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In this paper, the sedimentation of a sphere and its radial migration in a Poiseuille flow in a vertical tube filled with a Newtonian fluid are simulated with a finite-difference-based distributed Lagrange multiplier (DLM) method. The flow features, the settling velocities, the trajectories and the angular velocities of the spheres sedimenting in a tube at different Reynolds numbers are presented. The results show that at relatively low Reynolds numbers, the sphere approaches the tube axis monotonically, whereas in a high-Reynolds-number regime where shedding of vortices takes place, the sphere takes up a spiral trajectory that is closer to the tube wall than the tube axis. The rotation motion and the lateral motion of the sphere are highly correlated through the Magnus effect, which is verified to be an important (but not the only) driving force for the lateral migration of the sphere at relatively high Reynolds numbers. The standard vortex structures in the wake of a sphere, for Reynolds number higher than 400, are composed of a loop mainly located in a plane perpendicular to the streamwise direction and two streamwise vortex pairs. When moving downstream, the legs of the hairpin vortex retract and at the same time a streamwise vortex pair with rotation opposite to that of the legs forms between the loops. For Reynolds number around 400, the wake structures shed during the impact of the sphere on the wall typically form into streamwise vortex structures or else into hairpin vortices when the sphere spirals down. The radial, angular and axial velocities of both neutrally buoyant and non-neutrally buoyant spheres in a circular Poiseuille flow are reported. The results are in remarkably good agreement with the available experimental data. It is shown that suppresion of the sphere rotation produces significant large additional lift forces pointing towards the tube axis on the spheres in the neutrally buoyant and more-dense-downflow cases, whereas it has a negligible effect on the migration of the more dense sphere in upflow.

The role of the gut microbiome has been a hot topic in recent years. One aim of this review is to shed light on the crosstalk between sex hormones and the gut microbiome. Researchers have observed a sex bias of the composition of the gut microbiome in mice and have proved that sex differences influence the composition of the gut microbiome, although the influence is usually obscured by genetic variations. Via cell studies, animal studies and some observational studies in humans, researchers have confirmed that the gut microbiome can be shaped by the hormonal environment. On other hand, some theories suggest that the gut microbiota regulates the levels of sex hormones via interactions among its metabolites, the immune system, chronic inflammation and some nerve-endocrine axes, such as the gut-brain axis. In addition, bidirectional interactions between the microbiome and the hormonal system have also been observed, and the mechanisms of these interactions are being explored. We further describe the role of the gut microbiome in sex hormone-related diseases, such as ovarian cancer, postmenopausal osteoporosis (PMOP), polycystic ovary syndrome and type 1 diabetes. Among these diseases, PMOP is described in detail. Finally, we discuss the treatments of these diseases and the application prospects of microbial intervention.

Streptococcus iniae has emerged as an important fish pathogen over the past few decades causing high losses in aquaculture farms all over the world. At least 27 species of fish have been documented to be infected by S. iniae, including cultured and wild populations. In August and October 2013, a serious infectious disease characterized by body ulcer, internal organs haemorrhages and nodules showing on epicardium occurred on the Acipenser baerii farms in Ya'an country, China. Histological examination revealed a multisystemic, necrotising inflammatory response that was particularly marked in liver, kidney, heart and brain. Mass mortality (>40%) was observed in infected fish and two Gram-positive cocci (Ab130920 and Ab131025) were obtained from kidneys and livers of diseased fish. Experimental infections with these two isolates resulted in marked symptoms in the sturgeons similar to those observed in natural outbreaks, and the LD 50 values of the two isolates were 5.1 9 10 5 and 6.4 9 10 5 cfu per fish respectively. The two microorganisms were identified as S. iniae through physiological and biochemical tests, 16S rRNA and lctO gene sequence analysis. Both two isolates showed a similar antibiotic susceptibility, which were sensitive to ampicillin, amoxicillin, cefazolin, amikacin, deoxycycline, florfenicol, azithromycin, ciprofloxacin, vancomycin and resistant to streptomycin, gentamicin, kanamycin, norfloxacin and sinomin (SMZ/TMP). Multiplex PCR assay for virulence genes showed both isolates possessed six main virulence genes: simA, scpI, pdi, pgm, cpsD and sagA genes. These results indicated that S. iniae could act as a pathogen of farmed A. baerii. This is the first report of S. iniae infection associated with mass mortality in A. baerii.

The effects of large neutrally buoyant particles on the flow instability and turbulence transition in pipe flow are investigated with the fictitious domain method. The periodic boundary condition is introduced in the streamwise direction. The work comprises two parts. In the first part, the pressure gradient is kept constant, and the purpose is to study the particle-induced flow instability. In our previous study [X. Shao, Z. Yu, and B. Sun, Phys. Fluids 20, 103307 (2008)10.1063/1.3005427], it was observed that a particle of a/R = 0.1 (a and R being the radii of the particle and the tube, respectively) induced the flow structure characterized by two pairs of weak and stable streamwise vortices at the Reynolds number of 1000. In the present study, our results show that the flow structure loses stability at the Reynolds number of 1500. However, it is interesting that the system eventually reaches a stable state: the particle spirals forward along the tube wall, accompanied by a stable flow structure for the case of one single particle in the computational domain. In the second part of the present study, the flow flux is kept constant, and the purpose is to examine the effects of particles on the critical Reynolds number based on the mean velocity. Our results show that large particles trigger the turbulence transition at low particle volume fractions, but delay the transition as the particle volume fraction exceeds a critical value, in agreement with the previous experimental observation [J.-P. Matas, J. F. Morris, and É. Guazzelli, Phys. Rev. Lett. 90, 014501 (2003)10.1103/PhysRevLett.90.014501].