Homogeneous multiplexed digital detection of microRNA with ligation-rolling circle amplification

Abstract

MicroRNA was transformed into a DNA nanoflower ball by LRCA reaction for homogeneous multiplexed digital detection using flow cytometry.

Dual-amplified CRISPR-Cas12a bioassay for HIV-related nucleic acids

“…A nucleic acid amplification strategy is often used to improve the detection limit of DNA or RNA analysis. 1 In addition to the classic polymerase chain reaction (PCR), many other nucleic acid amplification methods have been developed, such as rolling circle amplification (RCA), 2,3 hybridization chain reaction (HCR), 4 loop-mediated isothermal amplification (LAMP), 5 recombinase polymerase amplification (RPA), 6 etc. These strategies can effectively amplify the target nucleic acid by several orders of magnitude.…”

Accurate Detection of Target MicroRNA in Mixed Species of High Sequence Homology Using Target-Protection Rolling Circle Amplification

“… 1 , 6 , 24 Some previous studies have shown noticeable detection specificity; however, either only let 7a 26 or some different microRNAs having great nucleotide sequence variations were used as the targets. 22 When the duplex contains only one mismatch (such as miRNA family members), its hybridization behavior could be very close to that of the perfect-match duplex depending upon the chemical nature and position of the mismatch. Thus, it is relatively difficult to discriminate the target precisely from mixtures of high sequence homology.…”

A self-quenching fluorescence probe-mediated exponential isothermal amplification system for highly sensitive and specific detection of microRNAs

“…As shown in Table S2 (ESI †), the detection efficiency, detection range, LOD, and other performance metrics displayed in our approach were better than others. [18][19][20][21][22][23][24][25][26][27] Without a doubt, this ultrasensitive detection threshold can be ascribed to following reasons: (i) the adoption of the self-quenching fluorescence probes makes detective signal-tobackground ratio greatly improved. Thus, the targets at lower concentrations can be easily detected; (ii) only two kinds of probes are employed in the system, the occurrence of non-specific amplification is avoided, and the exponential growth of products and signals is also guaranteed.…”