Host–parasite relationship of the geoduck Panopea abbreviata and the green alga Coccomyxa parasitica in the Argentinean Patagonian coast

Phylogenetic characterization and morphological and physiological aspects of a novel acidotolerant and halotolerant microalga Coccomyxa onubensis sp. nov. (Chlorophyta, Trebouxiophyceae)

“…Compared to salinity resistance of other Coccomyxa strains, C. onubensis might be considered as a salinity-robust species. Interestingly, all Coccomyxa strains found by Rodríguez et al (2008) and Vázquez et al (2010) as parasites of marine mussels belong to C. viridis, the most salinity-robust species in the study of Darienko et al (2015).…”

Phylogenetic and morphological characterization of the green alga infesting the horse mussel Modiolus modiolus from Vityaz Bay (Peter the Great Bay, Sea of Japan)

“…After removing the primer-binding sites from the sequences, we obtained a sequence with a length of 1521 nucleotides, identical for all seven samples. The sequence limited by the primers SSUF + SSUR (Vázquez et al, 2010) was completely identical to the sequence of 558 bases obtained in the first step. The sequence of the SSU rRNA gene of Coccomyxa sp., the green alga from M. modiolus, consisting of 1521 nucleotides was deposited in GenBank under accession number JQ717057.…”

“…This process is characterized by the formation of 2 N identical autospores or daughter cells regularly located within the mother cell wall. In M. modiolus tissues and in the tissues of several mussel species (Gray et al, 1999;Rodríguez et al, 2008;Crespo et al, 2009) and geoduck (Vázquez et al, 2010), the algae divided to form 2 to 4 daughter cells, whereas the alga isolated from the giant scallop P. magellanicus formed up to 16 autospores in culture (Stevenson and South, 1974).…”

“…In Bivalvia, infestation by green microalgae has been described for several species from Atlantic coastal waters: the horse mussel Modiolus modiolus (Wiborg, 1946, as cited in Mortensen et al (2005), the giant scallop Placopecten magellanicus (Naidu and South, 1970;Naidu, 1971;South, 1974, 1975), the mussels Mytilus edulis chilensis (Gray et al, 1999), M. edulis (Mortensen et al, 2005;Rodríguez et al, 2008) and M. galloprovincialis (Crespo et al, 2009), and the geoduck Panopea abbreviata (Vázquez et al, 2010). Recently, a microalga-infected horse mussel, M. modiolus, from the northwestern Pacific (Peter the Great Bay, Sea of Japan) was first reported (Syasina, 2011).…”

“…Thus, the morphology-based identification of Coccomyxa species is extremely difficult and necessitates the use of molecular phylogenetic analysis to identify these species (Rodríguez et al, 2008). To date, there are only two studies employing small subunit (SSU) rRNA sequence analysis to determine the phylogenetic position of the green microalgae infesting bivalve species from the North and South Atlantic, M. edulis (Rodríguez et al, 2008) and P. abbreviata (Vázquez et al, 2010).…”