Cytotoxic and Genotoxic Effect of Oxytetracycline on Fish Cyprinus Carpio After Acute Exposure

Sharma, Mandeep; Thakur, J C; Verma, Subhash C.; Sharma, Pardeep

doi:10.22159/ajpcr.2019.v12i12.35740

Cited by 5 publications

(1 citation statement)

References 32 publications

(38 reference statements)

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“…Piscine poikilocytosis is induced by foreign particles, heavy metal toxicity, pharmaceuticals, and antibiotics, leading to altered erythrocyte morphology and anemic conditions [3,5,[13][14][15][16][17]. Dietary florfenicol and emamectin benzoate cause erythro-morphological alterations in healthy Oreochromis niloticus juveniles [2,3,11,12].…”

Section: Introductionmentioning

confidence: 99%

Visualization of poikilocytosis as an emerging erythrocytic biomarker for fish health assessment

Bardhan,

Abraham,

Das

et al. 2024

Animal Research and One Health

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Fish health assessment is essential for maintaining sustainable aquatic ecosystems and ensuring the well‐being of wild and farmed fish populations. Hematological parameters are crucial indicators of fish health, with poikilocytosis emerging as a fundamental marker with significant diagnostic value. Poikilocytosis refers to abnormally shaped erythrocytes in bloodstream, reflecting underlying physiological and pathological conditions. Poikilocytosis can occur in various fish species and can be influenced by environmental stressors, infectious agents, nutritional deficiencies, and exposure to pollutants. Morphological alterations in erythrocytes, such as acanthocytes, echinocytes, dacrocytes, schistocytes, spherocytes, and codocytes are common poikilocytes in fish. Understanding the relationship between poikilocytosis and fish health has important implications for disease diagnosis, monitoring, surveillance, and management. By quantifying poikilocytic changes, researchers and veterinarians can differentiate normal variations from pathological conditions, facilitating targeted interventions and treatment strategies. While most studies have focused on heavy metal toxicity, stressors, nutritional deficiencies, pollutants, and therapeutics, the etiological induction of poikilocytosis in fish health has been overlooked. Nonetheless, poikilocytosis remains a valuable biomarker for assessing fish health and their environment. This review highlights piscine poikilocytosis as a significant fish hematological biomarker and its importance in understanding their health and culture conditions.

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Section: Introductionmentioning

confidence: 99%

Visualization of poikilocytosis as an emerging erythrocytic biomarker for fish health assessment

Bardhan,

Abraham,

Das

et al. 2024

Animal Research and One Health

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Biological and histological changes and DNA damage in Oreochromis niloticus exposed to oxytetracycline: a potential amelioratory role of ascorbic acid

Abu-Zahra,

Atia,

Elseify

et al. 2023

Aquacult Int

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Antibiotics are crucial in the control of bacterial infections on fish farms. However, the influence of licensed antibiotics on DNA damage, histological changes, and protein differentiation in some farmed fish species is unidentified. The current study aimed to investigate the effect of therapeutic (80 mg/kg body weight/day) and high doses (160 mg/kg body weight/day) of oxytetracycline (OTC) on some biological indices, protein differentiation, histological changes, and DNA damage in Oreochromis niloticus (n = 360; 40 ± 3.5 g) and the potential positive effects of dietary ascorbic acid (AA) and whether it is capable of reducing the deleterious impacts of OTC and working synergistically to increase fish disease resistance and decrease morbidity and mortality rates. In the current study, fish were fed diets with or without OTC (80 or 160 mg/kg body weight/day) and AA (100 mg/kg diet/day) for 28 days, followed by a bacterial stress test with two different virulent strains for 14 days. Treatments include CTR (control; without additives), AA, OTC80, OTC160, OTC80 + AA, and OTC160 + AA. The results revealed that the AA group had the best antioxidative (significant increase in SOD, CAT, and GPx) and immunomodulatory effects (an increase in IgM, nitroblue tetrazolium, and total leucocytic count). Furthermore, the hematological parameters, immune indices (NBT), and antioxidant enzymes were significantly reduced by OTC160 (p ≤ 0.05). The results also showed that OTC160 notably augmented the activity of liver (ALT, AST, and LDH) and kidney (creatinine and urea) damage markers (p ≤ 0.05). OTC160 revealed considerable hepatic and renal tissue damage and significantly higher hepatocyte DNA damage. Pathological changes and DNA damage were directly correlated to the dosage of OTC exposure, where they were more severe in OTC160. OTC80 showed insignificant changes in most parameters. Compared to CTR, the inclusion of AA in the O. niloticus diet significantly restored the biological indices, histological changes, and DNA damage in OTC-treated groups. The morbidity and mortality percentages after bacterial challenge were significantly lower in the OTC80 + AA group, followed by the OTC160 + AA group than in the CTR. These results corroborate the use of AA as a potent antioxidant and immune stimulant and a potential co-adjuvant to OTC in the diet of O. niloticus to reach optimal resistance to disease.

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Hepatic and blood alterations in Lithobates catesbeianus tadpoles exposed to sulfamethoxazole and oxytetracycline

Rutkoski

Grott²,

Israel³

et al. 2022

Chemosphere

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Cytotoxic and Genotoxic Effect of Oxytetracycline on Fish Cyprinus Carpio After Acute Exposure

Cited by 5 publications

References 32 publications

Visualization of poikilocytosis as an emerging erythrocytic biomarker for fish health assessment

Visualization of poikilocytosis as an emerging erythrocytic biomarker for fish health assessment

Biological and histological changes and DNA damage in Oreochromis niloticus exposed to oxytetracycline: a potential amelioratory role of ascorbic acid

Hepatic and blood alterations in Lithobates catesbeianus tadpoles exposed to sulfamethoxazole and oxytetracycline

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