Antioxidant and Anti-inflammatory Activities of the Extract and Solvent Fractions of Hemistepta lyrata Bunge

Abstract

This study investigated antioxidant and anti-inflammatory in order to confirm the possibility of functional materials that can improve skin diseases using <i>Hemistepta lyrata</i> Bunge extracts and solvent fractions. As a result of measuring the total polyphenol content, the content was high in EtOAc fraction, and then the BuOH fraction is next. As a result of measuring DPPH free radical scavenging activities, it was confirmed that BuOH fractions had superior antioxidant activity compared to other solvent fractions. In particular, in the case of EtOAc fraction, it was confirmed that the antioxidant ability was excellent even at a low concentration compared to other fractions in ABTS and FRAP assay. Through this, it was confirmed that the higher the total polyphenol content, the higher the antioxidant effect. In addition, as a result of verifying the inflammatory efficacy of the extract and the solvent fraction using the J774 cell line, it was confirmed that the NO level decreased when the extract and the solvent fraction were treated. In particular, it is determined that the ethyl acetate fraction having a high total polyphenol content has superior antioxidant and anti-inflammatory activities and contains more active substances than other fractions. Therefore, in order to conduct additional research, it is determined that research on the compounds contained in the ethyl acetate fraction is necessary.