Determination of shelf life of Chelidonium majus, Sambucus nigra, Thymus vulgaris and Thymus serpyllum herbal tinctures by various stability-indicating tests

Prosen, Helena; Pendry, Barbara

doi:10.1016/j.phytol.2016.05.012

Cited by 8 publications

(7 citation statements)

References 32 publications

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“…For many years, separation of C. majus extracts was mostly carried out in reversed phase (RP) system using long (150 or 250 mm) C18 columns with 4.6 mm of diameter and 5 μm of particle size (Niu and He, 1994 ; Petruczynik et al, 2002 ; Kursinszki et al, 2006 ; Borghini et al, 2015 ; Paulsen et al, 2015 ; Gañán et al, 2016 ). More recently, adsorbents with smaller particles (≤ 3 μm) or shorter columns (Prosen and Pendry, 2016 ; Seidler-Łozykowska et al, 2016 ) were also applied to achieve shorter separation and saving solvents. Mobile phases are usually composed of water and acetonitrile or/and methanol with various additives e.g., ammonium formate/acetate (Borghini et al, 2015 ; Seidler-Łozykowska et al, 2016 ), organic amines (triethyl-, tetrabutylamine) (Paulsen et al, 2015 ), ion-pair reagents (sodiumdodecylsulfate, alkylsulfonic acids) (Gañán et al, 2016 ).…”

Section: Methods For Analysis Of Active Components From Chementioning

confidence: 99%

Greater Celandine's Ups and Downs−21 Centuries of Medicinal Uses of Chelidonium majus From the Viewpoint of Today's Pharmacology

et al. 2018

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As antique as Dioscorides era are the first records on using Chelidonium as a remedy to several sicknesses. Inspired by the “signatura rerum” principle and an apparent ancient folk tradition, various indications were given, such as anti-jaundice and cholagogue, pain-relieving, and quite often mentioned—ophthalmological problems. Central and Eastern European folk medicine has always been using this herb extensively. In this region, the plant is known under many unique vernacular names, especially in Slavonic languages, associated or not with old Greek relation to “chelidon”—the swallow. Typically for Papaveroidae subfamily, yellow-colored latex is produced in abundance and leaks intensely upon injury. Major pharmacologically relevant components, most of which were first isolated over a century ago, are isoquinoline alkaloids—berberine, chelerythrine, chelidonine, coptisine, sanguinarine. Modern pharmacology took interest in this herb but it has not ended up in gaining an officially approved and evidence-based herbal medicine status. On the contrary, the number of relevant studies and publications tended to drop. Recently, some controversial reports and sometimes insufficiently proven studies appeared, suggesting anticancer properties. Anticancer potential was in line with anecdotical knowledge spread in East European countries, however, in the absence of directly-acting cytostatic compounds, some other mechanisms might be involved. Other properties that could boost the interest in this herb are antimicrobial and antiviral activities. Being a common synanthropic weed or ruderal plant, C. majus spreads in all temperate Eurasia and acclimates well to North America. Little is known about the natural variation of bioactive metabolites, including several aforementioned isoquinoline alkaloids. In this review, we put together older and recent literature data on phytochemistry, pharmacology, and clinical studies on C. majus aiming at a critical evaluation of state-of-the-art from the viewpoint of historical and folk indications. The controversies around this herb, the safety and drug quality issues and a prospective role in phytotherapy are discussed as well.

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Section: Methods For Analysis Of Active Components From Chementioning

confidence: 99%

Greater Celandine's Ups and Downs−21 Centuries of Medicinal Uses of Chelidonium majus From the Viewpoint of Today's Pharmacology

et al. 2018

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“…The herbal drug solution contained only rutin and isoquercitrin, whereas quercetin was also detected in the tincture, likely due to hydrolysis promoted by heating during the percolation extraction at 60°C. Prosen and Pendry [2] reported that quercetin was present in high concentrations in the commercial tincture, suggesting degradation of its glycosides, rutin and isoquercitrin.…”

mentioning

confidence: 99%

“…Stability testing of herbal preparations and herbal medicinal products (HMPs) has recently been recognized as essential for quality control to support the shelf lives of these products, and stability testing guidelines have been made official in many countries [1,2]. However, because of the complexity and different analytical behaviors of the components, their low concentrations, and a lack of information on each marker's contribution to the biological effect, studying the stability of herbal preparations and HMPs remains difficult.…”

mentioning

confidence: 99%

“…Prosen and Pendry [2] analyzed the stability of S. nigra flower tincture and reported decreases in rutin (40-50% after 3 months at 40°C and room temperature) and quercetin (30% and 80% at room temperature and 40°C, respectively, after 6 months), but found an increase in isoquercitrin (+128% at room temperature and +33.2% at 40°C). These authors discussed that rutin easily hydrolyzes to both isoquercitrin and quercetin.…”

