Antifungal susceptibility of Candida species isolated from candidura

Abstract

Candiduria is a common infection of the urinary tract. Evaluation of the susceptibility to antifungal drugs could be used for treatment and control of infection.Background: Candiduria is one of the most common symptoms of urinary tract infections caused by several species of Candida spp.. Several antifungals are available to treat such candidal infections. During the last decades, resistance to antifungal especially to non-albicans species has increased. Objectives: The present study aimed to evaluate the susceptibility to antifungal drugs of Candida species isolated from candiduria in Ahvaz. Materials and Methods: Ninety three species of yeasts and yeast like organisms isolated from urine samples [Candida albicans (58), C. glabrata (25), C. tropicalis (4), C. krusei (1), unknown Candida species (4) and Geotrichum species (1)] were used for susceptibility tests. All species were re-identified based on standard mycological methods. Then a suspension of each isolate of overnight cultures was prepared in 1ml of sterile PBS and adjusted to 0.5 McFarland turbidity standards. In the present study several antifungal drugs (fluconazole, amphotericine B, ketoconazole, econazole, itraconazole) were used for susceptibility test using disk diffusion method. Results:In the present study all tested isolates were sensitive/dose dependent to amphotericine B and nystatin, whereas only one isolate of C. glabrata was resistant to both antifungals. Resistance against fluconazole (48.4%) and ketoconazole (26.9%) were observed among tested isolates. Resistance against fluconazole was detected among all tested organisms, 34.4% of C. albicans, and 7.5% of C. glabrata. On the other hand, all isolates were sensitive to econazole (93.5% sensitive, 6.5% dose dependent). Conclusions: It was concluded that Candida species isolated from candiduria in hospitalized patients had excellent in vitro sensitivity against econazole. Other suitable antifungal drugs were amphotericine B and nystatin, itraconazole. Whereas, resistance against ketoconazole (26.9%) and especially fluconazole (48.4%) was significant.

In Vitro Susceptibilities of Candida albicans Isolates to Antifungal Agents in Tokat, Turkey

“…In our study, 34% of the C. albicans isolates with MIC90 > 256 mg/L were found to be resistant to fluconazole. Similarly, Zarei Mahmoudabadi et al ( 33 ) showed that 55.2% of the C. albicans strains isolated from candiduria were resistant to fluconazole. In another study reported by the same authors, the resistance rate of C. albicans to fluconazole was 59.2% ( 34 ).…”

Chemical composition and antifungal activity of essential oil ofHyssopus officinalisL. from Bulgaria against clinical isolates ofCandidaspecies

“…In comparison with the MIC values of hyssop oils from various geographic regions, [12,26,31] the studied Bulgarian hyssop oil demonstrated stronger inhibitory activity against medically important representatives of C. albicans, C. tropicalis and C. krusei. This fact could be explained by the more complex chemical composition of the hyssop oil from Bulgaria.…”

Antifungal Susceptibility of Candida albicans Isolates at a Tertiary Care Hospital in Bulgaria

“…According to the Clinical and Laboratory Standards Institute (CLSI) and the European Committee on Antimicrobial Susceptibility Testing (EUCAST), the antifungal susceptibility of Candida spp. should be determined by broth microdilution method, yet disk-diffusion assay and agarbased E-test are also used (5,6,8,17,18). Recently, CHROMagar Candida was used for isolation and presumptive identification of the main yeast species, yet precise identification and susceptibility testing have to be performed in clinically important fungi.…”