Histopathological characterization and fluorescence in situ hybridization of Cyprinid herpesvirus 2 in cultured Prussian carp, Carassius auratus gibelio in China

Ding, Zhengfeng; Xia, Siyao; Zhao, Zi-Ming; Xia, Aijun; Shen, Meifang; Tang, Jianguo; Xue, Hui; Geng, Xuebing; Yuan, Sheng

doi:10.1016/j.jviromet.2014.05.011

Cited by 21 publications

(19 citation statements)

References 29 publications

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“…Histological examination revealed areas of tissue necrosis in the liver, spleen and kidney of the examined fish, containing hypertrophic nuclei and showing pale staining inclusions and marginated chromatin. The presence of cells with these distinctive nuclear changes is consistent with observations in reports of CyHV-2 in goldfish (Jeffery et al 2007) and Prussian carp (Ding et al 2014).…”

supporting

confidence: 91%

“…In the first crucian carp analysed, the histological findings were in accordance with the histopathological lesion described in goldfish (Jeffery et al 2007) and Prussian carp (Ding et al 2014) CyHV-2 mortality events. Additionally, electron microscopy and molecular methods confirmed the presence of CyHV-2.…”

supporting

confidence: 79%

“…) and Prussian carp (Ding et al . ) CyHV‐2 mortality events. Additionally, electron microscopy and molecular methods confirmed the presence of CyHV‐2.…”

Section: Virological Examination Of Eight Crucian Carp and Five Commomentioning

confidence: 99%

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New detection of Cyprinid herpesvirus 2 in mass mortality event of Carassius carassius (L.), in Italy

Fichi

Susini

Cocumelli

et al. 2016

Journal of Fish Diseases

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supporting

confidence: 91%

supporting

confidence: 79%

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New detection of Cyprinid herpesvirus 2 in mass mortality event of Carassius carassius (L.), in Italy

Fichi

Susini

Cocumelli

et al. 2016

Journal of Fish Diseases

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“…(Jung & Miyazaki ; Ding et al . ; Lovy & Friend ). However, the intestinal lesions do not appear to always be present, for instance in C. gibelio (Wu et al .…”

Section: Discussionmentioning

confidence: 99%

First detection of Cyprinid Herpesvirus 2 (CyHV‐2) in goldfish (Carassius auratus) in France

Boitard¹,

Baud²,

Labrut

et al. 2015

Journal of Fish Diseases

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Massive mortalities of Carassius auratus (L.) occurred in a farm in France during summer 2014. Fish presented anorexia, loss of scales and large amounts of mucus on the gills. Necrosis of the distal tip of the filament and the lamellae, combined with fusion of the lamellae, was observed, as well as necrosis in the hematopoietic organs and in the digestive tract. The histological examination led to hypothesize the implication of a virus in the mortality. The presence of cyprinid herpesvirus 2 (CyHV-2) in dead fish was demonstrated by amplification and sequencing of portions of the DNA polymerase and helicase genes, both sequences exhibiting 100% identity with CyHV-2 from Japan. In an attempt to find genetic markers of variation, two regions containing tandem repeats in the Japanese genome were amplified from a virus-positive sample from the present outbreak. A first region (mB) was fully identical to the Japanese isolate. However, the second region (mA) exhibited a range of deletions and substitutions compared to CyHV-2 from Japan. This is the first report of CyHV-2 in France in association with mortality of goldfish and the first identification of a molecular marker for its tracing.

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“…Cyprinid herpesvirus 2 (CyHV‐2), initially known as goldfish herpesviral haematopoietic necrosis virus or herpesvirus haematopoietic necrosis virus of goldfish, is a member of Cyprinivirus in the family Alloherpesviridae (Davison et al, ) that include carp pox virus (CyHV‐1) and koi carp herpesvirus (CyHV‐3) (Hanson, Dishon, & Kotler, ). Since the first described on the epizootic of CyHV‐2 infection in Japan (Jung & Miyazaki, ), many were emerged around the world including Australia (Stephens, Raidal, & Jones, ), USA (Goodwin et al, ), UK (Philbey, ), France (Boitard et al, ), the Netherlands (Ito, Kurita, & Haenen, ) as well as China (Chang, Lee, Chiang, & Jong, ; Ding et al, ; Li et al, ; Wang et al, ) over the past 20 years. This virus was first thought to have virulence for only goldfish (Wang et al, ), but in recent years mass mortalities that caused by CyHV‐2 natural infection were occurred in gibel carp (Xu et al, ) and prussian carp (Danek et al, ; Ding et al, ; Fichi et al, ).…”

Section: Introductionmentioning

confidence: 99%

New detection of Cyprinid herpesvirus 2 associated with mass mortality in colour crucian carp ( Carassius auratus ), in China

Zhao

Geng

et al. 2019

Aquac Res

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During October 2016, a mass mortality of colour crucian carp (Carassius auratus), which the affected fish were lethargic, inappetence and anoxic, was occurred in a fish farm located in Chengdu, Sichuan province, China. To elucidate the aetiology of this outbreak, histological and electron microscope examination, molecular investigation were conducted. Pathologic examination revealed multi foci necrosis on haematopoietic organs, gills, hearts and pancreas. Transmission electron microscopy observations exhibited sphere herpesvirus‐like particles distributed amongst the tissues of gill, spleen and kidney. Molecular analysis is verified that the causative agent of this outbreak was Cyprinid herpesvirus 2 (CyHV‐2). This report first report CyHV‐2 in colour crucian carp, which increases the concern about damage of CyHV‐2 and its potential role in species.

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Histopathological characterization and fluorescence in situ hybridization of Cyprinid herpesvirus 2 in cultured Prussian carp, Carassius auratus gibelio in China

Cited by 21 publications

References 29 publications

New detection of Cyprinid herpesvirus 2 in mass mortality event of Carassius carassius (L.), in Italy

New detection of Cyprinid herpesvirus 2 in mass mortality event of Carassius carassius (L.), in Italy

First detection of Cyprinid Herpesvirus 2 (CyHV‐2) in goldfish (Carassius auratus) in France

New detection of Cyprinid herpesvirus 2 associated with mass mortality in colour crucian carp ( Carassius auratus ), in China

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