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confidence: 99%

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Quantification of Sambucus nigra (Adoxaceae) Markers Related to Tincture Stability

Testoni

Souza

Krueger

et al. 2019

Natural Product Communications

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Stability tests are essential to assuring herbal derivative quality, especially because of the complexity of the herbal matrix. To monitor the stability of Sambucus nigra L. flower tincture, a practical liquid chromatography (LC)-UV method was developed and validated. Rutin, the flower's pharmacopeial marker, in addition to other markers, isoquercitrin and quercetin, were quantified. The last two represent the degradation products of glycosylated flavonoids. In addition, other chromatographic peaks exhibiting typical flavonoid UV absorption profiles (total flavonoids) were also quantified and expressed in rutin equivalents. The method was developed with a reverse phase C 18 column, using a gradient mobile phase at 0.7 mL min-1 at 30°C. The herbal drug and tincture obtained by percolation at 60 °C with ethanol 25% (v/v) were submitted to accelerated (40°C/75% RH, 2 months) and long duration (15-30°C, 6 months) studies. The LC-UV method was linear in the ranges of 1-200 µg mL-1 (rutin) and 1-100 µg mL-1 (isoquercitrin and quercetin), without interference of herbal matrix, and was precise (RSD ≤ 2.0% for intra and interday precision), selective, and robust (changes in mobile phase flow, pH and temperature). The tincture revealed the presence of quercetin, which was absent in the herbal drug, likely due to the hydrolysis process. After 2 months under accelerated study, the total flavonoids in the tincture decreased by approximately 20%, and after 6 months at room temperature, they decreased by approximately 30%. These results indicate the thermolability of the S. nigra tincture and the importance of monitoring phytomedicine stability.

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“…A lot of chromatographic systems for RP separation of isoquinoline alkaloids in C. majus were described in literature; however, most of them were more complicated [ 14 , 15 , 22 ] or did not provide sufficient separation for quantitative analysis [ 25 ]. Due to additional modification of endcapped octadecyl silica by isobutyl chains in the XB-C18 stationary phase, the interaction of the basic analyte with residual silanol decreased significantly.…”

Section: Resultsmentioning

confidence: 99%

Systematic Evaluation of Chromatographic Parameters for Isoquinoline Alkaloids on XB-C18 Core-Shell Column Using Different Mobile Phase Compositions

Sowa

Zielińska

Sawicki

et al. 2018

Journal of Analytical Methods in Chemistry

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Chelidonium majus L. is a rich source of isoquinoline alkaloids with confirmed anti-inflammatory, choleretic, spasmolytic, antitumor, and antimicrobial activities. However, their chromatographic analysis is difficult because they may exist both in charged and uncharged forms and may result in the irregular peak shape and the decrease in chromatographic system efficacy. In the present work, the separation of main C. majus alkaloids was optimized using a new-generation XB-C18 endcapped core-shell column dedicated for analysis of alkaline compounds. The influence of organic modifier concentration, addition of salts, and pH of eluents on chromatographic parameters such as retention, resolution, chromatographic plate numbers, and peak asymmetry was investigated. The results were applied to elaborate the optimal chromatographic system for simultaneous quantification of seven alkaloids from the root, herb, and fruit of C. majus.

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Determination of shelf life of Chelidonium majus, Sambucus nigra, Thymus vulgaris and Thymus serpyllum herbal tinctures by various stability-indicating tests

Cited by 8 publications

References 32 publications

Greater Celandine's Ups and Downs−21 Centuries of Medicinal Uses of Chelidonium majus From the Viewpoint of Today's Pharmacology

Greater Celandine's Ups and Downs−21 Centuries of Medicinal Uses of Chelidonium majus From the Viewpoint of Today's Pharmacology

Quantification of Sambucus nigra (Adoxaceae) Markers Related to Tincture Stability

Systematic Evaluation of Chromatographic Parameters for Isoquinoline Alkaloids on XB-C18 Core-Shell Column Using Different Mobile Phase Compositions

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