HPTLC Fingerprints of Various Secondary Metabolites in the Traditional Medicinal Herb<i>Hypochaeris radicata</i>L.

Senguttuvan, Jamuna; Paulsamy, S.

doi:10.1155/2016/5429625

Cited by 40 publications

(30 citation statements)

References 28 publications

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“…CS2, used for the scanning of profiles of more polar compounds present in bee-pollen samples, gave chromatograms with good separation efficiency and low background noise. The applied mobile phase successfully separated medium and highly polar constituents (mainly the more polar phenolic acids such as chlorogenic, gallic and ellagic acids), and the different flavonoid aglycones (apigenin, quercetin, kaempferol, and their glycosides) [28,29]. Chromatographic profiles were rich in well defined, sharp zones which formed two dominant patterns, first with blue colored zones at R F values between 0.6 and 0.8 which are present in all samples and could be considered as a characteristic feature of Serbian bee-pollen, and second with orange bands that could be attributed to very polar glycosides at lower R F values, with profiles that depend on the samples and could be specific to certain botanical origins.…”

Section: Hptlc Fingerprint Of Phenolics In Bee-pollen Samplesmentioning

confidence: 99%

Phenolic Composition Influences the Health-Promoting Potential of Bee-Pollen

et al. 2019

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Information on compositional, nutritional and functional properties of bee-pollen, as a health-promoting food, is essential for defining its quality. Concerning the nutritional importance of phenolic compounds, the aim of this study was to determine the phenolic profile and antioxidant activity of twenty-four bee-pollen samples collected from different regions of Serbia. High-performance thin-layer chromatographic (HPTLC) fingerprinting was used for profiling of bee-pollen samples according to the botanical type. HPTLC hyphenated with image analysis and a pattern recognition technique confirmed the grouping of samples caused by the specific phenolic composition of pollens of different botanical origin. Flavonoid glycosides in bee-pollen samples were identified by applying ultra-high-performance liquid chromatography (UHPLC) coupled with linear ion trap-Orbitrap mass spectrometry (LTQ Orbitrap MS). Eight out of twenty-seven flavonol glycosides were identified in bee-pollen samples for the first time. All analyzed bee-pollen samples showed a high number of phenolic compounds which may have therapeutic potential.

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Section: Hptlc Fingerprint Of Phenolics In Bee-pollen Samplesmentioning

confidence: 99%

Phenolic Composition Influences the Health-Promoting Potential of Bee-Pollen

et al. 2019

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“…For method validation, ICH guidelines were followed . The comprehensive HPTLC analysis showed that the mobile phase consisting of Toluene: ethyl acetate: formic acid in the ratio of 3.5: 6:1(v/v/v) presented well resolved bands for methanolic extract of two plants along with the marker compounds, at R f 0.61 ± 0.02 for Quercetinand 0.07 ± 0.03 for Salicin . The chromatograms of standards (Quercetin and Salicin)and methanolic extract of different samplesare shown in Figure , The HPTLC chromatograms of Smilax china and Salix alba extracts with the reference compounds are shown in the Figure 2A, B, C, D, E and F.…”

Section: Resultsmentioning

confidence: 97%

Comparative evaluation of the liquid chromatographic methods for simultaneous analysis of quercetin and salicin in an anti‐psoriasis polyherbal formulation

Qadir

Aqil

Ali

et al. 2020

Separation Science Plus

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Smilax china L. and Salix alba are the medicinal plants of interest due to the presence of various therapeutic activities of these plants such as antiinflammatory, anti-diabetic, anti-psoriatic and digestive properties. Smilax china and Salix alba consists of bioactive marker Quercetin and Salicin, respectively. In the present study, a simple, rapid, reliable and accessible high-performance thin-layer chromatography method has been established for simultaneous quantification of quercetin and salicin. High-performance thin-layer chromatography fingerprinting was carried out on pre-coated Silica G60 F254 plates with toluene: ethyl acetate: formic acid (3.5:6.0:1.0 v/v/v) as mobile phase. The developed plates were scanned and quantified at two different λ max 380 and 275 nm. The drug samples were satisfactorily resolved with Rf 0.61 ± 0.02 for quercetin and 0.07 ± 0.03 for salicin. The method was validated for linearity, accuracy, precision, and specificity. The limits of detection and quantification for quercetin and salicin were 2.0 and 6.00 µg per band and 6.00 and 30.0 µg per band respectively. The developed high-performance thin-layer chromatography method can be implemented for the concurrent estimation of quercetin and salicin for routine quality control of herbal drugs containing these phytoconstituents.

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“…19 HPTLC fingerprinting is an accurate and precise instrumental technique to identify the phytoconstituents and adulterants present in medicinal plants. [20][21] HPTLC serves as an important highly efficacious technique to establish the appropriate quality control parameters for the herbal extracts. 22 The present investigation of HPTLC fingerprinting of SOE affirmed the presence of quercetin along with twelve other compounds.…”

Section: Discussionmentioning

confidence: 99%

Antidiabetic Potential and HPTLC Fingerprinting of Schleichera oleosa (Lour) Oken

Goswami¹,

Singh²

2019

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Introduction: Schleichera oleosa (Lour) Oken., also known as Lac tree, is a traditional plant used to treat different ailments since ancient time. The folkloric use of this plant as hypoglycemics is still un-revealed scientifically. So, the present study includes the in-vivo evaluation of hypoglycemic activity with HPTLC fingerprinting, qualitative and quantitative phytochemical screening of the extracts of the plant. Methods: Ethanolic extract of the plant (SOE) was subjected for phytochemical screening and HPTLC finger printing (using CAMAG LINOMAT 5 instrument). For the evaluation of hypoglycemic activity Streptozotocin (STZ) induced diabetic rats were treated with glibenclamide (0.5mg/kg body weight) and ethanolic extract (100 mg/kg and 200 mg/kg body weight) of the plant. Acute and chronic studies were performed for the evaluation of blood glucose levels. Results: The presence of alkaloids, tannins, phenolics and flavonoids were confirmed in the preliminary phytochemical screening. Quantitative screening of total tannins (6.15±4.20), total flavonoids (25.13±3.21) and total phenolic compounds (48.09±2.58) were reported. HPTLC fingerprinting analysis of SOE affirmed the presence of quercetin. Conclusion: The positive outcome was observed in the results of hypoglycemic activity, as all the treatments significantly decreased blood glucose level. During the study, SOE and glibenclamide maintained the body weight of the rats while diabetic control reduced the body weight by 11.33%. In support of its traditional use Schleichera oleosa (Lour) Oken. was proved to be a potent antidiabetic herbal plant.

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HPTLC Fingerprints of Various Secondary Metabolites in the Traditional Medicinal HerbHypochaeris radicataL.

Cited by 40 publications

References 28 publications

Phenolic Composition Influences the Health-Promoting Potential of Bee-Pollen

Phenolic Composition Influences the Health-Promoting Potential of Bee-Pollen

Comparative evaluation of the liquid chromatographic methods for simultaneous analysis of quercetin and salicin in an anti‐psoriasis polyherbal formulation

Antidiabetic Potential and HPTLC Fingerprinting of Schleichera oleosa (Lour) Oken

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