Comparative evaluation of the liquid chromatographic methods for simultaneous analysis of quercetin and salicin in an anti‐psoriasis polyherbal formulation

Abstract

Smilax china L. and Salix alba are the medicinal plants of interest due to the presence of various therapeutic activities of these plants such as antiinflammatory, anti-diabetic, anti-psoriatic and digestive properties. Smilax china and Salix alba consists of bioactive marker Quercetin and Salicin, respectively. In the present study, a simple, rapid, reliable and accessible high-performance thin-layer chromatography method has been established for simultaneous quantification of quercetin and salicin. High-performance thin-layer chromatography fingerprinting was carried out on pre-coated Silica G60 F254 plates with toluene: ethyl acetate: formic acid (3.5:6.0:1.0 v/v/v) as mobile phase. The developed plates were scanned and quantified at two different λ max 380 and 275 nm. The drug samples were satisfactorily resolved with Rf 0.61 ± 0.02 for quercetin and 0.07 ± 0.03 for salicin. The method was validated for linearity, accuracy, precision, and specificity. The limits of detection and quantification for quercetin and salicin were 2.0 and 6.00 µg per band and 6.00 and 30.0 µg per band respectively. The developed high-performance thin-layer chromatography method can be implemented for the concurrent estimation of quercetin and salicin for routine quality control of herbal drugs containing these phytoconstituents.

Heat Stress in Broiler Chickens and the Effect of Dietary Polyphenols, with Special Reference to Willow (Salix spp.) Bark Supplements - A Review

“…Having antipyretic and analgesic effects, salicin has been studied as treatment of fever and diseases (like arthritis) in humans [83]. Salicin is closely related to aspirin and has a very similar action in the body [96]. When ingested, salicin, the active glycoside of willow bark, is hydrolyzed in the intestine to saligenin.…”

“…Salicylic acid inhibits cyclooxygenase 1 (COX-1) and cyclooxygenase 2 (COX-2), the same enzymes targeted by synthetic non-steroidal anti-inflammatory drugs (NSAIDs) to alleviate pain and inflammation [137]. Because of this conversion process, white willow generally takes longer to act than aspirin, but the effects last for an extended period of time [96].…”