A self-quenching fluorescence probe-mediated exponential isothermal amplification system for highly sensitive and specific detection of microRNAs

Abstract

A novel self-quenching fluorescence probe-mediated isothermal amplification system was developed, making highly sensitive and specific detection of miRNAs feasible.

Target-Induced Stepwise Disintegration of Starlike Branched and Multiplex Embedded Systems for Amplified Detection of Serum MicroRNA

“…Under ideal settings, researchers in DNA biosensors have been able to rationally design intelligent IAT-based methods to quantitatively detect different targets of interest and scale-up system functionality by combining them with unique proteinases that serve as powerful ″toolboxes″ in DNA nanotechnology. For example, with DNA polymerase and nickase, Zhao et al reported a cascade amplification system for miRNA detection via the cooperation of DNA polymerization and nicking . Our group has also developed a feedback system based on three hairpin pivots and rolling circle amplification (RCA) for the determination of miRNA .…”

“…For example, with DNA polymerase and nickase, Zhao et al reported a cascade amplification system for miRNA detection via the cooperation of DNA polymerization and nicking. 24 Our group has also developed a feedback system based on three hairpin pivots and rolling circle amplification (RCA) for the determination of miRNA. 9 However, these approaches still rely on the introduction of multicomponent systems consisting mostly of single-and double-stranded oligonucleotide probes, which can lead to high false-positive signals due to their susceptibility to nuclease degradation.…”

Determination of microRNA-122 in hepatocytes by two-step amplification of duplex-specific nuclease with laser-induced fluorescence detection

“…20,21 They are all ideal alternatives for miRNA assay. 22,23 Duplex-specic nuclease (DSN) is a special enzyme that can specically cleave double-stranded DNA or the DNA section of a DNA-RNA hybrid duplex, but it is inactive toward RNA or single-stranded DNA. [24][25][26] These characteristics endowed DSN with the ability to construct miRNA biosensors.…”