HSP70 in Gills and Hepatopancreas of Mangrove Crabs Ucides cordatus: Comparison between Contaminated and Pristine Environments

Abstract

This study analyzed field and acclimatized (7 days) mangrove Ucides cordatus crabs from polluted and unpolluted environments to compare their HSP70 levels. The animals were cryo-anesthetized and dissected. Gills (anterior and posterior) and hepatopancreas were collected to evaluate total proteins and HSP70 levels using ELISA (Enzyme-Linked Immunosorbent Assay) method. The acclimatized animals from polluted environments showed higher HSP70 levels in the hepatopancreas than field animals. Results showed higher HSP70 levels in laboratory animals from the polluted environment than in field animals in the posterior gills. The regulation to decrease the damage caused by the environment and the acclimatization process may not be sufficient to stabilize physiological responses, especially in animals from polluted environments.

Analysis of stress-related gene expression in lobster seeds (Panulirus sp.) post formalin stress test

“…HSP70 gene identification involves isolating total RNA from the hepatopancreas of lobster seeds. The use of hepatopancreas in gene expression analysis has been conducted in various crustacean studies [45,46,47,48] because of its vital role in metabolic processes [49] RNA concentration in lethargic seed conditions is 40,987±9,068 ng µL -1 with a ratio of 1.823±0.063; healthy seed samples had RNA concentrations of 133,570±14,515 ng µL -1 with a ratio of 2.063±0.037; and control lobsters exhibited RNA concentrations of 143,640±43,009 ng µL -1 with a ratio of 1.799±0.119. The obtained ratios indicated good fitness levels, with purity suggested in the range of 1.8-2.0, as recommended by [50].…”

The impact of chasing stress on the metabolism of the Atlantic Ghost Crab Ocypode quadrata (Fabricius, 1787)

“…Many types of environmental stresses such as alterations in temperature, pH, salinity; metal and xenobiotics exposure, and infections can upregulate HSP70 expression in crustaceans (Bao et al, 2014; Baringou et al, 2016; Chang, 2005; Chaurasia et al, 2016; Fernandes et al, 2018; Fu et al, 2013; Jin et al, 2022; Liu et al, 2018; Ortega & Vitorino, 2022; Qian et al, 2012). To our knowledge, only a few studies described upregulation of HSP70 1 h after the beginning of acute stress, such as when the shrimp L. vannamei , the mud crab Scylla paramamosain and the lobster Hommarus americanus were exposed to heat (Chang, 2005; Liu et al, 2018; Qian et al, 2012).…”

“…The heat shock response (HSR), characterized by the increase in the expression and activity of heat shock proteins (HSPs) or stress proteins, is another pathway triggered not only by temperature alterations but also by different types of environmental stresses, such as alterations in salinity and exposure to xenobiotics (Bao et al, 2014; Baringou et al, 2016; Chaurasia et al, 2016; Cottin et al, 2015; Fernandes et al, 2018; Ortega & Vitorino, 2022). HSPs comprise a family of highly conserved proteins ubiquitously present in all living organisms, from bacteria to mammals (Bao et al, 2014; Baringou et al, 2016; Chaurasia et al, 2016).…”