HPLC analysis of phospholipids in crude oil for evaluation of soybean deterioration

Mounts, T. L.; Nash, A. M.

doi:10.1007/bf02540486

Cited by 38 publications

(19 citation statements)

References 12 publications

(11 reference statements)

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“…Phospholipid components can be used to evaluate the quality of crude oil from oilseeds [4]. Indeed, phospholipids are essential components of cell membranes and play many roles such as maintaining homeostasis of cells, mediation of signal transduction and as ligands for receptors [5].…”

Section: Introductionmentioning

confidence: 99%

Triacylglycerols and Phospholipids Composition of Caper Seeds (Capparis spinosa)

Tlili

Trabelsi

Renaud³

et al. 2011

J Americ Oil Chem Soc

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The goal of this study is to evaluate for the first time the composition of triacylglycerols (TAG) using ESI-TOF-MS and phospholipids species using HPLC-ESI-TOF-MS of two Capparis spinosa seed oil populations. Results show that LOO, LOP, LLO, OOO, PLL and POO were the major molecular species of triacylglycerol detected in caper seeds; where L represents linoleic acid; O, oleic acid; and P, palmitic acid. The TAG composition was significantly different among the two C. spinosa populations. In Ghar el Melh population, LOO (15.7%) was detected as the dominant TAG molecular species, followed by LOP (13.2%), LLO (12.0%) and OOO (11.4%); while, the dominant fraction was LLO (14.2%) followed by LOO (14.1%), LOP (11.5%) and PLL (10.5%) in Chouigui samples. The major component in the phospholipids fraction was phosphatidylinositol (ca. 54-91%), followed by phosphatidylglycerol, phosphatidylethanolamine and phosphatidic acid. A variety of molecular species within each class were identified. The major component in all phospholipids species contains a C-18:1 lipid chain. C16:0/C18:2-PI (ca. 28-31%) was the most abundant PI. PG species were mainly C18:2/ C18:1-PG (25-32%). The major PE was C18:1/C18:1-PE (44-75%). The major PA species was C18:1/C18:1-PA (22-24%).

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Section: Introductionmentioning

confidence: 99%

Triacylglycerols and Phospholipids Composition of Caper Seeds (Capparis spinosa)

Tlili

Trabelsi

Renaud³

et al. 2011

J Americ Oil Chem Soc

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“…However, this technique could be applied only for qualitative purposes, because it is the amount of double bonds and not the number of molecules that is determined. Therefore, UV detection or derivatization methodology is usually associated with phosphorus quantification (1,2).…”

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confidence: 99%

Light‐scattering detection of phospholipids resolved by HPLC

Descalzo

Insani

Pensel

2003

Lipids

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An improved method for the analysis of phospholipids by normal-phase HPLC is described. Addition of methanol and acetonitrile to a gradient based on 2-propanol/hexane/water promoted a rapid separation of major classes of bovine surfactant phospholipids (PL) by using a conventional silica column. The use of an ELSD permitted an accurate analysis of a mixture of PL. Calibration curves were linear within the range of 5-40 microg with detection limits below 1 microg for PE and PC, and CV ranged from 0.6 to 9.6%. PL present in surfactant homogenates were separated by a solid-phase extraction (SPE) procedure before HPLC analysis. This methodology gave a recovery of 95% and combined SPE-HPLC and quantification of biological PL within a 30-min run. The use of ELSD detection of the eluted compounds was precise, linear, and sensitive.

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“…This fraction was separated without further purification or concentration. Phospholipid content in peanut seeds is low, therefore it is usually necessary to concentrate this fraction prior to HPLC analysis to obtain a useful chromatogram (14,15). This method eliminates preconcentration steps such as solid-phase extraction, open-column technology, and other previously used methodology for the extraction and analysis of phospholipids.…”

Section: Resultsmentioning

confidence: 99%

A solvent extractor system for the rapid extraction of lipids and trace bioactive micronutrients in oilseeds

Singleton

Stikeleather

1999

J Americ Oil Chem Soc

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A low‐cost laboratory extractor has been designed and constructed that selectively extracts polar and nonpolar components from oilseeds and other matrices. The extractor uses available high‐performance liquid chromatography laboratory equipment for pumping the solvent into the extractor. Pressure, temperature, and valving arrangements are automatically controlled by commercially available components. Advantages of this system include low initial investment, reduced solvent consumption, shorter extraction times, quantitative lipid recovery, use of multiple extraction solvents, and reduction in cost per sample. The method has broader applications that include extraction of trace components from a variety of matrices, for example, the extraction of pesticides from foods and polychlorobiphenyls from soil. Class separation of components from different matrices can be achieved easily by selection of solvents with the appropriate polarity characteristics. Very small samples can be extracted simply by changing cell size or by adding an inert material to the cell to fill the void volume. Analyte collection can be accomplished by collecting in a test tube with an appropriate solvent, or on a solid‐phase material. Optimization of extraction times, number of extractions, matrices, and solvent used is described. Neutral lipids were extracted from peanut meal in 70 min by the rapid extraction method compared to 1440 min required to extract the comparable amount of neutral lipids from a similar sample by the Soxhlet extraction method.

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HPLC analysis of phospholipids in crude oil for evaluation of soybean deterioration

Cited by 38 publications

References 12 publications

Triacylglycerols and Phospholipids Composition of Caper Seeds (Capparis spinosa)

Triacylglycerols and Phospholipids Composition of Caper Seeds (Capparis spinosa)

Light‐scattering detection of phospholipids resolved by HPLC

A solvent extractor system for the rapid extraction of lipids and trace bioactive micronutrients in oilseeds

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