Flow‐through immobilized enzyme reactors based on monoliths: II. Kinetics study and application

Abstract

In the last decade, the application of monolithic materials has rapidly expanded to the realization of flow-through bioconversion processes. Up to these days, different classes of enzymes such as hydrolases, lyases, and oxidoreductases have been immobilized on organic, inorganic, or hybrid monolithic materials to prepare the effective flow-through enzymes reactors for application in proteomics, biotechnology, pharmaceutics, organic synthesis, and biosensoring. Current review describes the results of kinetic study and specialties of flow-through immobilized enzyme reactors based on the existing monolithic materials.

Preparation of high efficiency and low carry-over immobilized enzymatic reactor with methacrylic acid–silica hybrid monolith as matrix for on-line protein digestion

“…Compared to polymer monoliths, hybrid silica monoliths have higher binding capacity, and better permeability, mechanical strength and organic solvent tolerance [20][21][22], making them superior supports for enzyme immobilization. Ma et al [23] developed a novel immobilized trypsin reactor based on organic-inorganic hybrid silica monoliths.…”

Immobilized‐enzyme reactors integrated with capillary electrophoresis for pharmaceutical research

“…The monolith is prepared as a single piece of polymer with well‐defined channel‐like pores and surface chemistry (see Fig. for schematic depiction), thus variability of size, shape and immobilization approaches, high accessibility of the enzyme for substrates, and high stability are its inherent advantages . A number of thorough reviews concerning the applications of monoliths have been published, of which work by Svec and a recent contribution by Vlakh and Tennikova may be recommended for groups considering monolith applications.…”

Optimization of a nano‐enzymatic reactor for on‐line tryptic digestion of polypeptide conjugates by capillary electrophoresis

“…The products obtained are separated inside the same capillary under the application of electrical field for CE. The concept of nanoreactor was extensively used for on‐capillary derivatization for enhancing sensitivity and for the screening of large numbers of inhibitors .…”

“…Different mixing modes of reactants inside the capillary are referenced in the literature to help to routine experiments . Electrophoretically mediated microanalysis (EMMA) has been mainly used with drug metabolizing enzymes (moxidoreductases, kinases and transferases) . In EMMA format, buffers and reactants are hydrodynamically injected as successive plugs in the same capillary.…”