Analysis of stress-related gene expression in lobster seeds (Panulirus sp.) post formalin stress test

Abstract

Indonesia is the potential international-scale lobster economic activity due to the abundant supply of tropical lobster seeds Panulirus sp., however the lobster cultivation sector have still resulted in problems of low survival during the rearing period. This study aimed to analyze the expression levels of the HSP70 gene related to stress response in lobster seeds exposed to formalin dose of LC70 for 1 h. Seeds were obtained from catching lobsters at Ujung Genteng Beach, Sukabumi City, West Java Province, with an average weight of 0.0254±0.004 g and a length of 2.3314±0.1549 cm. The observation parameters tested were the gene expression and survival rates of the post-soaking lobsters. The results showed that formalin immersion at a dose of 220 ppm on juvenile-stage lobster seeds resulted in the death of 70% of the population and the ability to express the stress gene HSP70 at different fitness levels were different with the highest expression level found in fit condition lobster seeds was 2.949±0.420.