HSP70-mediated control of endothelial cell apoptosis during pre-eclampsia

Perumal, Ekambaram; Lavanya, S.

doi:10.1016/j.ejogrb.2011.01.026

Cited by 11 publications

(5 citation statements)

References 40 publications

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“…Hsp70 along with other pro-apoptotic proteins have been shown to mediate endothelial cell apoptosis in PE subjects [21], which is in agreement with the increased CRL1730 cell apoptosis upon culture with maternal sera obtained from PE patients. However, the apoptosis rate decreased over time.…”

Section: Discussionsupporting

confidence: 65%

Preeclampsia serum upregulates CD40/CD40L expression and induces apoptosis in human umbilical cord endothelial cells

Huang

Sui

et al. 2012

Reprod Biol Endocrinol

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BackgroundThe endothelial cell dysfunction observed in preeclampsia (PE) may be induced by CD40/CD40L signaling. This study investigated the role of CD40/CD40L in the pathogenesis of PE by comparing the effect of maternal serum obtained from healthy pregnant women and PE patients on HUVEC cell growth, apoptosis and CD40/CD40L expression.MethodsMaternal serum was obtained from 20 patients with PE (PE group) as well as 20 healthy pregnant women (control group). The human umbilical endothelial cell line, CRL1730, was cultured in the presence of maternal serum for 24, 48, and 72 h after which cell growth and apoptosis were assessed by MTT and flow cytometry analysis, respectively. CD40/CD40L expression was determined using flow cytometry and RT-PCR analyses.ResultsAs compared to CRL1730 cells treated with control sera, those treated with PE sera had altered morphology, decreased cell growth, increased apoptosis and greater CD40/CD40L protein and mRNA expression. Stimulation of CD40/CD40L protein and mRNA expression by PE sera was greatest at 24 h.ConclusionsPE sera may induce endothelial cell damage possibly through increased CD40/CD40L expression in early-onset PE. Further studies are necessary to determine the factor(s) in PE sera responsible for the observed changes in endothelial cell viability.

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Section: Discussionsupporting

confidence: 65%

Preeclampsia serum upregulates CD40/CD40L expression and induces apoptosis in human umbilical cord endothelial cells

Huang

Sui

et al. 2012

Reprod Biol Endocrinol

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“…Padmini et al [32] reported increased HSP 70 and HSF-1 in placentae from preeclampsia cases compare with uncomplicated pregnancies.…”

Section: Discussionmentioning

confidence: 98%

Heat Shock Protein 70 Expression Is Spatially Distributed in Human Placenta and Selectively Upregulated during Labor and Preeclampsia

2013

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Placental oxidative stress is a feature of both human labor and the pregnancy syndrome preeclampsia. Heat shock proteins (HSPs) can be induced in cells as a protective mechanism to cope with cellular stress. We hypothesized that HSP 70 would increase during labor and preeclampsia and that expression would vary in different placental zones. Samples were obtained from 12 sites within each placenta: 4 equally spaced apart pieces were sampled from the inner, middle and outer placental regions. Non-labor, labor and preeclampsia were studied. HSP 70 expression was investigated by Western blot analysis. HSP 70 protein expression was increased in the middle compared with the outer area (p = 0.03) in non-labor and in both the inner and middle areas compared with the outer area (p = 0.01 and p = 0.02 respectively) in labor. HSP 70 was increased in the preeclampsia non-labor group compared to the control non-labor group in the inner region (p = 0.003) and in the control labor group compared with the preeclampsia labor group at the middle area (p = 0.001). In conclusion HSP 70 is expressed in a spatial manner in the placenta. Changes in HSP 70 expression occur during labor and preeclampsia but at different zones within the placenta. The physiological and pathological significance of these remains to be elucidated but the results have important implications for how data obtained from studies in placental disease (and other organs) can be influenced by sampling methods.

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“…36 TRX-1 is localized in the cytosol of normal early and term placenta. [37][38][39] The expression of TRX-1 protein and mRNA levels were increased in PTD placenta in the present study (Fig. 2).…”

Section: Discussionsupporting

confidence: 48%