Host factors do not influence the colonization or infection by fluconazole resistant Candida species in hospitalized patients

Yang, Yun-Liang; Cheng, Ming‐Fang; Chang, Ya‐Wen; Young, Ton-Ho; Chi, Hsin; Lee, Sai Cheong; Cheung, Bruno Man-Hon; Tseng, Fan-Chen; Chen, Tun‐Chieh; Ho, Yu-Huai; Shi, Zhi-Yuan; Chan, Chung-Huang Hubert; Lin, Jia Chun; Lo, Hsiu‐Jung

doi:10.1186/1477-5751-7-12

Cited by 10 publications

(7 citation statements)

References 20 publications

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“…On the other hand, some researches have found that the susceptibility degree of Candida species vary towards the used antifungal drugs (6, 7). For example, C. krusei and C. glabrata are resistant and less susceptible to fluconazole, respectively (7,8,12). Recent reports from different countries and hospitals have indicated that there has been an association between non-albicans and the rate of fluconazole resistance (12)(13)(14).…”

Section: Introductionmentioning

confidence: 99%

Antifungal susceptibility of Candida species isolated from candidura

Mahmoudabadi

Zarrin

Fard

2012

Jundishapur J Microbiol

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Candiduria is a common infection of the urinary tract. Evaluation of the susceptibility to antifungal drugs could be used for treatment and control of infection.Background: Candiduria is one of the most common symptoms of urinary tract infections caused by several species of Candida spp.. Several antifungals are available to treat such candidal infections. During the last decades, resistance to antifungal especially to non-albicans species has increased. Objectives: The present study aimed to evaluate the susceptibility to antifungal drugs of Candida species isolated from candiduria in Ahvaz. Materials and Methods: Ninety three species of yeasts and yeast like organisms isolated from urine samples [Candida albicans (58), C. glabrata (25), C. tropicalis (4), C. krusei (1), unknown Candida species (4) and Geotrichum species (1)] were used for susceptibility tests. All species were re-identified based on standard mycological methods. Then a suspension of each isolate of overnight cultures was prepared in 1ml of sterile PBS and adjusted to 0.5 McFarland turbidity standards. In the present study several antifungal drugs (fluconazole, amphotericine B, ketoconazole, econazole, itraconazole) were used for susceptibility test using disk diffusion method. Results:In the present study all tested isolates were sensitive/dose dependent to amphotericine B and nystatin, whereas only one isolate of C. glabrata was resistant to both antifungals. Resistance against fluconazole (48.4%) and ketoconazole (26.9%) were observed among tested isolates. Resistance against fluconazole was detected among all tested organisms, 34.4% of C. albicans, and 7.5% of C. glabrata. On the other hand, all isolates were sensitive to econazole (93.5% sensitive, 6.5% dose dependent). Conclusions: It was concluded that Candida species isolated from candiduria in hospitalized patients had excellent in vitro sensitivity against econazole. Other suitable antifungal drugs were amphotericine B and nystatin, itraconazole. Whereas, resistance against ketoconazole (26.9%) and especially fluconazole (48.4%) was significant.

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Section: Introductionmentioning

confidence: 99%

Antifungal susceptibility of Candida species isolated from candidura

Mahmoudabadi

Zarrin

Fard

2012

Jundishapur J Microbiol

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“…The supernatant was discarded and a drop of the deposit was examined microscopically at high magnification for pus cells. Pus cells ≥5 per high power field were considered to indicate infection (26). Emergent yeast colonies were stored for identification.…”

Section: Specimen Collection and Processingmentioning

confidence: 99%

“…Clean-catch mid-stream urine was collected into sterile universal container containing few crystals of boric acid as preservative (26).…”

Section: Specimen Collection and Processingmentioning

confidence: 99%

Candiduria among HIV- Infected Patients Attending a Tertiary Hospital in Benin City

