Light‐scattering detection of phospholipids resolved by HPLC

Descalzo, Adriana María; Insani, E. M.; Pensel, Norma Ana

doi:10.1007/s11745-003-1154-1

Cited by 41 publications

(29 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Two phospholipids, phosphatidylcholine PC and sphingomyelin SM , were detected in all the samples. According to literature 10,35 , PC was the major phospholipid in all the three animals, accounting about the 60 of the total phospholipids content, because it is the key building blocks of membrane bilayers. In all samples, SM was present in lower amount compared to PC but it plays an important role in animals because it is a substitute for PC as a building block of membranes.…”

Section: Phospholipids Content Of Bone Marrowmentioning

confidence: 98%

See 1 more Smart Citation

Value-addition of Beef Meat By-products: Lipid Characterization by Chromatographic Techniques

Marzocchi

Pasini

Baldinelli³

et al. 2018

J. Oleo Sci.

View full text Add to dashboard Cite

Section: Phospholipids Content Of Bone Marrowmentioning

confidence: 98%

“…SFA were present in higher amount in YB sample, with an average content of 53.42 , followed with significant lower values by CO 45. 29 and CA 35,08 . On the other hand, MUFA were more abun- .…”

Section: Fatty Acid Composition Of By-productsmentioning

confidence: 99%

Value-addition of Beef Meat By-products: Lipid Characterization by Chromatographic Techniques

Marzocchi

Pasini

Baldinelli³

et al. 2018

J. Oleo Sci.

View full text Add to dashboard Cite

“…However, RI detectors have shown low sensitivity and a high degree of variability (depending upon the solvents used). UV detectors do not allow for the use of mobile phase that absorbs in UV range, such as chloroform that has been widely used in normal phase systems (Descalzo, Insani & Pensel, 2003). In order to avoid the undesirable baseline drift caused by solvent gradients during UV analyses, and to better facilitate effective separation and flexible gradient elution by high performance liquid chromatography, we used UHPLC coupled with Q Exactive Orbitrap mass spectrometry to identify all detected phospholipids, and HPLC combined with ELSD detector to quantify total PC content in various fruits and vegetables (especially due to the fact that phospholipid content for most of those samples are unavailable in the literature).…”

Section: Identification and Quantification Of Natural Lecithin Phosphmentioning

confidence: 99%

Identification and Quantification of Natural Lecithin Phospholipids and Their Residuals in Freeze-Dried and Drum-Dried Fruits and Vegetables by LC-MS and HPLC-ELSD

Xia

Gallegos-Peretz²,

Nemzer³

2017

JFR

View full text Add to dashboard Cite

Sunflower lecithin is commonly used as a food processing agent. In this study, residues of sunflower lecithin phospholipids in drum-dried fruits and vegetables were investigated. The contents of phosphatidylcholine and phosphatidylethanolamine were of interest due to their natural levels in fresh fruits and vegetables as well as their residues after the drum drying process. Identification of these compounds in freeze-dried and drum-dried fruits and vegetables was conducted by normal-phase and reverse-phase ultra-high-performance liquid chromatography (UPLC) coupled with Q Exactive Orbitrap electrospray mass spectrometry. Quantification of phosphatidylcholine in various fruits and vegetables was performed using normal-phase high-performance liquid chromatography with evaporative light scattering detector (ELSD). The quantification results from these various products demonstrate that use of de-oiled sunflower lecithin as a processing agent in the drum drying production process does not affect the quality of final drum-dried products.

show abstract

“…For the simultaneous detection of HPMC and DM in nanosuspension, we have selected ELSD as a detection method since both excipients are nonvolatile and lack UV chromophores (22,23). ELSD is much more sensitive over other techniques such as refractive index, and ELSD-based HPLC analytical methods can be easily transferred to liquid chromatographic mass spectroscopy (LCMS), as both methods employ compatible solvents (24).…”

Section: Introductionmentioning

confidence: 99%

Development of a Robust Method for Simultaneous Quantification of Polymer (HPMC) and Surfactant (Dodecyl β-D-Maltoside) in Nanosuspensions

Patel

Bummer

2015

AAPS PharmSciTech

View full text Add to dashboard Cite

Abstract. This report describes the development of a chromatographic method for the simultaneous quantification of a polymer, hydroxypropyl methylcellulose (HPMC), and a surfactant, dodecyl β-Dmaltoside (DM), that are commonly used in the physical stabilization of pharmaceutical formulations such as nanosuspensions and solid dispersions. These excipients are often challenging to quantify due to the lack of chromophores. A reverse phase size exclusion chromatography (SEC) with evaporative light scattering detector (ELSD) technique was utilized to develop an accurate and robust assay for the simultaneous quantification of HPMC and DM in a nanosuspension formulation. The statistical design of experiments was used to determine the influence of critical ELSD variables including temperature, pressure, and gain on accuracy, precision, and sensitivity of the assay. A robust design space was identified where it was determined that an increase in the temperature of the drift tube and gain of the instrument increased the accuracy and precision of the assay and a decrease in the nebulizer pressure value increased the sensitivity of the assay. In the optimized design space, response data showed that the assay could quantify HPMC and DM simultaneously with good accuracy, precision, and reproducibility. Overall, SEC-ELSD proved to be a powerful technique for the simultaneous quantification of HPMC and DM. This technique can be used to quantify the amount of HPMC and DM in nanosuspensions, which is critical to understanding their effects on the physical stability of nanosuspensions.KEY WORDS: design of experiments; dodecyl β-D-maltoside; evaporative light scattering detector; HPMC; nanosuspensions.

show abstract

Light‐scattering detection of phospholipids resolved by HPLC

Cited by 41 publications

References 35 publications

Value-addition of Beef Meat By-products: Lipid Characterization by Chromatographic Techniques

Value-addition of Beef Meat By-products: Lipid Characterization by Chromatographic Techniques

Identification and Quantification of Natural Lecithin Phospholipids and Their Residuals in Freeze-Dried and Drum-Dried Fruits and Vegetables by LC-MS and HPLC-ELSD

Development of a Robust Method for Simultaneous Quantification of Polymer (HPMC) and Surfactant (Dodecyl β-D-Maltoside) in Nanosuspensions

Contact Info

Product

Resources

About