HISTOPATOLOGIA COMPARATIVA EM FÍGADOS DE BOVINOS, BUBALINOS E OVINOS INFECTADOS POR Fasciola hepatica

Abstract

Através técnicas de rotina para histopatologia foram examinados fígados de bovinos, bubalinos e ovinos infectados por Fasciola hepatica. As colorações utilizadas foram Hematoxilina- Eosina, Tricrômico de Schorr, Tricrômico de Mallory e Azul de Toluidina. As principais lesões observadas foram cortes de parasitas em ductos biliares, fibrose portal, hiperplasia de ducto biliar, fibrose de parede de ducto biliar e infiltração por linfócitos, plasmócitos, eosinófilos e neutrófilos. Lesões de ductos biliares mais calibrosos são observados mais acentuadamente em bovinos e bubalinos que em ovinos. No entanto, a eosinofilia está presente de forma mais acentuada em ovinos que nas demais espécies. Comparando os resultados obtidos, conclui-se que a fasciolose em bovinos e bubalinos se trata de uma doença crônica. Nos ovinos o predomínio de eosinófilos caracteriza fase inicial de fasciolose crônica. Hepatopathology of infection by Fasciola hepatica in cattle, buffaloes and sheep Abstract A histopathological study on hepatic lesions caused by Fasciola hepatica has been carried out three domestic ruminants: cattle, buffalo’s and sheep. Liver samples were processed by routine histological methods. The staining procedures included hematoxilin-eosine, Schorr (triple staining), Mallory (triple staining) and toluidine blue. The most frequent lesions found were the following ones: parasite sections inside the billiary ducts, portal system fibrosis and billiary duct hyperplasia. Lymphocytes, plasmocytes, eosinophils and neutrophils, characteristics of a chronic disease, were found in bovines and bubalines. The presence of eosinophils in sheeps was characteristic of the initial phase of a chronic fascioliasis.

HISTOPATHOLOGY ASPECTS AND FIBROSIS EVALUATION OF BOVINE NATURALLY INFECTED LIVERS BY Fasciola hepatica

“…Regarding the eosinophilic infiltration, Bostelmann et al (2000) also found discrete infiltration of eosinophils in bovine livers with Fasciola sp. and related moderate eosinophilic infiltration in theirs samples.…”

“…Kishida et al (2013) found papillary and glandular hyperplasia of the biliary epithelium in liver obtained from meat inspection in Japan and found moderate biliary epithelial hyperplasia in 33,33% samples following by 66,66% severe. However, Bostelmann et al (2000) evaluated bovine liver samples condemned of fasciolosis from a slaughterhouse in Parana State, Brazil, and found a moderate hyperplasia of the biliary ducts in all the evaluated samples. in their studies also found a moderate intensity of ductal hyplerplasia.…”

“…Bostelmann et al (2000) evaluated the inflammatory infiltration in bovine livers infected by Fasciola hepatica and found lymphocytes, plasmocytes, eosinophils, and mastocytes distributed in the medial third of the most calibre ductal wall, besides lymphoplasmacytic and neutrophilic infiltration in the outer third of ductal wall. The lymphoplasmacytic infiltration was moderate.…”

“…Macroscopically, the injuries generate discard of the liver and considerable economic losses. Microscopically, the damage is considerable and undermine integrity of liver function, and commonly include injuries in biliary ducts, calcification, fibrosis and inflammatory infiltration (Bostelmann et al, 2000;Kishida et al, 2013). To evaluate an affected bovine liver and to establish a histopathological classification of injury would facilitate the understanding of pathogenesis and the level of involvement (Riet-Correa et al 2001).…”

Achados de formas jovens e adultas de Fasciola hepatica (Linnaeus, 1758) em bovinos durante inspeção post-mortem

“…A doença é causada por Fasciola hepatica, parasito de grandes e pequenos ruminantes e várias espécies de animais silvestres e domésticos (SILVA SANTOS; SCAINI; RODRIGUES, 1992;MARQUES;SCROFERNEKER, 2003;ARAÚJO et al, 2007;AMOR et al, 2011). A ação do helminto causa prejuízo econômico à pecuária mundial devido à condenação de fígados dos animais afetados (BOSTELMANN et al, 2000;MWABONIMANA et al, 2009;BALA;GARBA;YAZAH, et al, 2011;BERNARDO et al, 2011;OZUNG;OWAI;ONI, 2011). Paralelamente é registrada queda na produção, atraso no crescimento e mortalidade.…”