A solvent extractor system for the rapid extraction of lipids and trace bioactive micronutrients in oilseeds

Abstract

A low‐cost laboratory extractor has been designed and constructed that selectively extracts polar and nonpolar components from oilseeds and other matrices. The extractor uses available high‐performance liquid chromatography laboratory equipment for pumping the solvent into the extractor. Pressure, temperature, and valving arrangements are automatically controlled by commercially available components. Advantages of this system include low initial investment, reduced solvent consumption, shorter extraction times, quantitative lipid recovery, use of multiple extraction solvents, and reduction in cost per sample. The method has broader applications that include extraction of trace components from a variety of matrices, for example, the extraction of pesticides from foods and polychlorobiphenyls from soil. Class separation of components from different matrices can be achieved easily by selection of solvents with the appropriate polarity characteristics. Very small samples can be extracted simply by changing cell size or by adding an inert material to the cell to fill the void volume. Analyte collection can be accomplished by collecting in a test tube with an appropriate solvent, or on a solid‐phase material. Optimization of extraction times, number of extractions, matrices, and solvent used is described. Neutral lipids were extracted from peanut meal in 70 min by the rapid extraction method compared to 1440 min required to extract the comparable amount of neutral lipids from a similar sample by the Soxhlet extraction method.

Optimisation of microwave‐assisted extraction for functional properties of Vitis coignetiae extract by response surface methodology

“…With regard to methods of extraction, pressing, steam distillation, reflux and supercritical fluid extraction have been used to extract effective components from medicinal herbs, but all these techniques have certain limitations in terms of extraction time, energy, solvent consumption and cost 12, 13. A microwave‐assisted process (MAP™ ) was developed as a new extraction technique 14.…”

The characteristics of a microwave extraction process used for saikosaponins from Bupleurum falcatum root

“…Pressing, steam distillation, reflux and supercritical fluid extraction have been used as methods of extracting effective components from medicinal herbs, but still have certain limitations in terms of extraction time, energy, solvent consumption and cost (Lopez‐Avila et al. , 1996; Singleton & Stikeleather, 1999). A microwave‐assisted process (MAP TM ) was developed as a new extraction technique (Paré et al.…”

LC‐electrospray ionization and LC‐FABMS study of flavonoid glycosides extracted from peanut meal

“…Peanuts were ground in a coffee grinder as finely as possible without producing a paste. The neutral lipid and polar fractions from peanuts were extracted using temperature and pressure (19). Approximately 10 g of peanuts was ground and placed in a high-pressure vessel.…”