Histopathological effects in gills and liver of Sparus aurata following acute and chronic exposures to erythromycin and oxytetracycline

Rodrigues, Sara; Antunes, Sara C.; Nunes, Bruno; Correia, Alberto Teodorico

doi:10.1007/s11356-019-04954-0

Cited by 51 publications

(36 citation statements)

References 78 publications

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“…After chronic exposure, inflammatory changes were observed. Rodrigues et al [48] demonstrated tissue alterations in the gills and liver of Sparus aurata individuals acutely (96 h) and chronically (28 days) exposed to oxytetracycline (0.0004-400 µg/L). Some circulatory, regressive, progressive, and inflammatory alterations were noted in both tested fish organs in all exposed individuals.…”

Section: Discussionmentioning

confidence: 99%

Effects of Oxytetracycline and Gentamicin Therapeutic Doses on Hematological, Biochemical and Hematopoietic Parameters in Cyprinus carpio Juveniles

Kondera

Bojarski

Ługowska

et al. 2020

Animals

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Hematological, biochemical and hematopoietic effects of therapeutic doses of two antibiotics, oxytetracycline (OTC) and gentamicin (GEN), in clinically healthy common carp juveniles were studied. The fish were divided into four groups: controls 1 and 2 (untreated or injected with 0.6% NaCl solution), and two groups treated with antibiotics (orally with 75 mg/kg OTC four times every two days or injected with a single dose (4 mg/kg) of GEN dissolved in 0.6% NaCl). Blood and head kidneys were sampled from all fish 3 days post-treatments for hematological, biochemical and hematopoietic tissue analyses. No major alterations in the values of hematological and serum biochemical parameters occurred following administration of OTC or GEN. Glucose concentrations were significantly lower in both groups of fish subjected to injections (Control 2 and GEN), while the oxidative metabolic activity of phagocytes increased in the antibiotic-treated groups (significantly in OTC). More alterations were observed in hematopoietic tissue. Immunocytochemical analysis revealed that G caused a significant increase in the rate of cell proliferation (PCNA-positive cells) and an increase in the frequency of apoptotic cells (caspase-positive). The frequency of lymphoid lineage decreased, which was related to a decrease in the abundance of mature lymphocytes in GEN-treated fish. Percentages of neutrophilic lineage were significantly elevated in OTC and GEN groups compared to controls. The obtained results showed no considerable hematotoxicity or hepatotoxicity of therapeutic doses of OTC and GEN to carp.

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Section: Discussionmentioning

confidence: 99%

Effects of Oxytetracycline and Gentamicin Therapeutic Doses on Hematological, Biochemical and Hematopoietic Parameters in Cyprinus carpio Juveniles

Kondera

Bojarski

Ługowska

et al. 2020

Animals

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“…The vacuolated cell frequency is increased in liver cells of treated fish as compared to the control. Nunes et al and Rodrigues et al [31,32] also observed vacuolization in cells of fish exposed to tetracyclines. Ahmad et al [30] used pentachlorophenol (0.1-0.4 ppm) in Heteropneustes fossilis and reported the induction of MNC in treated groups.…”

Section: Discussionmentioning

confidence: 91%

“…Furthermore, antibiotics inhibit DNA synthesis, which may be due to the disarrangement of molecular organization leading to degeneration [40]. NAs caused by OTC may be due to increased oxidative stress results in to break down of microtubules and F actin cytoskeleton [32]. The enlarged liver cells were also observed with or without concomitant increase in nuclear size.…”

Section: Discussionmentioning

confidence: 99%

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Cytotoxic and Genotoxic Effect of Oxytetracycline on Fish Cyprinus Carpio After Acute Exposure

Sharma¹,

Thakur

Verma

et al. 2019

Asian J Pharm Clin Res

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Objective: Cytotoxicity in blood cells and genotoxic alteration in gill, liver, and kidney of Cyprinus carpio acutely (96 h) exposed to 80 mg/L oxytetracycline (OTC) including a control (non-exposed group) were evaluated in the present study. Genotoxic endpoints reflecting different types of genetic damage in cells of the liver, gill, and kidney were also determined by analysis of nuclear and cytoplasmic abnormalities. Methods: C. carpio was divided into two groups, one control and others treated with 80 mg/L OTC. After 96 h, sampling was done and slides were prepared for different tissues as well as for measuring polychromatic erythrocyte (PCE) frequency in blood. Slides were scored for micronucleus, nuclear abnormalities, swollen cells, and vacuolated cytoplasm. Results: The hereby data obtained showed a higher and significant increase in the genotoxic effect in all the tissues tested. Furthermore, gill cells showed the highest genotoxic effect followed by liver and kidney, while PCE frequency increases up to 72 h of exposure, on the other hand, a significant decrease in the value was observed at 96 h of exposure. Conclusion: The present study revealed that OTC has cytotoxic and genotoxic effect on different organs and blood cells of C. carpio at this concentration and suggests gill as sensitive tissue for genotoxic assessment.

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“…It is well known that aquatic fish confront some exposures changes of the surrounding environment [1–4]. Salinity and alkalinity are closely associated with the toxicant outcome of aquatic animals [5, 6].…”

Section: Introductionmentioning

confidence: 99%

High‐throughput metabolomics method based on liquid chromatography‐mass spectrometry: Insights into the underlying mechanisms of salinity–alkalinity exposure‐induced metabolites changes in Barbus capito

Sun

Han

Yao

et al. 2020

J of Separation Science

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It is critical to investigate the adaptive development and the physiological mechanism of fish in external stimulation. In this study, the response of Barbus capito to salinity–alkalinity exposure was explored by high‐throughput nontargeted and liquid chromatography–mass spectrometry‐based metabolomics to investigate metabolic biomarker and pathway changes. Meanwhile, the biochemical indexes of Barbus capito were measured to discover the chronic impairment response to salinity–alkalinity exposures. A total of 29 tissue metabolites were determined to deciphering the endogenous metabolic changes of fishes during the different concentration salinity–alkalinity exposures environment, which were mainly involved in the key metabolism including the phenylalanine, tyrosine, and tryptophan biosynthesis, arachidonic acid metabolism, pyruvate metabolism, citrate cycle, and glycerophospholipid metabolism. Finally, we found the amino acid metabolism as key target was associated with the endogenous metabolites and metabolic pathways of Barbus capito to salinity–alkalinity exposures. In conclusion, metabolomics is a potentially powerful tool to reveal the mechanism information of fish in various exposure environments.

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Histopathological effects in gills and liver of Sparus aurata following acute and chronic exposures to erythromycin and oxytetracycline

Cited by 51 publications

References 78 publications

Effects of Oxytetracycline and Gentamicin Therapeutic Doses on Hematological, Biochemical and Hematopoietic Parameters in Cyprinus carpio Juveniles

Effects of Oxytetracycline and Gentamicin Therapeutic Doses on Hematological, Biochemical and Hematopoietic Parameters in Cyprinus carpio Juveniles

Cytotoxic and Genotoxic Effect of Oxytetracycline on Fish Cyprinus Carpio After Acute Exposure

High‐throughput metabolomics method based on liquid chromatography‐mass spectrometry: Insights into the underlying mechanisms of salinity–alkalinity exposure‐induced metabolites changes in Barbus capito

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