Quantitative fingerprinting based on the limited‐ratio quantified fingerprint method for an overall quality consistency assessment and antioxidant activity determination of Lianqiao Baidu pills using HPLC with a diode array detector combined with chemometric methods

Abstract

Lianqiao Baidu pills are widely used herbal medicinal preparation that were analyzed to develop a quality consistency technique. The characteristic fingerprints of 28 batches of Lianqiao Baidu pill samples were established at five wavelengths and simultaneously assessed by using a limited-ratio quantified fingerprint method using 15 marker compounds. The principal component analysis and fingerprinting results were compared, and the qualitative classification of the samples by principal component analysis agreed with their quantitative evaluation by the limited-ratio quantified fingerprint method. Furthermore, the antioxidant activities of the samples were surveyed and determined using a 2,2-diphenyl-1-picrylhydrazyl radical-scavenging approach. A relationship between the common peaks in the fingerprints and the antioxidant activities was established using a partial least squares model. The relationship can be used both to determine the antioxidant activities of the Lianqiao Baidu pill preparations in vitro and as a reference for the selection of active constituents for sample quality classification. The classification results for the samples based on principal component analysis agreed with the quantitative evaluation by the limited-ratio quantified fingerprint method, which demonstrated that the method can be applied to the holistic quality control of traditional Chinese medicine and herbal preparations.

Advances in Fingerprint Analysis for Standardization and Quality Control of Herbal Medicines

“…QFM typically uses 3 criteria to determine the quality grade of the sample: 1) qualitative similarity, which represents the similarity in the number and distribution of fingerprint peaks between the sample and reference, 2) quantitative similarity, which reflects the similarity in the overall content of the fingerprints, and 3) fingerprint variation coefficient, which represents the qualitative variation/dissimilarity of the fingerprints ( Liu et al, 2015 ). Based on how these 3 criteria are calculated, several QFMs have been reported such as the simple quantified ratio fingerprint method ( Liu et al, 2015 ), limited ratio quantified fingerprint method ( Chen et al, 2018 ), equal weight ratio quantitative fingerprint method ( Gong et al, 2021 ), linear quantitative profiling method ( Hou et al, 2019 ), averagely linear quantified fingerprint method ( Zhang et al, 2019 ), systematic quantified fingerprint method ( Lan et al, 2019 ), and average method of systematic quantified fingerprint method ( Wang et al, 2021b ).…”

“…The relationship between the CE fingerprints and other properties such as biological activities can also be further investigated using PLSR ( Chen et al, 2018 ; Hou et al, 2019 ). Other chemometric methods for classification such as PCA and HCA have also been explored on CE fingerprints ( Iino et al, 2012 ; Roblová et al, 2016 ; Hou et al, 2019 ).…”

Quantitative fingerprint and antioxidative properties of Artemisia argyi leaves combined with chemometrics

“…Approximately 0.5 ml solutions at different concentrations (0.2, 0.4, 0.8, 1.6, 2.0 mg/ml) was added to 1.5 ml DPPH solution and mixed evenly. The absorbance value was measured at 517 nm after storing the mixture at room temperature for 30 min in the dark, and quercetin was used as a positive control [31,32].…”

Simultaneous Determination of 12 Marker Components in Yeonkyopaedok-san Using HPLC–PDA and LC–MS/MS

“…It has anti-inflammatory effects (i.e., for treatment of colds, allergies, and dermatitis) and antiatherogenic effects [1][2][3]. Recently, Chen et al [4,5] reported a high-performance liquid chromatography (HPLC)-diode array detection and micellar electrokinetic chromatography assays for quality control of Lianqiao Baidu pills (LBP). In China, LBP is used for the treatment of diseases similar to those treated by YPS in Korea [4,5].…”

“…Recently, Chen et al [4,5] reported a high-performance liquid chromatography (HPLC)-diode array detection and micellar electrokinetic chromatography assays for quality control of Lianqiao Baidu pills (LBP). In China, LBP is used for the treatment of diseases similar to those treated by YPS in Korea [4,5]. However, LBP is a completely different herbal formulation, consisting of 19 herbal medicines [6].…”