HPTLC fingerprint profile, in vitro antioxidant and evaluation of antimicrobial compound produced from Brevibacillus brevis -EGS9 against multidrug resistant Staphylococcus aureus

Arumugam, T.; Kumar, Pankaj; Gopinath, Kannappan Panchamoorthy

doi:10.1016/j.micpath.2016.12.002

Cited by 7 publications

(7 citation statements)

References 14 publications

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“…High-performance thin-layer chromatography (HPTLC) is now emerging as an efficient, simple, specific, precise, and accurate as well as powerful analytical technique by which enormous samples can be analyzed at once. In the past few years, HPTLC technique has been proved to be a powerful technique for chemo-profiling in several medicinal plants [28,29]. In present study, mobile phase (S1) gives clear dense spots on TLC plate with R f of 0.62 for swertiamarin (II) and 0.8 for amarogentin (I) (Fig.…”

Section: Quantification Of Amarogentin Swertiamarin and Mangiferin Bmentioning

confidence: 50%

Optimization of salicylic acid and chitosan treatment for bitter secoiridoid and xanthone glycosides production in shoot cultures of Swertia paniculata using response surface methodology and artificial neural network

et al. 2020

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Background: In this study, response surface methodology (RSM) and artificial neural network (ANN) was used to construct the predicted models of linear, quadratic and interactive effects of two independent variables viz. salicylic acid (SA) and chitosan (CS) for the production of amarogentin (I), swertiamarin (II) and mangiferin (III) from shoot cultures of Swertia paniculata Wall. These compounds are the major therapeutic metabolites in the Swertia plant, which have significant role and demand in the pharmaceutical industries. Results: Present study highlighted that different concentrations of SA and CS elicitors substantially influenced the % yield of (I), (II) and (III) compounds in the shoot culture established on modified ½ MS medium (supplemented with 2.22 mM each of BA and KN and 2.54 mM NAA). In RSM, different response variables with linear, quadratic and 2 way interaction model were computed with five-factor-three level full factorial CCD. In ANN modelling, 13 runs of CCD matrix was divided into 3 subsets, with approximate 8:1:1 ratios to train, validate and test. The optimal enhancement of (I) (0.435%), (II) (4.987%) and (III) (4.357%) production was achieved in 14 days treatment in shoot cultures of S. paniculata elicited by 9 mM and 12 mg L − 1 concentrations (SA) and (CS). Conclusions: In optimization study, (I) show 0.170-0.435%; (II) display 1.020-4.987% and (III) upto 2.550-4.357% disparity with varied range of SA (1-20 mM) and CS (1-20 mg L − 1). Overall, optimization of elicitors to promote secoiridoid and xanthone glycoside production with ANN modeling (r 2 = 100%) offered more significant results as compared to RSM (r 2 = 99.8%).

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Section: Quantification Of Amarogentin Swertiamarin and Mangiferin Bmentioning

confidence: 50%

Optimization of salicylic acid and chitosan treatment for bitter secoiridoid and xanthone glycosides production in shoot cultures of Swertia paniculata using response surface methodology and artificial neural network

et al. 2020

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“…In the present investigation, the antibacterial activity of 25 Bacillus isolates against wide range of Gram-positive and Gram-negative bacteria were demonstrated. These isolates were identified based on their morphological, physical, and biochemical characteristics using Bergey"s manual and other scientific literature (Arbsuwan et al, 2014;Arumugam et al, 2017;Ramachandran et al, 2014;Malanicheva et al, 2012). It has been also observed that highest antibacterial activity was demonstrated against S. aureus and S. epidermis than E. coli.…”

Section: Discussionmentioning

confidence: 99%

“…subtilis, B. lichenoformis, B. coagulans, B. us megaterium, B. polymyxa, and Brevibacillus brevis. (Arbsuwan et al, 2014;Arumugam et al, 2017;Jianmei et al, 2015;Ramachandran et al, 2014;Malanicheva et al, 2012;Sumi et al, 2015).…”

Section: Identification Of Isolatesmentioning

confidence: 99%

Production of antimicrobial agents by Bacillus spp. isolated from Al-Khor coast soils, Qatar

Missoum¹,

Al‐Thani²

2017

Afr. J. Microbiol. Res.

