Hot water leaves extracts of Zizyphus jujube exert antioxidative effects in vitro and cytotoxicity in human cancer cell lines

Abstract

Hot water (JLW) and 70% ethanol (JLE) extracts of jujube leaves were investigated for their antioxidant effect, cytotoxicity, and anticancer effects. The measured content of total polyphenolics in JLW and JLE was 200.4 mg g -1 and 234.6 mg g -1 , respectively. Extracts showed 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity of 91.8% (JLW) and 93.8% (JLE) at 1,000 g mL -1 . A radical scavenging activity of JLW and JLE was higher than that of Vitamin C (89.3%) (at 1,000 g mL -1). Results from the analysis of Super oxide dismutase (SOD)-like activity showed 33.9% for JLW and 34.8% for JLE (at 1,000 µg mL -1). Extracts showed no cytotoxicity in a normal human cell line, HEK293. In human cancer cells JLW extract showed higher antiproliferative activity than JLE extract. Inhibitory effect of JLW for human breast cancer cells (MCF 7), human stomach cancer cells (AGS), human lung cancer cells (A549) and human liver cancer cells (HepG2) growth was 16.9%, 61.3%, 57.7%, and 20.1% respectively, at 1,000 g mL -1. The results of our study suggest the potential use of Zizyphus jujube leaves extracts for use as an excellent antioxidant and antiproliferative substance.Additional key words: 1,1-diphenyl-2-picrylhydrazyl, antioxidant, antiproliferation, polyphenol Hort. Environ. Biotechnol. 52(6):635-640. 2011.

Influence of carboxy methyl cellulose (CMC) and pectin on rheological, physical stability and sensory properties of milk and concentrated jujuba mixture

“…Ten grams of jujuba fruit was washed, destined and ground by a household grinder and then was added to 100 ml boiling water for 5 min [30,34]. The solution was kept in the controlled conditions at 4°C for 72 h to obtain jujuba extract.…”

Can tree leaves be used as an alternative source of synthetic antioxidants? Use of jujube leaf extract in laying hens

“…Özgan ( 41 ) in a study investigating the possibilities of using grape seed oil in laying hens, reported that they thought that β-ovomucin in the structure of albumen was protected thanks to the antioxidant properties of this oil and accordingly, albumen height could increase. Many studies ( 13 , 14 , 42 , 43 ) have reported that jujube leaf extract has antioxidant properties. Based on this information, it can be said that JLE increased the albumen index and Haugh unit in the present study due to its antioxidant properties.…”

Antioxidant and Anti-inflammatory Activities of the Extract and Solvent Fractions of Hemistepta lyrata Bunge

“…이외에도 활성산소종은 체내 지질, 단백 질, DNA 등을 손상시키는 원인이 되며 세포 기능 억제 등 생 명체에 유해한 물질로 작용하여 인체에 다양한 질병을 유발하 는 것으로 보고되고 있다 (Cencioni et al, 2013;Mahakunakorn et al, 2004;Kalyanaraman, 2013). 또한 산화스트레스는 단백질 또는 염증 관련 사이토카인의 발현을 증가시켜 혈관 및 조직에 염증 반응을 유발하는 주요한 원인으로 작용하는 것으로 보고 되고 있다 (Kim and Son, 2011). 염증이란 외부로부터 들어온 항원(바이러스 등)에 대하여 염증 매개 물질들을 분비하는 등 생체 내 세포 및 조직 손상을 제거하기 위한 반응으로 주로 대 식세포가 관여하며, 이들 대식세포는 lipopolysaccharides(LPS) 등에 의해 활성화되어 nitric oxide(NO), prostaglandin E2(PGE2) *Corresponding author: Jong-Du Kim Tel : +82-2-3668-9864 E-mail : zhongdou@dongbang.ac.kr 등 염증 매개물질들이 분비되며, 이 중 NO는 세포독성, 혈관이 완, 세포 내 염증 반응 등의 작용을 하는 것으로 알려져 있다 (Ashley et al, 2012;Coleman, 2001) .…”