Histopathological study of Schistosoma mansoni infection in the murine model using the PC (Pará) and LILA (Maranhão) strains

Abstract

Experimental models of Schistosoma mansoni infections in mammals have contributed greatly in understanding the pathology and pathogenesis of human infection. The absence of earlier reviews regarding specific strains of the Amazon region prompted research, which the main objective was to describe histopathological lesions in different phases of schistosomiasis in a murine model using PC (Pará) and LILA (Maranhão) S. mansoni strains. One hundred and eighty young female albino swiss mice (Mus musculus) were used and were randomly divided into five groups and controls), according to the number of cercariae injected and the strain adopted. Animals were sacrificed in predetermined periods (35, 56, 112, 156, and 180 days) in an attempt to follow the evolution of the disease in the histological sections of their tissues at different phases of infection. Our findings were compatible with the data already described by others authors using different strains of S. mansoni, making it possible to identify some peculiarities, which are discussed in this work. In conclusion, the strains of parasite used did not modify the histopathological findings in the tissues of infected mice in any significant way when compared with the results of other studies using different strains.

Parasitological and histological aspects of Holochilus sciureus naturally infected by Schistosoma mansoni

“…Macro and microscopic analysis revealed changes on the liver surface (whitish areas, fibrosis, and granulomas) similar to the results of experimental studies with Mus musculus (CARVALHO et al, 1986;AMARAL et al, 2017) and Mus spretus (PÉREZ DEL VILLAR et al, 2013). The liver is the most affected organ by the infection and presence of of S. mansoni eggs (SILVA-SOUZA & VASCONCELOS, 2005;LOPES et al, 2006;SILVA et al, 2012;PÉREZ DEL VILLAR et al, 2013).…”

Could diet composition modulate pathological outcomes in schistosomiasis mansoni? A systematic review of in vivo preclinical evidence

“…Human infections often occur from heterogeneous parasites, inoculum and time of evolution; aspects linked to variable profiles of morbidity and mortality (Abdul-Ghani and Hassan, 2010). Since these aspects are difficult to control in human contexts, preclinical models remain highly relevant to improve understanding of host–pathogen interaction, as well as the impact of environmental factors on schistosomiasis evolution (Lopes et al 2006).…”

Human study and experimental behavior of Schistosoma mansoni isolates from patients with different clinical forms of schistosomiasis

“…The authors concluded that the results in mice probably cannot be used to predict possible differences in behavior of geographical strains of S. mansoni in infected person. Other histopathological study of S. mansoni infection in the murine model using the PC (Pará) and LILA (Maranhão) strains also did not showed significant differences between the strains (Lopes et al, 2006). No parasitological and histopathological differences between geographical strains of S. mansoni from Puerto Rico, Brazil (Belo Horizonte), Saint Lucia and Tanzania (Mwanza) were noted by Powers and Cheever (1972) in a study in mice and Rhesus monkeys using different inocula, confirming the previous results.…”