HPLC determination of pk_avalue of 10-hydroxy-2-decenoic acid

10-Hydroxy Decanoic Acid-Based Vesicles as a Novel Topical Delivery System: Would It Be a Better Platform Than Conventional Oleic Acid Ufasomes for Skin Cancer Treatment?

“…However, Group VI (prophylactic) showed the lowest MDA levels of 0.97 ± 0.08 µmol/mg of protein Figure 11 B, which were considered non-significantly different than that of the negative control ( p > 0.05). These results confirmed the effectiveness of Mag-HDA vesicles as a prophylactic treatment and endorsed the synergistic anti-cancer effect of HDA as reported [ 49 ].…”

“…Changing the fatty acid type from OA to HDA is accompanied by a significant reduction in the negative magnitudes of ZP values ( p < 0.05), where the respective average results are −80 and −55 mV, as revealed in Figure 2 C,D. This could be ascribed to the difference in pKa values of the two fatty acids where the majority of carboxyl groups of HDA, in the presence of Tris buffer, were neutralized, which decreased its electronegativity, compared to OA, where half ionization of its -COOH groups occurred under the same basic conditions [ 30 , 49 ].…”

“…Hence, the pH was adjusted at 8, a value equal to the pKa of OA, to ensure the ionization of half of the fatty acid carboxylic groups [ 25 , 27 , 28 ]. However, HDA-based vesicles were successfully prepared at the same pH producing stable vesicles although the HDA pKa value is equal to 5.5 [ 49 ]. This was in agreement with Novales et al, who reported the preparation of self-assembled vesicles composed of hydroxyl saturated fatty acids as 12-hydroxystearic acid and omega-hydroxy palmitic acid at pH (8.5–9) much higher than the fatty acids pKa value (=4.95).…”

“…Groups IV and V, treated with F-O2 and F-O3, respectively, showed a significant increase in GSH levels ( p < 0.05) compared to Group II (positive control), which proves their effectiveness in skin cancer treatment. These superior results of HDA-based formulations could be attributed to the synergistic antioxidant and antitumor effects of HDA [ 49 ].…”

Accurate Computation of Aqueous pKas of Biologically Relevant Organic Acids: Overcoming the Challenges Posed by Multiple Conformers, Tautomeric Equilibria, and Disparate Functional Groups with the Fully Black-Box pK-Yay Method

“…The reactions of acids with bases permeate throughout chemistry. , The resultant acid–base chemistry is very important in areas as diverse as enzyme driven biochemistry, − atmospheric chemistry, − materials science, − basic organic chemistry, − organometallic chemistry, − and nanochemistry. − Owing to both its ubiquity and significance, students learn about the concept of the acid dissociation constant ( K a ) and its logarithmic analogue p K a in various solvents, right from high school . Yet, both experimentally determining p K a values − and computing them via simulations continue to be actively pursued as key areas of contemporary research in chemistry. − …”