Oocyte Penetration Speed Optimization Based on Intracellular Strain

Liu, Yaowei; Cui, Maosheng; Zhang, Yidi; Zhao, Xiangfei; Sun, Mingzhu; Zhao, Xin

doi:10.3390/mi13020309

Cited by 3 publications

(2 citation statements)

References 29 publications

(32 reference statements)

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“…Additionally, it has been reported that the speed at which the micropipette penetrates cells can have an impact on cell deformation and damage, and optimizing the penetration speed can reduce the damage caused by micropipette penetration to cells [ 25 ]. This inspires the potential improvement of the work in this paper.…”

Section: Discussionmentioning

confidence: 99%

Robotic Intracellular Pressure Measurement Using Micropipette Electrode

Qiu

et al. 2023

Sensors

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Intracellular pressure, a key physical parameter of the intracellular environment, has been found to regulate multiple cell physiological activities and impact cell micromanipulation results. The intracellular pressure may reveal the mechanism of these cells’ physiological activities or improve the micro-manipulation accuracy for cells. The involvement of specialized and expensive devices and the significant damage to cell viability that the current intracellular pressure measurement methods cause significantly limit their wide applications. This paper proposes a robotic intracellular pressure measurement method using a traditional micropipette electrode system setup. First, the measured resistance of the micropipette inside the culture medium is modeled to analyze its variation trend when the pressure inside the micropipette increases. Then, the concentration of KCl solution filled inside the micropipette electrode that is suitable for intracellular pressure measurement is determined according to the tested electrode resistance–pressure relationship; 1 mol/L KCl solution is our final choice. Further, the measurement resistance of the micropipette electrode inside the cell is modeled to measure the intracellular pressure through the difference in key pressure before and after the release of the intracellular pressure. Based on the above work, a robotic measurement procedure of the intracellular pressure is established based on a traditional micropipette electrode system. The experimental results on porcine oocytes demonstrate that the proposed method can operate on cells at an average speed of 20~40 cells/day with measurement efficiency comparable to the related work. The average repeated error of the relationship between the measured electrode resistance and the pressure inside the micropipette electrode is less than 5%, and no observable intracellular pressure leakage was found during the measurement process, both guaranteeing the measurement accuracy of intracellular pressure. The measured results of the porcine oocytes are in accordance with those reported in related work. Moreover, a 90% survival rate of operated oocytes was obtained after measurement, proving limited damage to cell viability. Our method does not rely on expensive instruments and is conducive to promotion in daily laboratories.

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Section: Discussionmentioning

confidence: 99%

Robotic Intracellular Pressure Measurement Using Micropipette Electrode

Qiu

et al. 2023

Sensors

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“…Although the success rate of robotic cell penetration is very high, the development potential of oocytes after penetration was not improved significantly compared with manual operations. The authors optimized the oocyte penetration speed based on the intracellular strain [ 11 ]. The authors present a microfluidic system for high-resolution nanoparticle separation based on negative magnetophoresis; this may serve as a versatile tool for separating nanometric objects of environmental or biological importance, such as nanoparticles, viruses, and other biological agents [ 12 ].…”

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confidence: 99%