Esebelahie¹,

Enweani²,

Newton-Esebelahie³

et al. 2014

Af J Clin Exp Micro

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Background: Candiduria is a common finding. However, in immunocompromised patients like HIV-infected individuals, it has high risk of morbidity and mortality as it could be a pointer to systemic candidiasis. Unfortunately, there are no clear criteria for differentiating between colonization and infection or between upper or lower urinary tract infections.Objective: This study focused on determining the spectrum of Candida species implicated in candiduria among HIV-infected individuals and their susceptibility to fluconazole and voriconazole in a tertiary hospital.Methods: A total of 300 subjects comprising of 200 HIV patients and 100 non-HIV individuals were used for this study. Clean catch midstream were collected from each individual and processed using standard microbiological techniques. Emergent Candida isolates were identified with CHROMagar Candida and sugar fermentation tests.Results: The overall prevalence of candiduria among HIV patients was 13.5%. HAART-naive patients had a significantly higher prevalence (OR=4.165, 95%CI=1.602, 10.828; P=0.0038) than their counterpart on highly active antiretroviral therapy (HAART). Female gender was a significant risk factor for acquiring candiduria. Age had no significant effect on the prevalence of candiduria in this study. A CD4+ count <200 cells/µl was a significant risk factor for acquiring candiduria only among HAART-naive patients (OR=11.711; 95%CI=3.943, 34.780; P= 0.0001). The three species of Candida recovered from this study were C. albicans, C. krusei and C.parapsilosis. C. albicans (64.52%, 83.36%) and C. krusei (66.67%, 100.00%) were resistant to fluconazole and voriconazole respectively.Conclusion: There is a significant relationship between antiretroviral therapy, CD4+ counts, and the prevalence of candiduria among the study population.Keywords:HAART, HAART-naive, candiduria, CD4+ counts, Candida, prevalence. CANDIDURIE CHEZ DES MALADES VIVANT AVEC LE VIH DANS UN HOPITAL TERTIAIRE DANS LA VILLE DE BENIN

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“…Predisposing factors include particularly prolonged and increased use of antifungal agents [1] and patients with compro mised immune systems, such as HIV-positive patients [2] and pregnant mothers with asymptomat ic vaginal candidiasis who run the risk of preterm delivery [3]. Amongst the species most frequently isolated are Candida albicans followed by Candida glabrata, Candida tropicalis and Candida krusei [4].…”

Section: Introductionmentioning

confidence: 99%

Strengths and Limitations of different Chromogenic Media for the Identification of <i>Candida</i> Species

Messeir¹,

Abrantes²,

Africa³

2012

MICROBIOLOGY

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The treat ment of invasive candidiasis and other Candida infections with the appropriate antifungal agent is assisted by the identification of Candida isolates to the species level. Rapid and accurate methods of differentiation are therefore imperat ive if t reat ment is to be effective, particularly in HIV-positive patients and in pregnant mothers where intervention may be necessary to reduce the risk for preterm delivery. The time used for isolation, identificat ion and detection of mixed cultures may be reduced with the help of available chro mogenic med ia. In this study, five commercial chro mogenic med ia were evaluated for the differentiation of Candida species. Six type-strains of Candida species were streaked onto each of five different chro mogenic media and incubated for up to 4 days at the different temperatures recommended by the manufacturers. This co mparative evaluation demonstrated the strengths and weaknesses of each mediu m employed and found CHROMagar™ Candida and Chro mogenic Candida Agar to be the most effective for distinguishing between different Candida species.

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Host factors do not influence the colonization or infection by fluconazole resistant Candida species in hospitalized patients

Cited by 10 publications

References 20 publications

Antifungal susceptibility of Candida species isolated from candidura

Antifungal susceptibility of Candida species isolated from candidura

Candiduria among HIV- Infected Patients Attending a Tertiary Hospital in Benin City

Strengths and Limitations of different Chromogenic Media for the Identification of <i>Candida</i> Species

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