HPV 16 E7 inhibits OSCC cell proliferation, invasion, and metastasis by upregulating the expression of miR-20a

Hu, Jun; Ge, Weili; Xu, Junfeng

doi:10.1007/s13277-016-4817-4

Cited by 22 publications

(15 citation statements)

References 34 publications

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“…A recent deep sequencing study demonstrated that endogenous E6/E7 expression significantly affects the abundance of multiple intracellular miRNAs, such as miR‐17~92 cluster, in HPV‐positive cervical cancer cells, leading to the dysregulation of cell proliferation, senescence and apoptosis (Honegger et al ., ). miR‐20a and miR‐92a are the two important members of miR‐17~92 cluster and were found to be significantly up‐regulated in CINs and cancers in the present study, which was in line with other studies showing the overexpression of these two miRNAs in HPV‐infected cancer and cell lines (Hu et al ., ; Rao et al ., ; Wang et al ., ; Yu et al ., ). In addition, the expression of miR‐141 and miR‐210 was also reported to be regulated by HPV (Wang et al ., ).…”

Section: Resultsmentioning

confidence: 99%

Oncogenic microRNA signature for early diagnosis of cervical intraepithelial neoplasia and cancer

et al. 2018

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Cervical cancer is one of the leading causes of cancer death in women globally, despite the widespread use of cytology/human papillomavirus (HPV) screening. In the present study, we aimed to identify the potential role of microRNA (miRNA) as a diagnostic biomarker in the detection of cervical pre‐malignant lesions and cancer. In total, we recruited 582 patients with cervical diseases and 145 control individuals. The expression levels of six miRNAs (miR‐20a, miR‐92a, miR‐141, miR‐183*, miR‐210 and miR‐944) were found to be significantly up‐regulated in cervical cancer and pre‐malignant lesions compared to normal cervical samples, indicating that they are oncogenic miRNAs. Receiver operating characteristic curve analysis showed that these six miRNAs can be used to distinguish patients with cervical pre‐malignant lesions or cancer from normal individuals and they also had a good predictive performance, particularly in cervical lesions. Combined use of these six miRNAs further enhanced the diagnostic accuracy over any single miRNA marker, with an area under the curve of 0.998, 0.996 and 0.959, a diagnostic sensitivity of 97.9%, 97.2% and 91.4%, and a specificity of 98.6%, 96.6% and 87.6% for low‐grade lesions, high‐grade lesions and cancer, respectively. This six oncogenic miRNA signature may be suitable for use as diagnostic marker for cervical pre‐malignant lesions and cancer in the near future.

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Section: Resultsmentioning

confidence: 99%

Oncogenic microRNA signature for early diagnosis of cervical intraepithelial neoplasia and cancer

et al. 2018

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“…Our present study further supported these studies, although controversial data do exist; for example, Chang et al (27) profiled differentially expressed miRNAs in oral SCC cell lines and found that the miR-17-92 cluster (miR-17, miR-19b, miR-20a and miR-92a) was significantly downregulated in oral SCC cells (particularly, miR-17 and miR-20a), loss of which was associated with advanced oral squamous cancer tumor-node-metastasis (TNM) stage and lymph node metastasis. However, Hu et al (28) linked miR-20a expression to HPV16 E7, a risk factor for HNSCC, and revealed that miR-20a expression was significantly higher in oral SCC tissues compared to adjacent non-tumor tissues and that silencing of HPV-16 E7 expression downregulated the level of miR-20a in tumor cells and, in turn, reduced tumor cell proliferation, invasion and metastasis. Yu et al (29) revealed that when cytokine secretion in the cell microenvironment was changed, the role of miR-20a-5p in breast cancer cells was also altered.…”

Section: Discussionmentioning

confidence: 99%

Upregulated miR‑20a‑5p expression promotes proliferation and invasion of head and neck squamous cell carcinoma cells by targeting of TNFRSF21

