HPLC-DAD analysis of 15 monoterpene glycosides in oil peony seed cakes sourced from different cultivation areas in China

Liu, Pu; Yu, Zhang; Gao, Jiayu; Du, Mingzhu; Zhang, Kai; Zhang, Jianglei; Xue, Nan-chuan; Mi, Yan; Qu, Chun-xiao; Deng, Ruixue

doi:10.1016/j.indcrop.2018.03.033

Cited by 33 publications

(18 citation statements)

References 21 publications

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“…In the study, the developed method for simultaneous determination of seven oligostilbenes in P. ostii seed shell by HPLC‐DAD was validated using metrics including calibration linearity, limit of detection (LOD), limit of quantitation (LOQ), precision, repeatability and recovery . Linearity of standards was determined from the relationship between the peak area and concentration over the five concentration ranges of the working solutions.…”

Section: Methodsmentioning

confidence: 99%

“…The peony seed shell, accounting for more than 30% of the total mass, is one of the two most important by‐products in the preparation of peony seed oil. According to statistics, with the expanded cultivated area of tree peony in China, the annual seed yield reached 750000 tons . According to the earlier data, approximately more than 225000 tons of peony seed shells will be produced each year in the process of preparing peony seed oil.…”

Section: Introductionmentioning

confidence: 99%

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Profiling and simultaneous quantitative determination of oligostilbenes in Paeonia ostii seed shell from different geographical areas in China and their comparative evaluation

Zhang

Liu

Gao

et al. 2019

Phytochemical Analysis

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Introduction The Paeonia ostii T. Hong & J. X. Zhang seed shell, characterised by a high content of oligostilbenes, is one of the two most important by‐products in the preparation of seed oil. Oligostilbenes are considered characteristic constituents of the genus Paeonia, and can be used in fingerprinting to determine the geographical origin and the quality of raw materials. Objective To develop and optimise a simple and reproducible high‐performance liquid chromatography diode array detection (HPLC‐DAD) method for the simultaneous determination of seven oligostilbenes in P. ostii seed shell from different geographical areas, and to associate the cultivation area. Methodology A validated HPLC method coupled with a DAD detector was performed for the detection and determination of target compounds in the samples. Optimal chromatographic conditions were achieved using an Agilent Zorbax Eclipse SB‐AQ‐C18 column and a gradient elution with acetonitrile and potassium dihydrogen phosphate solution. Results The proposed quantitative method showed appropriate accuracy and precision, and was successfully applied to the routine analysis of seven oligostilbenes and the quality evaluation of 50 P. ostii seed shell samples. There were significant differences between the contents of the seven oligostilbenes in different samples (P < 0.01). Conclusion The results demonstrated that the oligostilbenes were main secondary metabolites in the P. ostii seed shells, and the content of seven components in P. ostii seed shells sourced from different cultivation areas in China was different.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Profiling and simultaneous quantitative determination of oligostilbenes in Paeonia ostii seed shell from different geographical areas in China and their comparative evaluation

Zhang

Liu

Gao

et al. 2019

Phytochemical Analysis

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“…An Agilent Technologies 1100 HPLC system (Agilent Technologies, US), consisting of a vacuum degasser, quaternary pump, autosampler, and a photodiode‐detector (DAD), was used for the simultaneous determination of fifteen monoterpene glycosides compounds in oil peony seed cake according to a previously described (Liu et al., , ). Chromatographic separations were carried out using a Zorbax C18‐AQ column (4.6 mm × 250 mm, 5 μm).…”

Section: Methodsmentioning

confidence: 99%

Optimization of Ultrasound‐Assisted Extraction of Monoterpene Glycoside from Oil Peony Seed Cake

