Histopathological Changes of the Flaxseed Extract on Skin Wound Healing in Diabetic Rabbits

Abstract

BACKGROUND: Wound healing includes phases such as cell migration, extracellular matrix deposition, remodeling, and angiogenesis. There are growing medicines that accelerate wound healing, significantly herbal medications that mainly safe and reliable.
 AIM: The aim of the study is to examine the histological changes induced by flaxseed oil during wound healing in diabetic animal model.
 METHODS: Forty-five male white New Zealand rabbits divided into two main groups diabetic and non-diabetic each group is divided into three groups (n = 9).Diabetic animal group include: (1) Study group (adding Flaxseed), (2) positive control group (adding fucidin 2% cream), and (3) negative control group (no treatment) same distribution of non-diabetic animals groups. Four linearshape,full-thickness wounds were made in both sides of the backbone skin in each animal. Tissue samples were obtained at days 4th, 7th, and 14th post wounding for microscopical analysis, histopathological parameters included inflammation, re-epithelialization, neovascularization, and surface closure rates.
 RESULTS: The study groups (using flax seed) in both diabetic and non-diabetic groups show significant reduction in inflammatory cells infiltration at day 14 (0.67 ± 0.753) (1.67 ± 0.516), and noticeable increase in wound closure rate (4.00+-0.000). Both diabetic and non-diabetic animal groups show high re-epithelialization potential at day 4 and day 7, respectively (2.00 ± 1.549; 4.00 ± 0.00). Diabetic animal study group using flaxseed shows high neovascularization rate at the day 14 (2.17 ± 0.753) compared with positive control diabetic animal group (2.00 ± 0.000).
 CONCLUSION: Topical application of flaxseed promoted healing process particularly in diabetic animal model by shortening inflammation phase, elevating surface closure rate, promoting re-epithelialization process, and enhancing neovascularization, flaxseed is suggested as a potential effective herbal base medication for facilitating wound healing.

Evaluation of Biomedical Applications for Linseed Extract: Antimicrobial, Antioxidant, Anti-Diabetic, and Anti-Inflammatory Activities In Vitro

“…In vivo, the anti-inflammatory and analgesic potentials of linseeds were confirmed [ 22 ]. The pharmacological uses of linseed were applied in vivo, where the topical application authenticated its healing properties, mainly in the diabetic animal model, via minimizing the inflammation stage, stimulating re-epithelialization development, improving neovascularization, and elevating the rate of surface closure [ 46 ]. In another report, Morsi et al [ 47 ] investigated the activity of the phenolic and flavonoid contents of different linseed extracts for the management of inflammation, cancer, and oxidative stress.…”

Formulation and evaluation of a novel herbal trio gel containing flax seed extract, carbopol and carboxymethyl cellulose

“…"Ayurveda" (Ayur - Life and Veda - Science), an Indian medicine system, has been utilised successfully to treat a variety of systemic diseases [ 12 ]. Since Vedic times, people have been using natural extracts to cure a variety of health problems all across the world.…”

“…Flaxseed is a traditional awe-inspiring food that has been used in Ayurvedic therapy for thousands of years. Flaxseed ( Linum usitatissimum ), sometimes known as flax or linseed, is a plant in the Linaceae family that is high in omega-3 polyunsaturated fatty acids (PUFA), moisture, oil, calories, fibres, proteins, carbs, vitamins, and minerals [ 12 ]. It is also notable for its exceptional nutritional profile, which includes anti-bacterial, anti-inflammatory, anti-thrombotic, anti-cancer, and anti-arrhythmic properties [ 13 ] [ 14 ].…”