Horse ooplasm supports in vitro preimplantation development of zebra ICSI and SCNT embryos without compromising YAP1 and SOX2 expression pattern

Gambini, A.; Rodriguez, M. D.; Rodríguez, María Belén; Briski, O.; Bragulat, Ana Paula Flores; Demergassi, Natalia; Losinno, Luis; Salamone, D.

doi:10.1371/journal.pone.0238948

Cited by 13 publications

(11 citation statements)

References 62 publications

(76 reference statements)

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“…After this evaluation, the sperm were injected into the oocytes of horses and were able to develop as far as the blastocyst stage. This result highlights that horses can be models for wild equines, as oocytes support hybrid development without compromising the expression and localization of cell differentiation markers and blastocyst cell number (Gambini et al, 2020).…”

Section: Heterologous In Vitro Fertilizationmentioning

confidence: 89%

“…Recently, a 6% rate of blastocyst formation was observed using the ICSI of Burchell's zebra sperm in horse oocytes (Gambini et al, 2020).…”

Section: In Vitro Fertilization and Embryo Culturementioning

confidence: 99%

“…This technique has been applied to assess the fertility of freeze-dried sperm of giraffe (Giraffa camelopardalis reticulata), using mouse oocytes, with 100% pronucleus formation observed (Kaneko et al, 2014). Gambini et al (2020) provided valuable information regarding the manipulation of zebra sperm and used porcine oocytes to estimate its ability to induce activation after ICSI, which showed 30% pronucleus formation. After this evaluation, the sperm were injected into the oocytes of horses and were able to develop as far as the blastocyst stage.…”

Section: Heterologous In Vitro Fertilizationmentioning

confidence: 99%

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Embryo production by in vitro fertilization in wild ungulates: progress and perspectives – A Review

Santos

Silva

Pereira

2022

Annals of Animal Science

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Wild ungulates are of fundamental importance for balancing ecosystems, as well as being the species of economic interest. Increasing concern over the accelerated population reduction of these species has resulted in the development of assisted reproduction techniques, such as in vitro fertilization (IVF), as a tool for conservation and multiplication. In the present scenario, IVF protocols were developed based on the methodologies used for domestic ungulates. Nevertheless, owing to the physiological and reproductive differences among the species, several factors associated with IVF and its relationship with the characteristics of the species of interest require clarification. In vitro conditions for the collection and selection of female and male gametes, oocyte maturation, sperm capacitation, co-incubation of gametes, and embryonic development can influence IVF results. Therefore, the present review considers the main advances in the methodologies already used for wild ungulates, emphasizing the strategies for improving the protocols to obtain better efficiency rates. Additionally, we discuss the conditions of each IVF stage, with emphasis on aspects related to in vitro manipulation and comparability with the protocols for domestic ungulates.

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Section: Heterologous In Vitro Fertilizationmentioning

confidence: 89%

“…Recently, a 6% rate of blastocyst formation was observed using the ICSI of Burchell's zebra sperm in horse oocytes (Gambini et al, 2020).…”

Section: In Vitro Fertilization and Embryo Culturementioning

confidence: 99%

Section: Heterologous In Vitro Fertilizationmentioning

confidence: 99%

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Embryo production by in vitro fertilization in wild ungulates: progress and perspectives – A Review

Santos

Silva

Pereira

2022

Annals of Animal Science

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“…Collectively, the results of the current investigation aimed to devise and optimize intracellular strain-related parameters that turned out to vary depending on different speeds of penetrating the porcine oocytes with the use of robotic (i.e., motorized) micro-manipulators might contribute to improvement of the efficiency of a variety of modern assisted reproductive technologies (ARTs). Taking the above-mentioned findings into consideration, the augmented efficiencies of microsurgical in vitro fertilization by ICSI and cloning by somatic cell nuclear transfer (SCNT) will be of especially great importance not only in pigs but also in other mammalian species [25][26][27][28][29][30][31].…”

Section: Discussionmentioning

confidence: 99%

Oocyte Penetration Speed Optimization Based on Intracellular Strain

et al. 2022

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Oocyte penetration is an essential step for many biological technologies, such as animal cloning, embryo microinjection, and intracytoplasmic sperm injection (ICSI). Although the success rate of robotic cell penetration is very high now, the development potential of oocytes after penetration has not been significantly improved compared with manual operation. In this paper, we optimized the oocyte penetration speed based on the intracellular strain. We firstly analyzed the intracellular strain at different penetration speeds and performed the penetration experiments on porcine oocytes. Secondly, we studied the cell development potential after penetration at different penetration speeds. The statistical results showed that the percentage of large intracellular strain decreased by 80% and the maximum and average intracellular strain decreased by 25–38% at the penetration speed of 50 μm/s compared to at 10 μm/s. Experiment results showed that the cleavage rates of the oocytes after penetration increased from 65.56% to 86.36%, as the penetration speed increased from 10 to 50 μm/s. Finally, we verified the gene expression of oocytes after penetration at different speeds. The experimental results showed that the totipotency and antiapoptotic genes of oocytes were significantly higher after penetration at the speed of 50 μm/s, which verified the effectiveness of the optimization method at the gene level.

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“…Our research group reported the production of the first zebra ( Equus quagga ) cloned embryos using oocytes from domestic horses with no differences in the expression of trophectoderm or inner cell mass proteins (Gambini, Duque Rodríguez, et al, 2020). Also, in 2020, the partnership of the Frozen Zoo at San Diego Zoo Wildlife Alliance, Revive and Restore, and ViaGen Pets and Equine reported, the birth of the first Przewalski's horse ( Equus ferus przewalskii ) (Revive and Restore, 2020), an endangered species that belongs to the same genus than horses, zebras, and donkeys.…”

Section: Reproductive Scntmentioning

confidence: 99%

State of the art of nuclear transfer technologies for assisting mammalian reproduction

Gambini

Briski

Canel

2022

Molecular Reproduction Devel

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The transfer of nuclear genomic DNA from a cell to a previously enucleated oocyte or zygote constitutes one of the main tools for studying epigenetic reprogramming, nucleus-cytoplasm compatibility, pluripotency state, and for genetic preservation or edition in animals. More than 50 years ago, the first experiences in nuclear transfer began to reveal that factors stored in the cytoplasm of oocytes could reprogram the nucleus of another cell and support the development of an embryo with new genetic information. Furthermore, when the nuclear donor cell is an oocyte, egg, or a zygote, the implementation of these technologies acquires clinical relevance for patients with repeated failures in ART associated with poor oocyte quality or mitochondrial dysfunctions. This review describes the current state, scope, and future perspectives of nuclear transfer techniques currently available for assisting mammal reproduction.

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Horse ooplasm supports in vitro preimplantation development of zebra ICSI and SCNT embryos without compromising YAP1 and SOX2 expression pattern

Abstract: UBACyT from the University of Buenos Aires. http://cyt.rec. uba.ar. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Cited by 13 publications

References 62 publications

Embryo production by in vitro fertilization in wild ungulates: progress and perspectives – A Review

Embryo production by in vitro fertilization in wild ungulates: progress and perspectives – A Review

Oocyte Penetration Speed Optimization Based on Intracellular Strain

State of the art of nuclear transfer technologies for assisting mammalian reproduction

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