The characteristics of a microwave extraction process used for saikosaponins from Bupleurum falcatum root

Abstract

The characteristics of a microwave-assisted process (MAP TM ) for extraction of saikosaponins from Bupleurum falcatum root were monitored, predicted and optimized through response surface methodology. The maximum yield of total extract was 27.3% when using 102.1 W of microwave power, 54.3% ethanol concentration and extraction for 8.34 min. The extraction patterns for the individual saikosaponins a, c and d showed apparent differences in their response surfaces depending on the process conditions. The maximized extractable contents of saikosaponins a, c and d were predicted, respectively, to be 4.54, 0.45 and 7.31 mg g )1 under the respective conditions of 46.4, 40.7 and 117.2 W of microwave power, 34.7, 47.1 and 67.9% ethanol concentration, and extraction times of 8.0, 7.9 and 3.4 min. A comparison between the predicted and observed values for optimizing the extraction conditions resulted in insignificant differences.

Optimization of supercritical fluid extraction of saikosaponins from Bupleurum falcatum with orthogonal array design

“…The yield obtained by SFE was lower than that obtained by the previously published method [30] due to the fact that saikosaponins are polarity substances. Hence, amounts of saikosaponins yielded by SFE may be always lower than that by using organic solvents.…”

“…At present, there have been many studies on B. falcatum, but most have focused on its pharmacology and clinical application and only few on the extraction technology of saikosaponins [30]. So the aim of this work was to optimize the extraction conditions of saikosaponins by a SFE system using an orthogonal test design.…”

Microwave-assisted extraction used for the isolation of total triterpenoid saponins from Ganoderma atrum

“…Recently, many research studies have been done in the developments of MAE methods for the extraction, such as extraction of taxanes from Taxus biomass (Mattina, Berger, & Denson, 1997), extraction of saikosaponins from Bupleurum falcatum root (Kwon, Choi, Chung, & Lee, 2006), extraction of anticancer drug camptothecin from Nothapodytes foetida (Fulzele & Satdive, 2005), extraction of essential oil from Cardamom (Marie, Jacqueline, Steven, Willem, & Farid, 2007), extraction of ginsenosides from ginseng root (Youn, Ming, & Yuan, 2003), extraction of azadirachtin-related limonoids from neem seed kernel (Dai, Yaylayan, Raghavan, & Parè, 1999), extraction of felodipine tablets (Eskilsson, Bjö rklund, Matheson, Karlsson, & Torstensson, 1999), extraction of glycyrrhizic acid from licorice roots (Pan, Liu, Jia, & Shu, 2000) and extraction of tea polyphenols and tea caffeine from green tea leaves (Pan, Niu, & Liu, 2002).…”

Development of gas chromatography–mass spectrometry with microwave distillation and simultaneous solid-phase microextraction for rapid determination of volatile constituents in ginger

“…Headspace solid-phase microextraction (HS-SPME) is a relatively new sampling and concentration technique for the extraction of plant essential oils [11][12][13][14][15]. In our previous studies [16][17][18][19][20][21][22], this technique has successfully been developed for the analysis of essential oils in TCMs.…”