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Bacilli are Gram-positive, sporulating bacteria that can be found in diverse habitats, but mostly in soil. Many recent studies showed the importance of their antimicrobial agents that mainly target other Grampositive bacteria. This study was conducted to assess the antibacterial activity of Al-Khor coastal soil Bacillus strains against four bacterial strains (Staphylococcus aureus, Staphylococcus epidermis, Escherichia coli, and Pseudomonas aeruginosa) by agar diffusion method as well as investigating best strain antibiotic production in liquid medium. The 25 isolated strains were identified using physical and biochemical tests. Results show that most strains possess significant antibacterial potential against S. aureus and S. epidermis, but however less toward E. coli. Strain 2B-1B optimum activity was achieved using Luria Bertani broth and at 35°C, with pH 9. Therefore, antimicrobial compounds from these strains can be good candidates for future antibiotics production. Further screening for antimicrobial agents should be carried out in search of novel therapeutic compounds.

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“…The quantitative similarity parameter (P i,FP ) was calculated using the peak area of the jth marker compound relative to that of the corresponding peak area in the RFP out of 90 common peaks at 278 nm, as shown in Eq. (12). The percentage of the Euclidean distance (d%) was adopted to estimate the proximity between P i and P i,FP , as shown in Eq.…”

Section: Fingerprint Evaluation By the Limited-ratio Quantified Fingementioning

confidence: 99%

“…Additionally, the techniques can be used to model pharmaceutical activities, such as the antioxidant and cytotoxic activities, in herbs . Among the current chromatography fingerprinting methods (e.g., HPLC with diode array detection (DAD), GC, high‐performance CE, TLC and GC–MS) that have been accepted as reliable methods for the identification and quantitation of HB constituents, HPLC is the most popular method, and it is widely used for fingerprint analyses due to its relatively high sensitivity, good reproducibility, and wide range of applicability . A fingerprint is a characteristic profile or pattern that provides information about the chemical composition of a sample and represents the selected HB and its raw materials .…”

Section: Introductionmentioning

confidence: 99%

Quantitative fingerprinting based on the limited‐ratio quantified fingerprint method for an overall quality consistency assessment and antioxidant activity determination of Lianqiao Baidu pills using HPLC with a diode array detector combined with chemometric methods

Chen

Sun

et al. 2017

J of Separation Science

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Lianqiao Baidu pills are widely used herbal medicinal preparation that were analyzed to develop a quality consistency technique. The characteristic fingerprints of 28 batches of Lianqiao Baidu pill samples were established at five wavelengths and simultaneously assessed by using a limited-ratio quantified fingerprint method using 15 marker compounds. The principal component analysis and fingerprinting results were compared, and the qualitative classification of the samples by principal component analysis agreed with their quantitative evaluation by the limited-ratio quantified fingerprint method. Furthermore, the antioxidant activities of the samples were surveyed and determined using a 2,2-diphenyl-1-picrylhydrazyl radical-scavenging approach. A relationship between the common peaks in the fingerprints and the antioxidant activities was established using a partial least squares model. The relationship can be used both to determine the antioxidant activities of the Lianqiao Baidu pill preparations in vitro and as a reference for the selection of active constituents for sample quality classification. The classification results for the samples based on principal component analysis agreed with the quantitative evaluation by the limited-ratio quantified fingerprint method, which demonstrated that the method can be applied to the holistic quality control of traditional Chinese medicine and herbal preparations.

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HPTLC fingerprint profile, in vitro antioxidant and evaluation of antimicrobial compound produced from Brevibacillus brevis -EGS9 against multidrug resistant Staphylococcus aureus

Cited by 7 publications

References 14 publications

Optimization of salicylic acid and chitosan treatment for bitter secoiridoid and xanthone glycosides production in shoot cultures of Swertia paniculata using response surface methodology and artificial neural network

Optimization of salicylic acid and chitosan treatment for bitter secoiridoid and xanthone glycosides production in shoot cultures of Swertia paniculata using response surface methodology and artificial neural network

Production of antimicrobial agents by Bacillus spp. isolated from Al-Khor coast soils, Qatar

Quantitative fingerprinting based on the limited‐ratio quantified fingerprint method for an overall quality consistency assessment and antioxidant activity determination of Lianqiao Baidu pills using HPLC with a diode array detector combined with chemometric methods

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