HISTOPATHOLOGIC STUDY OF CUTANEOUS FIBROSARCOMA IN DOGS (Canis familiares, Linnaeus, 1758) WITH DIFFERENT STAINING TECHNIQUES

Abstract

Fibrosarcoma is characterized as an invasive nodular tumor of mesodermal origin, with elongated normochromatic nuclei. The histopathologic analysis using specific staining techniques is efficient in detailing the neoplastic architecture and the relations with surrounding tissues. In the present study, the canine skin fibrosarcoma was histologically characterized using Hematoxilin-Eosin stain, Mallory’s Trichrome stain and Shorr’s Trichrome stain as routine staining for conjunctive tissue, Toluidine Blue to highlight the mitosis and Picrosirius-Hematoxilin Trichrome to characterize the collagen type. It was concluded that the Picrosirius stain characterized the existing collagen type as type I and III; the Toluidine Blue staining highlighted the mitoses together with Shorr’s staining, as this one also provided a marking for the conjunctive tissue and the formed bands. The Mallory’s staining technique highlighted the conjunctive tissue and the bands that are spread; the Hematoxilin-Eosin stain highlighted with emphasis the neoplastic cells.