Phenolic Composition Influences the Health-Promoting Potential of Bee-Pollen

Mosić, Mirjana D.; Trifković, Jelena; Vovk, Irena; Gašić, Uroš; Tešić, Živoslav Lj.; Šikoparija, Branko; Milojković‐Opsenica, Dušanka

doi:10.3390/biom9120783

Cited by 42 publications

(37 citation statements)

References 43 publications

(53 reference statements)

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“…The lowest total flavonoid content was determined in honey (0.28-8.36 mg RUE/10 g). Bee pollen has been proven as the best source of flavonoid compounds by previous studies [41,42], thus obtained results coincide with these studies.…”

Section: Spectrophotometric Analysis Of Bee Pollen and Other Bee Prodsupporting

confidence: 86%

Comparison of Physicochemical Properties of Bee Pollen with Other Bee Products

et al. 2019

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The aim of this study was to compare the physichochemical composition of various bee products, namely, bee pollen, beebread, propolis, honey, and royal jelly. The samples (37 out of 53) were collected in Lithuania, several samples from other Europe countries (Italy, Denmark, Sweden, Slovakia, Poland, Spain, Republic of Malta, The Netherlands, Latvia, Ukraine) were used for comparison. Various quantities, such as pH, electrical conductivity, oxidation-reduction potential, NaCl content, refraction index, Brix value, total phenolic compound content, total flavonoid content and antiradical activity were measured. Together with the mentioned, the content of micro- and macroelements (As, Ba, Ca, Cd, Co, Cr, Cu, Fe, K, Mg, Mn, Na, Ni, P, Pb, Se, Sr, V and Zn), ultraviolet-visible spectroscopy absorption spectra were analysed. To our knowledge, the literature data about comprehensive comparison of various characteristics of bee products are scarce. Also, to the best of our knowledge, this is the first study revealing mineral content in Lithuanian bee pollen, beebread and royal jelly. The study exposed that bee pollen not only showed the highest values of pH, electrical conductivity and content of soluble solids, but also distinguished from the other samples by the highest flavonoid content (up to 48.3 mg/10 g), the absence of Cr, the presence of Co (0.011–0.100 mg/kg) and Sr (0.73–5.37 mg/kg) and the highest content of Ca (997–2455 mg/kg) and Mg (644–1004 mg/kg). Hierarchical clustering analysis was applied to group the tested samples according to the physicochemical analysis results and mineral content. The clustering analysis revealed that bee pollen formed separate group with the highest distance from the other samples in both cases.

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Section: Spectrophotometric Analysis Of Bee Pollen and Other Bee Prodsupporting

confidence: 86%

Comparison of Physicochemical Properties of Bee Pollen with Other Bee Products

et al. 2019

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“…As various polyphenols differ in their antioxidant effectiveness [ 46 ], the chromatographic profiles of the samples provided basic information about the antioxidant fraction composition and could be helpful as markers of developmental stage. In recent years, high-performance thin-layer chromatography has become an increasingly popular method of analyzing different compounds in bee products such as 10-hydroxy-2-decenoic acid [ 47 ], the sugar profile in honey [ 48 ], and the profile of phenolic compounds in bee pollen [ 49 ]. However, so far, this method has not been used for the analysis of drone brood.…”

Section: Discussionmentioning

confidence: 99%

Antioxidant Activity of Frozen and Freeze-Dried Drone Brood Homogenate Regarding the Stage of Larval Development

et al. 2021

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Drone brood is a little-known and poorly studied bee product used and valued in the treatment of many diseases, including male infertility and women’s menopausal disorders. The aim of this study was to evaluate the antioxidant activity of drone brood depending on the stage of larval development and the method of preservation. Aqueous and ethanolic homogenate extracts of drone brood were assayed for antioxidant activity (with the DPPH, FRAP, and ABTS methods), polyphenol, and flavonoid content. The extracts’ polyphenolic profiles were compared by the HPTLC method. Drone brood has been shown to be more active in the earlier stages of development (between days 7–11), with a decline in antioxidant activity in the later period (by the 14th day). The freeze-drying process did not cause significant changes in the antioxidant activity of brood preparations converted to dry mass. Based on the higher activity of the aqueous compared to 70% ethanolic extracts, it was shown that the dominant fraction of brood consisted of hydrophilic antioxidants. The results obtained with different methods were highly correlated, excluding those from the ABTS assay. The HPTLC method showed that the polyphenol fraction of drone brood homogenate consisted mainly of phenolic acids and flavonoids. It was shown that drone brood has valuable antioxidant properties that can be compared with royal jelly.

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“…The protein content and amino acid compositions of SBP have been reported previously (Taha et al, 2019). Beside nutrition, bee pollens are widely known as a natural product consisting of various biologically active substances which depend mainly on botanical origin (Dukhanina et al, 2006;Mosic et al, 2019).…”

Section: Discussionmentioning

confidence: 97%

Safflospermidines from the bee pollen of Helianthus annuus L. exhibit a higher in vitro antityrosinase activity than kojic acid

Khongkarat

Ramadhan

Phuwapraisirisan

et al. 2020

Heliyon

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Background: Ozone deterioration in the atmosphere has become a severe problem causing overexposure of ultraviolet light, which results in humans in melanin overproduction and can lead to many diseases, such as skin cancer and melasma, as well as undesirable esthetic appearances, such as freckles and hyperpigmentation. Although many compounds inhibit melanin overproduction, some of them are cytotoxic, unstable, and can cause skin irritation. Thus, searching for new natural compounds with antityrosinase activity and less/no side effects is still required. Here, bee pollen derived from sunflower (Helianthus annuus L.) was evaluated. Materials and methods: Sunflower bee pollen (SBP) was collected from Apis mellifera bees in Lopburi province, Thailand in 2017, extracted by methanol and sequentially partitioned with hexane and dichloromethane (DCM). The in vitro antityrosinase activity was evaluated using mushroom tyrosinase and the half maximal inhibitory concentration (IC 50 ) is reported. The antioxidation activity was determined using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay and reported as the half maximal effective concentration. Two pure compounds with antityrosinase activity were isolated by silica gel 60 column chromatography (SG 60 CC) and high performance liquid chromatography (HPLC), and their chemical structure deduced by Nuclear Magnetic Resonance (NMR) analysis. Results:The DCM partitioned extract of SBP (DCMSBP) had an antityrosinase activity (IC 50 , 159.4 μg/mL) and was fractionated by SG 60 CC, providing five fractions (DCMSBP1-5). The DCMSBP5 fraction was the most active (IC 50 ¼ 18.8 μg/mL) and further fractionation by HPLC gave two active fractions, revealed by NMR analysis to be safflospermidine A and B. Interestingly, both safflospermidine A and B had a higher antityrosinase activity (IC 50 of 13.8 and 31.8 μM, respectively) than kojic acid (IC 50 of 44.0 μM). However, fraction DCMSBP5 had no significant antioxidation activity, while fractions DCMSBP1-4 showed a lower antioxidation activity than ascorbic acid. Conclusion: Safflospermidine A and B are potential natural tyrosinase inhibitors.

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Phenolic Composition Influences the Health-Promoting Potential of Bee-Pollen

Cited by 42 publications

References 43 publications

Comparison of Physicochemical Properties of Bee Pollen with Other Bee Products

Comparison of Physicochemical Properties of Bee Pollen with Other Bee Products

Antioxidant Activity of Frozen and Freeze-Dried Drone Brood Homogenate Regarding the Stage of Larval Development

Safflospermidines from the bee pollen of Helianthus annuus L. exhibit a higher in vitro antityrosinase activity than kojic acid

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