Histopathological and Histochemical Assessment of Monosodium Glutamate-Induced Hepatic Toxicity and the Amelioration with Propolis.

Abstract

Introduction: Monosodium glutamate (MSG) is utilized to enhance appetite and there is rising solicitude that MSG has a role in the evolution of hepatic disturbances. Propolis has been notified to have a considerable role in the amelioration of hepatic toxicity. Aim: The aim of this study is to investigate the protective effect of propolis on hepatotoxicity induced by MSG. Materials and methods: Forty-eight male rats were divided into four equal groups (Gp). GpI was served as a control, GpII was treated orally with propolis (90mg/kg bw) and GpIII was treated with MSG (97 mg/kg bw). GpIV was treated with MSG and propolis in the same doses as GpII and GpIII. All agents were administrated orally once daily for 6 weeks. Then, rats were sacrificed and blood samples were collected for biochemical analysis. Liver tissues were harvested for histopathological and histochemical examinations with determination of oxidative markers. Results: The results showed that the levels of ALT, AST, and ALP significantly augmented in MSG-treated rats whereas serum albumin level significantly declined. Rats' exposure to MSG increased oxidative parameters; malondialdehyde (MDA) in the tissue, in parallel with decreased antioxidant enzymes levels as well as histopathological damages in the hepatic tissue. The administration of propolis highly mitigated the hepatotoxic influences of MSG based on biochemical and histological alterations. Conclusion: In summary, the study obviously indicates that MSG-induced hepatic toxicity could be protected by propolis administration and such beneficial properties could be attributed to the polyphenolic compounds present in these natural products.

The protective effect of propolis against aluminum chloride-induced hepatorenal toxicity in albino rats

“…The hyperglycemia observed in the present study is in accordance with the results of El-Demerdash (2004), Shati and Alamri (2010), and Wen et al (2012). It was suggested that increase in the plasma glucose level observed in AlC1 3 -treated group may be due to the inhibition of pancreatic B-cell activity and insufficient insulin secretion (Ibrahim et al, 2019;Yousef, 2004). In contrary, the administration of propolis, AlCl 3 combined with propolis, and administration of AlCl 3 for 1 month and the administration of AlCl 3 combined with propolis for another month showed non-significant change on the level of plasma glucose.…”

“…These results are in agreements with findings of many studies (Yeh et al, 2009;Wen, Zhao, Nirala, & Bhadauria, 2012;Al-Qayim & Saadoon, 2013) who concluded that propolis mostly recovered the action of AlCl 3 on the function and structure of the liver. Moreover, the hepatoprotective effect of propolis may be attributed directly to stabilization of redox state in the cells (El-Guendouz et al, 2017;Ibrahim et al, 2019).…”

“…The biological properties of propolis have been used in folk medicine as antiamoebic, antifungal, antibacterial, antipyretic, anti-proliferative and antiinflammatory (Banskota et al, 2002;Farooqui and Farooqui, 2010) and immuno-modulatory (Ivanovska, Dimov, Pavlova, Bankova, & Popov, 1995); antioxidant, immune-stimulating, and non-toxic natures (Cole et al, 2010). Many researchers (Andrade, da Silva Filho, Cunha, Nanayakkara, & Bastos, 2008;Ibrahim, Khalifa, Saleh, & Tammam, 2019;Peña, 2008) illustrated that propolis works as a hepatoprotective substance. Previous studies also reporting that propolis have ameliorative role against the hepatic and renal damage caused by the oxidative stress damage (Wagh, 2013).…”

Alanine Aminotransferase and Aspertate Aminotransaminase Activities in Wistar Rats Fed with Musa paradisiaca (Plantain) Stem Pulp in Aluminium Chloride Induced Hepatic Oxidative Stress

“…These results are, however, in agreement with the finding of Issa et al, (2018) who concluded that M. paradisiaca pulp mostly reversed the action of CCl4 on the function and structure of the liver. Moreover, the hepatoprotective effect of M. paradisiaca may be directly linked to the stabilization of redox state in the cells (El-Guendouz et al, 2017;Ibrahim et al, 2019). The ratio of AST to ALT has more clinical utility than assessing individual elevated levels.…”