HRP immobilized microporous Poly(styrene-divinylbenzene-polyglutaraldehyde) monolith for forced flow injected phenol biosensing

Ozoner, Seyda Korkut; Keskinler, Bülent; Erhan, Elif

doi:10.1016/j.msec.2010.12.018

Cited by 14 publications

(6 citation statements)

References 25 publications

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“…The phenol biosensing system based on HRP immobilized on the surface of poly(styrene‐co‐divinylbenzene‐co‐polyglutaraldehyde) monolith prepared by means of high internal phase emulsion polymerization were developed by Ozoner with coauthors . At optimal conditions, corresponding to flow rate 1 mL/min, concentration of peroxide equal to 3 mM and phenol concentration up to 2 mM, the RSD value of 13 separate phenol injections was found to be 1.1%.…”

Section: Application Of Flow‐through Monolithic Bioreactorsmentioning

confidence: 99%

Flow‐through immobilized enzyme reactors based on monoliths: II. Kinetics study and application

Влах

Tennikova

2013

J of Separation Science

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In the last decade, the application of monolithic materials has rapidly expanded to the realization of flow-through bioconversion processes. Up to these days, different classes of enzymes such as hydrolases, lyases, and oxidoreductases have been immobilized on organic, inorganic, or hybrid monolithic materials to prepare the effective flow-through enzymes reactors for application in proteomics, biotechnology, pharmaceutics, organic synthesis, and biosensoring. Current review describes the results of kinetic study and specialties of flow-through immobilized enzyme reactors based on the existing monolithic materials.

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Section: Application Of Flow‐through Monolithic Bioreactorsmentioning

confidence: 99%

Flow‐through immobilized enzyme reactors based on monoliths: II. Kinetics study and application

Влах

Tennikova

2013

J of Separation Science

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“…This biocatalyst was successfully used to decontaminate artificially contaminated waters containing phenol, 2-methoxyphenol or 3-chlorophenol, with a removal of the contaminants ranging 75 to 100%. In another paper, the enzyme immobilized on microporous poly(styrene-divinylbenzenepolyglutaraldehyde) monoliths to develop a colorimetric detection for phenol biosensing [38]. The microarchitectured sstructure of the monolith improved mass-transferring for the enzymatic reaction due to the flow which forcibly had to go through the micropores.…”

Section: Immobilization Of Enzymes Via Covalent Attachmentmentioning

confidence: 99%

Immobilization of Proteins in Poly-Styrene-Divinylbenzene Matrices: Functional Properties and Applications

Rafael¹,

Hernández²,

Barbosa³

et al. 2015

COC

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Supports based on poly-styrene-divinylbenzene (PSD) are commercially available since a long time ago. However, they are not commonly used as enzyme immobilization matrices. The main reason for this lies in the negative effect of the very hydrophobic surface on enzyme stability that produces the instantaneous enzyme inactivation in many instances. However, they have recently regained some impact on enzyme immobilization. They are easy to modify, and have been prepared with different modifiers. We will pay special attention to the coating of these supports with ionic liquids, which permits to have the ionic liquid phase anchored to the solid and modulate the enzyme properties without risk of losing these expensive and potentially toxic compounds. Thus, this review will present the covalent or physical immobilization of enzymes on PSD supports, submitted to different modifications. Moreover, lipases immobilized via interfacial activation on some naked PSD supports have shown some unexpected improvement in their catalytic properties, with uses in reactions like hydrolysis, esterification or transesterification.

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“…Nevertheless, one successful example can be found in current literature. Recently, Ozoner et al reported the preparation of poly(styrene‐co‐divinylbenzene‐co‐polyglutaraldehyde) monolith using high internal phase emulsion polymerization mode . The introduced aldehyde groups allowed simple one‐step immobilization of horseradish peroxidase.…”

Section: Enzyme Immobilizationmentioning

confidence: 99%

Flow‐through immobilized enzyme reactors based on monoliths: I. Preparation of heterogeneous biocatalysts

Влах

Tennikova

2013

J of Separation Science

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The application of monoliths for realization of solid-phase biocatalytic processes was dramatically extended since the beginning of new century. Different enzyme immobilization techniques regarding these modern stationary phases have been developed, adapted, and optimized within last decade. The choice of enzyme immobilization method depends on material nature and monolith manufacturing. The present review collected, analyzed, and discussed the accessible published data on existing approaches and specialties of preparation of flow-through enzyme reactors based on monoliths.

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HRP immobilized microporous Poly(styrene-divinylbenzene-polyglutaraldehyde) monolith for forced flow injected phenol biosensing

Cited by 14 publications

References 25 publications

Flow‐through immobilized enzyme reactors based on monoliths: II. Kinetics study and application

Flow‐through immobilized enzyme reactors based on monoliths: II. Kinetics study and application

Immobilization of Proteins in Poly-Styrene-Divinylbenzene Matrices: Functional Properties and Applications

Flow‐through immobilized enzyme reactors based on monoliths: I. Preparation of heterogeneous biocatalysts

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