent species in the poor oral hygiene group. Approximately 10% of the isolated Veillonella strains were not classified to any established species; the phylogenetic analysis showed that they were most closely related to V. infantium Conclusions: This study demonstrates that the composition and proportion of oral Veillonella species in the saliva of Japanese children is correlated with different oral hygiene status. Changes in detection ratios of V. parvula and V. rogosae can be useful indicators of oral hygiene status. Furthermore, new strains closely related to V. infantium were isolated from the saliva of Japanese children.

Novel Isolation and Identification Methods for <i>Veillonella</i> Species Involved in Halitosis

“…In the present study, V. rogosae was predominant and was detected in the samples of all subjects, followed by V. tobetsuensis, V. atypica, and V. dispar. Beighton et al [26], Mashima et al [27], and Djais et al [28] also reported that V. rogosae, V. atypica, and V. dispar were the predominant Veillonella species in the human oral cavity, which is consistent with the present results. However, previous studies using VA reported that V. tobetsuensis was rarely detected in the human oral cavity [27] [28].…”

“…Previous studies [5] [6] [26] [27] [28] investigated the prevalence of oral Veillonella species using VA. In the present study using OVSM, oral Veillonella species were detected in all samples from healthy and halitosis subjects.…”

“…Previous studies [5] [6] [26] [27] [28] that examined the prevalence of oral Veillonella species used the well-known selective medium for Veillonella species, Veillonella agar (VA) [29]. In a pilot study, we attempted to detect oral Veillonella species in oral samples using VA.…”

Exclusively Breastfed Infant Microbiota Develops over Time and Is Associated with Human Milk Oligosaccharide Intakes

“…Additionally, we observed several individual HMO intakes supported the growth of infant oral bacteria such as Veillonella spp., S. mitis and S . salivarius, which are common oral cavity inhabitants [ 98 , 99 , 100 ]. Furthermore, we observed intakes of 3FL and LNFP II were negatively associated with abundance of S. epidermidis in the infant oral cavity.…”

Multimodal Data Integration Reveals Mode of Delivery and Snack Consumption Outrank Salivary Microbiome in Association With Caries Outcome in Thai Children

“…Studies have reported that age, geographic location, diet, and oral health behaviors influence the proportion of oral Veillonella species. One such study ( Djais et al., 2019 ) examined the proportion of oral Veillonella species in the saliva samples of Japanese children 4- to 14-years-old as a measure of their oral health status. Interestingly, the detection of V. rogosae declined with deteriorating oral hygiene status (49.1% in good hygiene vs. 44.4% in moderate hygiene vs. 34.1% in poor oral hygiene).…”