Pang

Liu

et al. 2018

Oncol Rep

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MicroRNAs (miRNAs) play important roles in regulation of proliferation, migration, and invasion of head and neck squamous cell carcinoma (HNSCC). The present study assessed expression, functions and mechanisms of miR‑20a‑5p in the regulation of HNSCC cell proliferation, migration and invasion. miR‑20a‑5p expression in HNSCC cell lines and tissues was detected using qRT‑PCR, while miR‑20a‑5p mimics and inhibitor were transfected into HNSCC cells for assessment of the effects using different assays (CCK‑8, wound healing and Transwell assays) and expression of miR‑20a‑5p‑targeting genes (using western blot and luciferase reporter assays). The data revealed that miR‑20a‑5p was upregulated in both HNSCC tissues and metastatic HNSCC cells. Upregulated miR‑20a‑5p expression in HNSCC cells promoted tumor cell proliferation, migration and invasion capacities, but resulted in downregulation of TNFRSF21 expression and in turn upregulation of C‑C motif chemokine receptor 7 (CCR7) in HNSCC cells. Concordantly, knockdown of miR‑20a‑5p in HNSCC had the opposite results. In conclusion, miR‑20a‑5p functioned as an oncogene in HNSCC by downregulating TNFRSF21 and subsequently, upregulating CCR7 expression.

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“…Upregulation of miR-20a has been found to inhibit the proliferation, invasion and migration of cancer cells [ 16 , 50 , 51 ]. Whereby, overexpression of miR-20a has been reported to promote migration and invasion of various cancers [ 52 – 54 ].…”

Section: Discussionmentioning

confidence: 99%

MiR-20a, a novel promising biomarker to predict prognosis in human cancer: a meta-analysis

Huang

Peng

et al. 2018

BMC Cancer

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BackgroundRecently, microRNA-20a (miR-20a) has been reported to influence the clinical features and may have prognostic value in human cancers. The present meta-analysis assessed the prognostic role of miR-20a in various carcinomas.MethodsLiterature searches of seven electronic databases were performed for eligible articles of the prognostic role of miR-20a in human cancers. Hazard ratios (HR) for overall survival (OS), disease free survival (DFS), progression-free survival (PFS) as well as their 95% confidence intervals (95%CIs) were used to assess the influence of miR-20a expression on patient prognosis. Odds ratio (OR) and 95%CIs were applied to evaluate the correlation between miR-20a expression and clinicopathological characteristics.ResultsBased on the OS analyzed by log rank tests, there was a significant association between miR-20a levels and OS by fixed effects model. By subgroup analyses, the significance was also observed in the studies of specimen derived from blood and gastrointestinal cancer group. The independent prognostic role of miR-20a expression for the OS was observed significantly by fixed effects model. In addition, we observed significant association between miR-20a expression levels and DFS of log rank tests, DFS of cox regression. Significant relation of gender/differentiation and the expression level of miR-20a was identified.ConclusionsBase on the findings, the elevated miR-20a expression level is related to poor prognosis of gastrointestinal cancer patients. As for other types of carcinomas, the results are still not stable and more studies are required to further identify miR-20a prognostic values. In addition, miR-20a expression level is relatively higher in women than that in men, and increased miR-20a expression level is linked to poor tumor differentiation.

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HPV 16 E7 inhibits OSCC cell proliferation, invasion, and metastasis by upregulating the expression of miR-20a

Cited by 22 publications

References 34 publications

Oncogenic microRNA signature for early diagnosis of cervical intraepithelial neoplasia and cancer

Oncogenic microRNA signature for early diagnosis of cervical intraepithelial neoplasia and cancer

Upregulated miR‑20a‑5p expression promotes proliferation and invasion of head and neck squamous cell carcinoma cells by targeting of TNFRSF21

MiR-20a, a novel promising biomarker to predict prognosis in human cancer: a meta-analysis

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