Deng

Yang

Wang

et al. 2018

Journal of Food Science

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Ultrasound-assisted extraction (UAE) of total monoterpene glycosides extract (TMGE) from oil peony seed cakes was investigated. The extraction yield was optimized using response surface methodology (RSM). The chemical constituents of the monoterpene glycosides extract were isolated by repeated column chromatography, and the contents of the main isolated monoterpene glycosides in the oil peony seed cakes were determined by HPLC. The optimum conditions were as follows: a liquid-to-solid ratio of 27 mL/g, ultrasonic extraction time of 16 min, ultrasonic extraction temperature of 26°C, and ethanol concentration of 67%. Under these conditions, the extraction yield of TMGE was 10.24%. Twenty monoterpene glycosides were isolated from the oil peony seed cakes, and compounds 11-12, 16 and 20 showed strong inhibitory activities on NO production. TMGE from oil peony seed cakes can also to be used as promising immunosuppressive drug due to its high content of monoterpene glycosides and immune-inhibitory activity.Practical Aapplication: The peony seed oil was authorized as a new food by the Ministry of Health of the People's Republic of China. Peony seed cake is one of the most important by-products in the preparation of peony seed oil, and accounts for approximately 40% of the total mass of the peony seed. Total monoterpene glycosides are the main active ingredient of oil peony seed cake. This research has optimized the extraction conditions of total monoterpene glycoside from seeds cake of Paeonia ostii, which will provide useful reference information for further studies, and offer related industries with helpful guidance in practice.

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“…As a by‐product of Cortex Moutan, the oil content of peony seeds can be as high as 23.76%–27.06%. Peony seeds are rich in monoterpene glycosides, linoleic, and other unsaturated fatty acids, which makes it highly sought after (Deng et al., 2018; Liu et al., 2017; Liu, Zhang, Gao, Du, & Zhang, 2018, Liu, Zhang, Gao, Du, & Zhang, 2018; Liu, Zhang, Xu, Zhu, & Xu, 2018). The peony seed meal remaining after oil extraction has relatively few uses and is often used as feed for livestock and poultry breeding.…”

Section: Introductionmentioning

confidence: 99%

Study on immunoregulation function of peony seed proteolysis product in mice

Gong

Guo

2020

J Food Biochem

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To explore the immunoregulatory function of peony seed proteolysis product in mice, the protein from peony seed meal was extracted and hydrolyzed with bromelain. The peony seed proteolysis product was fed to mice at three different doses of 0.25, 0.5, and 1.0 g/kg for 21 days. The immunoregulation abilities of peony seed proteolysis product after each of these doses were evaluated in mice. Our results showed that all immune indices were higher in mice that had received a lavage with peony seed proteolysis product than in control mice. The immune indices of immune organs, delayed‐type hypersensitivity reaction (DTH), phagocytosis of peritoneal macrophages, serum hemolysin levels, lymphocyte proliferation (SI value), and levels of IFN‐γ and IL‐4 in the middle dose and high dose groups were significantly higher (p < .05) or extremely significant (p < .01) in comparison with the control group. These results indicate that the peony seed proteolysis product enhances immunological functions in mice. Practical applications Peony seed is rich in proteins and can be extracted and hydrolyzed using bromelain. The peony seed proteolysis product can enhance the nonspecific, humoral, and cellular immune responses. Thus, peony seed could be of potential value to obtain peony seed protein, which can be further developed and utilized in the manufacture of functional health products.

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HPLC-DAD analysis of 15 monoterpene glycosides in oil peony seed cakes sourced from different cultivation areas in China

Cited by 33 publications

References 21 publications

Profiling and simultaneous quantitative determination of oligostilbenes in Paeonia ostii seed shell from different geographical areas in China and their comparative evaluation

Profiling and simultaneous quantitative determination of oligostilbenes in Paeonia ostii seed shell from different geographical areas in China and their comparative evaluation

Optimization of Ultrasound‐Assisted Extraction of Monoterpene Glycoside from Oil Peony Seed Cake

Study on immunoregulation function of peony seed proteolysis product in mice

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