HPLC quantification of seven quaternary benzo[c]phenanthridine alkaloids in six species of the family Papaveraceae

Jana, Snehasis; Bochořáková, Hana; Paulová, Hana; Musil, Pavel; Táborská, Eva

doi:10.1016/j.jpba.2007.02.005

Cited by 66 publications

(41 citation statements)

References 13 publications

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“…Fedde as was described previously (Táborská et al 1978;Dostal et al 1992). The alkaloids were at least 98% pure according to high-performance liquid chromatography (HPLC) analysis (Suchomelova et al 2007). …”

Section: Isolation and Identification Of The Selected Alkaloidsmentioning

confidence: 99%

“…Extracts obtained from these plants, especially M. cordata and S. canadensis, have been used in traditional medicine for their anti-inflammatory effects, particularly against plaque build-up and gingivitis (Šimánek et al 2003). Varying amounts of the major alkaloids sanguinarine and chelerythrine and the minor ones chelirubine, sanguirubine, chelilutine, sanguilutine, and macarpine have been found in these plants (Suchomelova et al 2007;Šimánek et al 2003;Slaninova et al 2014;Sebrlova et al 2015).…”

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confidence: 99%

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The capability of minor quaternary benzophenanthridine alkaloids to inhibit TNF-α secretion and cyclooxygenase activity

et al. 2017

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Quaternary benzophenanthridine alkaloids are known to have a wide range of biological effects, including antimicrobial, antifungal, anti-inflammatory, and antitumour activities. However, only sanguinarine and chelerythrine have been studied intensively. The aim of this study was to evaluate the anti-inflammatory potential of the five minor quaternary benzophenanthridine alkaloids sanguilutine, sanguirubine, chelirubine, chelilutine, and macarpine in vitro and to compare them with more thoroughly studied sanguinarine and chelerythrine. Before making cellbased assays, the cytotoxicity of the alkaloids was evaluated. The anti-inflammatory potential of the chosen alkaloids was evaluated as for their ability to modulate the lipopolysaccharideinduced secretion of tumour necrosis factor α (TNF-α) in the macrophage-like cell line THP-1. The cyclooxygenase (COX)-1 and COX-2 inhibitory activities were also measured. The results indicate that the presence of a methylenedioxy ring attached at carbon (C)7-C8 is important for reducing the secretion of TNF-α. Interestingly, this effect did not show a simple dependence on concentration. The selected alkaloids showed little or no anti-COX activity. The results obtained from the present experiments may provide additional information useful in understanding the structure-to-activity relationship of the quaternary benzophenanthridine alkaloids. The antiinflammatory potential and the cytotoxic effect are driven by the presence of a methylenedioxy ring attached at C7-C8 and C2-C3, respectively.

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Section: Isolation and Identification Of The Selected Alkaloidsmentioning

confidence: 99%

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confidence: 99%

The capability of minor quaternary benzophenanthridine alkaloids to inhibit TNF-α secretion and cyclooxygenase activity

et al. 2017

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“…Chelidonium alkaloids are usually separated by HPLC (Suchomelová et al, 2007). A good separation of chelidonine ( Figure 1) and other Chelidonium alkaloids was achieved by using a reversed phase (C18) stationary phase and for the mobile phase, a gradient of acetonitrile and deionised water acidified with formic acid.…”

Section: Hplc-dad Methods For Chelidonine In Chelidonium Majus Tincturementioning

confidence: 99%

Determination of shelf life of Chelidonium majus, Sambucus nigra, Thymus vulgaris and Thymus serpyllum herbal tinctures by various stability-indicating tests

Prosen

Pendry

2016

Phytochemistry Letters

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“…The total sanguinarine was measured with HPLC as described by Sucheomelova et al [30] with some modifications. One mg of dried plant extract was dissolved in one ml of phosphate buffer (0.01 M) solution and used for HPLC analysis.…”

Section: Isolation and Quantification Of Sanguinarinementioning

confidence: 99%

Antimicrobial Activity, Cytotoxicity and Phytochemical Analysis of Sanguinaria canadensis Native to South Dakota

NandaKafle¹,

Reese²,

Oda³

2017

OALib

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A methanolic extract of Sanguinaria canadensis rhizome contains a mixture of benzophenanthridine alkaloids with the major component sanguinarine. American Indians used this rhizome in treatment of rheumatism, asthma, bronchitis, lung ailments, fever and warts. Lethal diarrhea in neonatal and post weaned pigs due to enterotoxigenic Escherichia coli (ETEC) is a major problem among the swine producers of South Dakota region. The increasing resistance of ETEC towards synthetic antibiotics is a matter of concern. Our aims in this study were 1) to determine the minimum inhibitory concentration (MIC) of methanol extract of S. canadensis against ETEC, 2) to evaluate the cytotoxicity activity against porcine intestinal epithelial cell lines, and 3) to quantify the amount of sanguinarine in the rhizome of S. canadensis native to South Dakota. The MIC value was found to be 6.25 mg/mL against ETEC whereas the 100% inhibition of IPEC-J2 cells was found to be at 0.312 mg/mL. The total amount of alkaloids in this plant was found to be 1247 mg in 100 gm of fresh plant material whereas the amount of sangunarine was 559.64 mg in 100 gm of fresh plant material. The percentage of sangunarine in fresh plant material was 0.55% and in total alkaloid is 44.8%. Our results showed that S. canadensis has antibacterial effect against E. coli but it is toxic to IPECJ2 cells in vitro. It is important to carry out an in vivo animal study to confirm its further use as feed supplements. Subject AreasMicrobiology

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HPLC quantification of seven quaternary benzo[c]phenanthridine alkaloids in six species of the family Papaveraceae

Cited by 66 publications

References 13 publications

The capability of minor quaternary benzophenanthridine alkaloids to inhibit TNF-α secretion and cyclooxygenase activity

The capability of minor quaternary benzophenanthridine alkaloids to inhibit TNF-α secretion and cyclooxygenase activity

Determination of shelf life of Chelidonium majus, Sambucus nigra, Thymus vulgaris and Thymus serpyllum herbal tinctures by various stability-indicating tests

Antimicrobial Activity, Cytotoxicity and Phytochemical Analysis of Sanguinaria canadensis Native to South Dakota

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