Phylogenetic characterization and morphological and physiological aspects of a novel acidotolerant and halotolerant microalga Coccomyxa onubensis sp. nov. (Chlorophyta, Trebouxiophyceae)

Fuentes, Juan Luis; Huss, Volker A. R.; Montero, Zaida; Torronteras, Rafael; Cuaresma, María; Garbayo, Inés; Vílchez, Carlos

doi:10.1007/s10811-016-0887-3

Cited by 20 publications

(28 citation statements)

References 45 publications

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“…Coccomyxa onubensis ACCV1 was isolated from the acidic waters of the Tinto river, Huelva (Spain), phylogenetically characterized and deposited in the Experimental Phycology and Culture Collection of Algae at the University of Goettingen in Germany (SAG) with the stock number SAG 2510 [15]. According to the chemical composition of the acidic water in its natural environment, the medium used for maintaining axenic cultures of the microalga in the culture room was prepared at pH 2.5 by modifying the K9 medium described by Silverman and Lundgren [16].…”

Section: Microalga and Culture Mediummentioning

confidence: 99%

“…According to the chemical composition of the acidic water in its natural environment, the medium used for maintaining axenic cultures of the microalga in the culture room was prepared at pH 2.5 by modifying the K9 medium described by Silverman and Lundgren [16]. The culture medium was prepared in our laboratory with the following chemical composition: 22.67 mM K 2 SO 4 , 1.34 mM KCl, 2.87 mM K 2 HPO 4 , 4.31 mM MgCl 2 , 22.65 mM KNO 3 , 0.09 mM CaCl 2 , and 5 mL of Hutner's trace elements solution prepared in our laboratory as indicated in [15]. The microalgal cultures were grown in Erlenmeyer flasks (Fisher Scientific S.L., Madrid, Spain) in an algal room at 25 • C bubbled with air containing 2.5% (v/v) CO 2 and continuously illuminated by white fluorescent lamps.…”

Section: Microalga and Culture Mediummentioning

confidence: 99%

“…The 1 L of culture medium (NPK-medium) contained 0. Previous experiments at laboratory scale unveiled a more efficient use of urea by the microalga, as compared to other common nitrogen sources used to grow microalgae, including nitrate, nitrite, or ammonium [15,17]. Thus, the final medium was supplemented with 8 mM urea (Panreac Química Processes 2020, 8, 324 3 of 10 SLU, Barcelona, Spain) which also provides carbon to the microalga, 100 µM FeCl 3 , and pH was adjusted to 2.5.…”

Section: Microalga and Culture Mediummentioning

confidence: 99%

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Outdoor Large-Scale Cultivation of the Acidophilic Microalga Coccomyxa onubensis in a Vertical Close Photobioreactor for Lutein Production

et al. 2020

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The large-scale biomass production is an essential step in the biotechnological applications of microalgae. Coccomyxa onubensis is an acidophilic microalga isolated from the highly acidic waters of Río Tinto (province of Huelva, Spain) and has been shown to accumulate a high concentration of lutein (9.7 mg g−1dw), a valuable antioxidant, when grown at laboratory-scale. A productivity of 0.14 g L−1 d−1 was obtained by growing the microalga under outdoor conditions in an 800 L tubular photobioreactor. The results show a stable biomass production for at least one month and with a lutein content of 10 mg g−1dw, at pH values in the range 2.5–3.0 and temperature in the range 10–25 °C. Culture density, temperature, and CO2 availability in highly acidic medium are rate-limiting conditions for the microalgal growth. These aspects are discussed in this paper in order to improve the outdoor culture conditions for competitive applications of C. onubensis.

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Section: Microalga and Culture Mediummentioning

confidence: 99%

Section: Microalga and Culture Mediummentioning

confidence: 99%

Section: Microalga and Culture Mediummentioning

confidence: 99%

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Outdoor Large-Scale Cultivation of the Acidophilic Microalga Coccomyxa onubensis in a Vertical Close Photobioreactor for Lutein Production

et al. 2020

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“…, Fuentes et al. , Barcyt≐ and Nedbalová ). Strains of Chlorella are known to grow across a wide pH range (Mayo , Low‐Decarie et al.…”

Section: Extreme Environments and Classification Of Extremophile Algaementioning

confidence: 93%

“…, Fuentes et al. ). In the effluent from La Zarza‐Perrunal, a mine on the Iberian Pyrite Belt (IPB) algae such as Chlorella and some Zygnematales filaments were discovered (González‐Toril et al.…”

Section: Extremophile Microalgae In the Context Of Astrobiologymentioning

confidence: 99%

Extremophile Microalgae: the potential for biotechnological application

Malavasi

Soru

Cao

2020

Journal of Phycology

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Microalgae are photosynthetic microorganisms that use sunlight as an energy source, and convert water, carbon dioxide, and inorganic salts into algal biomass. The isolation and selection of microalgae, which allow one to obtain large amounts of biomass and valuable compounds, is a prerequisite for their successful industrial production. This work provides an overview of extremophile algae, where their ability to grow under harsh conditions and the corresponding accumulation of metabolites are addressed. Emphasis is placed on the high‐value products of some prominent algae. Moreover, the most recent applications of these microorganisms and their potential exploitation in the context of astrobiology are taken into account.

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Mixotrophic growth of a highly acidic habitat microalga for production of valuable fatty acids

Robles,

Ostojic,

Ruiz-Domínguez

et al. 2024

J Appl Phycol

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Photoautotrophic algal cultivation is challenging due to the shadowing effect produced by an increase in the number of cells; under such circumstances, mixotrophic growth might be an efficient alternative. We grew cultures of the autochthonous acidotolerant microalga Elliptochloris sp. on crude technical glycerin or glucose bubbled with either only air or air containing 2.5 % (v/v) CO2. We found that CO2 strongly influenced the production of Elliptochloris sp., as higher growth occurred in mixotrophy with CO2-enriched air compared to that with only air. Mixotrophy with CO2-enriched air allowed to reach higher biomass productivities and facilitated an increase in the relative abundance of saturated fatty acids. Nevertheless, mixotrophy with only air resulted in an increase of both saturated and unsaturated fatty acids, but biomass productivities were lower. These results, along with the limited biological contamination facilitated by low pH, suggest that this microalga might be attractive for large-scale production within the circular economy model.

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Phylogenetic characterization and morphological and physiological aspects of a novel acidotolerant and halotolerant microalga Coccomyxa onubensis sp. nov. (Chlorophyta, Trebouxiophyceae)

Cited by 20 publications

References 45 publications

Outdoor Large-Scale Cultivation of the Acidophilic Microalga Coccomyxa onubensis in a Vertical Close Photobioreactor for Lutein Production

Outdoor Large-Scale Cultivation of the Acidophilic Microalga Coccomyxa onubensis in a Vertical Close Photobioreactor for Lutein Production

Extremophile Microalgae: the potential for biotechnological application

Mixotrophic growth of a highly acidic habitat microalga for production of valuable fatty acids

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